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|Tested species reactivity||Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Rat T cell blasts.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1/50|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommend the use of acetone fixation for frozen sections. For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
CD44 cell surface antigen is a 100kDa glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
CD44 antigen; Cell surface glycoprotein CD44; Cell surface glycoprotein CD44 (hyaluronate binding protein); ECMR-III; extracellular matrix receptor III; GP90 lymphocyte homing adhesion receptor; GP90 lymphocyte homing/adhesion receptor; hermes antigen; HUTCH-I; hyaluronate binding protein; hyaluronate receptor; PGP-1; PGP-I; phagocytic glycoprotein 1; phagocytic glycoprotein I
Cd44; CD44A; METAA; RHAMM