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Immunofluorescent analysis of H-CAM in HeLa cells using a H-CAM recombinant rabbit monoclonal antibody (Product # 701406) followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI (Image B) and actin stained with Alexa Fluor 594 phalloidin (red) (image C). Image D is a composite image showing cytoplasmic localization of H-CAM.
|Tested species reactivity||Human , Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Peptide corresponding to amino acids 411–424 of human CD44.|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1 ug/ml X 10^6 cells|
|Immunocytochemistry (ICC)||0.5-2 µg/ml|
|Immunofluorescence (IF)||0.5-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with mouse based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
CD44 is a glycoprotein and functions as an extracellular matrix adhesion molecule through its binding to hyaluronate. CD44 is expressed by hematopoietic and non-hematopoietic cells. Bone marrow myeloid cells and memory T cells express CD44 at high levels, and peripheral B and T cells can upregulate the expression of CD44 in response to certain stimulatory events.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
AL133330.1, IN, MDU3, MDU2, Pgp-1, AW146109, CDW44, LHR, Ly-24, AU023126, ECMR-III, CSPG8, MIC4, HUTCH-I, Pgp1, HERMES, AW121933, HCELL, MC56
CD44 antigen, ECMR-III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, PGP-I, extracellular matrix receptor III, hermes antigen, hyaluronate receptor, lymphocyte antigen 24, phagocytic glycoprotein 1, phagocytic glycoprotein I, Hermes antigen, cell surface glycoprotein CD44, chondroitin sulfate proteoglycan 8, epican, hematopoietic cell E- and L-selectin ligand, heparan sulfate proteoglycan, homing function and Indian blood group system, Cell surface glycoprotein CD44, hyaluronate binding protein, GP90 lymphocyte homing adhesion receptor, PGP-1