|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2b|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:10 -1:100|
|Immunohistochemistry (Frozen) (IHC (F))||1:100 -1:1000|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 2 publications below|
Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) is recommended for the staining of paraffin sections. For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq, Jul 2008]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Co-existence of epithelioid and fibroblastoid subsets in a sarcomatoid renal carcinoma cell line revealed by clonal studies.
MA5-16964 was used in flow cytometry to study the presence in a sarcomatoid renal carcinoma cell line of both epithelioid and fibroblastoid cells.
|Hsieh CH,Chen HC,Chang YH,Pang ST,Kuo ML,Chuang CK,Liao SK||Anticancer research (33:4875)||2013|
Cd44: a marker of squamous differentiation in adenosquamous neoplasms.
MA5-16964 was used in immunohistochemistry (paraffin) to test if CD44, CD44v6, and CD44v7-8 are useful markers of squamous differentiation in epithelial tumors.
|Ylagan LR,Scholes J,Demopoulos R||Archives of pathology and laboratory medicine (124:212)||2000|