Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) is recommended for the staining of paraffin sections. A suggested positive control for immunohistochemical applications is skin. For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
Mouse anti Human CD44v7 antibody, clone VFF-9 specifically recognizes an epitope encoded by exon v7 on the variant portion of human CD44.
CD44 cell surface antigen is a 100kDa glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential.
Protein Aliases: AL133330.1; CD44; CD44 antigen; CD44 splice variant; CD44 var; CD44 variant; CD44v7; CDw44; cell surface glycoprotein CD44; chondroitin sulfate proteoglycan 8; ECMR-III; ECMRIII; Epican; Extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; H-CAM v7; hematopoietic cell E- and L-selectin ligand; Heparan sulfate proteoglycan; Hermes antigen; homing function and Indian blood group system; HUTCH-I; HUTCHI; Hyaluronate receptor; MGC10468; MUTCH-I; PGP I; PGP-1; PGP-I; Phagocytic glycoprotein 1; Phagocytic glycoprotein I; soluble CD44
Gene Aliases: CD44; CDW44; CSPG8; ECMR-III; HCELL; HUTCH-I; IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1
UniProt ID: (Human) P16070
Entrez Gene ID: (Human) 960
Molecular Function: transmembrane signal receptor