Invitrogen

CD45.2 Monoclonal Antibody (104), eBioscience™

Product Image Gallery

CD45.2 Antibody in Flow Cytometry (Flow) — Staining of C57Bl/6 splenocytes with 0.25 µg of Mouse IgG2a Isotype Control Purified (Product # 14-4724-82) (open histogram), or 0.25 µg of Anti-Mouse CD45-2 Purified followed by Anti-Mouse IgG FITC (Product # 11-4011-85) (closed histogram). Total viable cells were used for analysis.

CD45.2 Antibody in Immunohistochemistry (IHC) — Fig. 2 Vascular congestion in IL-10 KO mice with malaria includes thrombi-containing monocytes and T cells. Immunofluorescent staining of fixed, frozen brain sections (30 mum) from P. chabaudi -infected IL-10 KO mice (day 8 p.i., n = 4 mice). a Confocal images (x 40) of IL-10 KO brain stained for fibrin (red). b Successive single-plane confocal images (x 40) of a 30-mum z-stack showing complete occlusion of a large vessel with residual fibrinogen (red). c Immunofluorescence staining of IL-10 KO brains showing fibrin staining of blood vessels (red) and leukocytes expressing CD45 (green, x 60) and d CD11b (green, x 40). e CTV + CD4 T cells (blue) from infected IL-10 KO mice were adoptively transferred into infection-matched IL-10 KO (day 7 p.i.) recipients 3.5 h before sacrifice. Frozen brain sections (day 7 p.i.) were stained for fibrin (red). Max intensity projection of a 30-mum z-stack (x 240) displayed from brain tissue of IL-10 KO mice co-stained with WT control samples ( n = 3-4 mice per group). Scale bars represent 30 mum ( a ), 50 mum ( b - d ), or 10 mum ( d )

CD45.2 Antibody in Western Blot (WB) — Published figure using CD45.2 monoclonal antibody (Product # 14-0454-82) in Western Blot

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

Flow Cytometry (Flow)

0.5 µg/test

View 98 publications 98 publications

Immunoprecipitation (IP)

Assay-Dependent

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Mouse

Published species

Fish, Human, Mouse

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

104

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

RRID

AB_467261

Product Specific Information

Description: The 104 monoclonal antibody reacts with the mouse CD45 molecule, the leukocyte common antigen (LCA) in CD45.2-expressing mouse strains. The strains that express CD45.2 include the most commonly used mouse strains C57BL/6, BALB/c, C58, DBA/1, DBA/2, C3H/He, CBA, 129, A and AKR. CD45.2 is expressed by all leukocytes in these strains.

Applications Reported: The 104 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue sections. (Biotinylated 104 is recommended for IHC, cat. 13-0454).

Applications Tested: The 104 antibody has been tested by flow cytometric analysis of mouse splenocytes and can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD45.2 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45.2 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45.2 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45.2 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CD45; CD45 antigen; GP180; L-CA; LCA; Leukocyte common antigen; Ly-5; LY5; Lymphocyte antigen 5; lymphocyte common antigen; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein

Gene Aliases: B220; Cd45; CD45R; L-CA; loc; Ly-5; Lyt-4; Ptprc; T200

UniProt ID: (Mouse) Q78EF1

Entrez Gene ID: (Mouse) 19264

Molecular Function: protein phosphatase protein modifying enzyme