Invitrogen

CD45.2 Monoclonal Antibody (104), eBioscience™

Product Image Gallery

CD45.2 Antibody — Figure 7. Kindlin-3-deficient T cell progenitors home to the fetal thymus and further develop into SP T cells ex vivo. ( A ) Schematic description of the thymus attraction experiment. ( B ) Representative images of the thymus attraction experiment at the beginning and 40 hr after starting the experiment. Lin neg fetal liver cells from control or Fermt3 -/- embryos (both CD45.2 + ) are seen in the upper right corner of the images, a dGuo-treated wild-type fetal thymus lobe (FT, CD45.1 + ) is located at the lower left corner. Scale bar 250 um. ( C ) Schematic description of the fetal thymus organ culture. ( D ) dGuo-treated fetal thymus lobes seeded with fetal liver cells as performed in ( B ) were further cultured for 18 days. Cells were stained for CD45.1, CD45.2, CD4, and CD8. Numbers within the representative FACS plots indicate cell percentages.

CD45.2 Antibody in Flow Cytometry (Flow) — Staining of C57Bl/6 splenocytes with 0.25 µg of Mouse IgG2a Isotype Control Purified (Product # 14-4724-82) (open histogram), or 0.25 µg of Anti-Mouse CD45-2 Purified followed by Anti-Mouse IgG FITC (Product # 11-4011-85) (closed histogram). Total viable cells were used for analysis.

Product Details

Tested Applications

Dilution

Flow Cytometry (Flow)

0.5 µg/test

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

Immunoprecipitation (IP)

Assay-Dependent

Published Applications

Flow Cytometry (Flow)

See 82 publications below

Immunofluorescence (IF)

See 3 publications below

Miscellaneous PubMed (MISC)

See 2 publications below

Western Blot (WB)

See 1 publication below

Product Specifications

Species Reactivity

Mouse

Published species

Fish, Human, Mouse

Host / Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

104

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

RRID

AB_467261

Product Specific Information

Description: The 104 monoclonal antibody reacts with the mouse CD45 molecule, the leukocyte common antigen (LCA) in CD45.2-expressing mouse strains. The strains that express CD45.2 include the most commonly used mouse strains C57BL/6, BALB/c, C58, DBA/1, DBA/2, C3H/He, CBA, 129, A and AKR. CD45.2 is expressed by all leukocytes in these strains.

Applications Reported: The 104 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue sections. (Biotinylated 104 is recommended for IHC, cat. 13-0454).

Applications Tested: The 104 antibody has been tested by flow cytometric analysis of mouse splenocytes and can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD45.2 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45.2 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45.2 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45.2 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: B220; c polypeptide; CD45; CD45 antigen; CD45R; GP180; L-CA; LCA; Leukocyte common antigen; Ly-5; LY5; Lymphocyte antigen 5; lymphocyte common antigen; protein tyrosine phosphatase; PTPRC; receptor type; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen

Gene Aliases: B220; Cd45; CD45R; L-CA; loc; Ly-5; Lyt-4; Ptprc; T200

UniProt ID: (Mouse) P06800

Entrez Gene ID: (Mouse) 19264

Molecular Function: hydrolase phosphatase protein phosphatase