Invitrogen

CD45 Monoclonal Antibody (30-F11), Super Bright™ 780, eBioscience™

Product Image Gallery

CD45 Antibody in Flow Cytometry (Flow) — BALB/c mouse bone marrow cells were stained with TER-119 Monoclonal Antibody, FITC (Product # 11-5921-82) and 0.25 µg of Rat IgG2b kappa Isotype Control, Super Bright 780 (Product # 78-4031-82) (left) or 0.25 µg of CD45 Monoclonal Antibody, Super Bright 780 (right). Cells in the lymphocyte gate were used for analysis.

CD45 Antibody in Immunocytochemistry (ICC/IF) — FIGURE 7 miR-223-5p antagonist promoted diabetic corneal epithelial wound repair and nerve regeneration by modulating inflammatory process. (A) Immunofluorescence corneal staining reflected CD45 protein expression in the normal mice (NW group), diabetic mice (DW group), diabetic mice subconjunctivally injected with miR-223-5p antagomir (DW + antagomir group) or antagomir NC (DW + NC group) 24 h post-injury. (B) qRT-PCR results revealing the CD45 mRNA expression of the corneal epithelium in each group. (C-E) qRT-PCR detected the mRNA expression of inflammatory factors including TNF-alpha, IL-1beta, and IL-6 in the corneal epithelium of these four groups at 24 h post-wounding. (B-E) Data were analyzed by one-way ANOVA. If significant, multiple comparations were then performed. * p -value of <0.05.

CD45 Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Figure 1 Induction of EAE and IL-16 production by tissues of EAE mice. ( A ) C57BL/6J female mice were injected with MOG 35-55 emulsified in CFA, together with PTX. Clinical symptoms were recorded over the course of 26 days. Control mice received PBS + CFA + PTX immunisation. N = 15. Statistical significance was determined by one-way ANOVA with repeated measures and Bonferroni post-hoc tests. ** p < 0.01, *** p < 0.001. ( B - D ) CNS tissues were harvested from PBS control mice on day 16 and EAE mice on day 12, day 16 and day 26. Spinal cord ( B ), hippocampus ( C ) and cerebellum ( D ) tissues were stained for CD45 and counterstained with haematoxylin. Representative images are shown. The percentage of CD45 + cells was quantified for each group; results are expressed as mean +- SEM. N = 3 for all groups. Statistical significance was determined by one-way ANOVA with Bonferroni post-hoc tests. ( E ) Spleen cell suspensions prepared from day 16 of PBS or MOG 35-55 immunised mice were incubated either with media alone or media containing MOG 35-55 (50 mug/mL), or ConA (50 mug/mL). Supernatants were collected after 72 h of culture and used to detect IL-16 production by ELISA. Results are expressed as the mean +- SEM. PBS n = 4 and EAE n = 6. Statistical significance was determined by one-way ANOVA with Bonferroni post hoc tests. ( F ) Supernatants from homogenised spinal cord, cerebellum or cerebrum were collected and prepared to analyse IL-16 levels by ELISA. PBS n = 3, EAE n =

CD45 Antibody in Immunohistochemistry (IHC) — Fig. 1 Isolation and characterization of mesenchymal stem cells. a Schematic showing isolation of mouse bone marrow cells (BMCs) and its properties of self-renewal and differentiation of MSCs. b Representative microscopic images displaying isolated bone marrow cells that show circular and round-shaped morphology of BMCs; whereas, adhered MSCs show fibroblast or eye-shaped morphology. c Immunofluorescence images of MSCs showing the expression of MSC-positive markers (CD29, CD44, and SCA1). d Immunophenotypic characterization of MSCs using flow cytometer showing the absolute counts of MSC positive markers such as CD29, CD44, and SCA1, as well as expression of MSC-negative (CD34, CD45, and CD11b). Isotype controls for each antibody were used as controls and FITC/rhodamine-conjugated secondary antibodies were used for their detection. e Bar graph (quantification from four independent experiments (expressed as mean +- standard error)) showing the percentage of cells expressing the MSC-positive and negative markers respectively. f , g Immunophenotypic characterization of BMCs using flow cytometer showing expression various markers (CD29, CD44, CD45, and CD11b). Bar graph expressed as mean +- standard error shows quantification of respective markers from three independent experiments.

CD45 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA)) — Fig. 1 Generation and characterization of a genetic system for specific targeting of cavity macrophages. a Schematic figure showing CD45-Dre mediates Stop cassette removal from Gata6-iCreER and places CreER directly under Gata6 promoter. After tamoxifen (Tam), Cre-loxP recombination labels cells by tdTomato. b Intersectional genetics marks CD45 + GATA6 + cells as tdTomato + . c Schematic figure showing experimental design using cavity macrophage tracing tool CD45-Dre;Gata6-iCreER ( G6Mo-CreER ). d Flow cytometric analysis of the percentage of tdTomato + cells in macrophages from blood, pleural, and peritoneal cavity with or without Tam. e Immunostaining for tdTomato, CD11b, GATA6, F4/80 on dissociated cells from blood, pleural, or peritoneal cavity. Boxed regions are magnified. f Quantification of the percentage of tdTomato + cells in GATA6 + or F4/80 + macrophages. Data are the mean +- SD; n = 5 mice per group. g Quantification of the percentage of GATA6 + or F4/80 + macrophages in tdTomato + cells. Data are the mean +- SD; n = 5 mice per group. h FACS showing the percentage of tdTomato + cells expressing F4/80. i Whole-mount epifluorescence images and immunostaining for CD45 and tdTomato shows no resident tdTomato + macrophages in visceral organs. Each image is representative of 5 individual biological samples. Scale bars, yellow, 1 mm; white, 100 um. Source data are provided as a Source Data file.

Product Details

78-0451-82

Applications	Tested Dilution	Publications
Immunohistochemistry (IHC)	-	View 10 publications 10 publications
Immunohistochemistry (PFA fixed) (IHC (PFA))	-	View 1 publication 1 publication
Immunohistochemistry (Frozen) (IHC (F))	-	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	-	View 5 publications 5 publications
Flow Cytometry (Flow)	0.5 µg/test	View 28 publications 28 publications

Product Specifications
Species Reactivity	Mouse
Published species	Mouse
Host/Isotype	Rat / IgG2b, kappa
Recommended Isotype Control	Rat IgG2b kappa Isotype Control (eB149/10H5), Super Bright™ 780, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	30-F11
Conjugate	Super Bright™ 780 Alexa Fluor 488 Alexa Fluor 532 Alexa Fluor 561 Alexa Fluor 660 Alexa Fluor 700 APC APC-eFluor 780 Biotin Brilliant UV 737 Brilliant UV 805 eFluor 450 eFluor 506 FITC Functional Grade NovaFluor Blue 510 NovaFluor Blue 530 NovaFluor Blue 555 NovaFluor Blue 585 NovaFluor Blue 610-30S NovaFluor Blue 610-70S NovaFluor Blue 660-120S NovaFluor Blue 660-40S NovaFluor Red 660 NovaFluor Red 685
Excitation/Emission Profile	View spectra
Form	Liquid
Concentration	0.2 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2, with BSA
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_2734988

Product Specific Information

Description: The 30-F11 monoclonal antibody reacts with all isoforms of mouse CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding mature erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.

Applications Reported: This 30-F11 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 30-F11 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Super Bright 780 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 780 nm. We recommend using a 780/60 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

In some experiments, we have observed that compensation values for Super Bright 780-conjugated antibodies are higher in the violet 450/50 channel when using UltraComp eBeads microspheres (Product # 01-2222-42) as compared to single-color stained cells. In such circumstances, we would recommend setting compensation with cells. We have also observed this in some experiments using AbC Total Antibody Compensation beads (Product # A10497).

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 405 nm; Emission: 780 nm; Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

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Product References

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NPJ Regenerative medicine

Ahamad N,Sun Y,Nascimento Da Conceicao V,Xavier Paul Ezhilan CRD,Natarajan M,Singh BB

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Immunohistochemistry

Wed Oct 20 00:00:00 EDT 2021

Transcriptional Network Analysis Reveals the Role of miR-223-5p During Diabetic Corneal Epithelial Regeneration.

Frontiers in molecular biosciences

Zhang Y,Dou S,Qi X,Zhang Z,Qiao Y,Wang Y,Xie J,Jiang H,Zhang B,Zhou Q,Wang Q,Xie L

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Immunocytochemistry

Tue Sep 14 00:00:00 EDT 2021

The Absence of Extracellular Cold-Inducible RNA-Binding Protein (eCIRP) Promotes Pro-Angiogenic Microenvironmental Conditions and Angiogenesis in Muscle Tissue Ischemia.

International journal of molecular sciences

Kübler M,Beck S,Peffenköver LL,Götz P,Ishikawa-Ankerhold H,Preissner KT,Fischer S,Lasch M,Deindl E

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Immunocytochemistry

Tue Aug 31 00:00:00 EDT 2021

PPARγ inhibition boosts efficacy of PD-L1 Checkpoint Blockade Immunotherapy against Murine Melanoma in a sexually dimorphic manner.

International journal of biological sciences

Wu B,Sun X,Yuan B,Ge F,Gupta HB,Chiang HC,Li J,Hu Y,Curiel TJ,Li R

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Mon Aug 23 00:00:00 EDT 2021

A Synthetic Small Molecule F240B Decreases NLRP3 Inflammasome Activation by Autophagy Induction.

Frontiers in immunology

Wu CH,Gan CH,Li LH,Chang JC,Chen ST,Menon MP,Cheng SM,Yang SP,Ho CL,Chernikov OV,Lin CH,Lam Y,Hua KF

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Wed Jun 23 00:00:00 EDT 2021

Use of a Self-Delivering Anti-CCL3 FANA Oligonucleotide as an Innovative Approach to Target Inflammation after Spinal Cord Injury.

eNeuro

Pelisch N,Rosas Almanza J,Stehlik KE,Aperi BV,Kroner A

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Fri Jun 18 00:00:00 EDT 2021

CNS Immune Profiling in a Dengue Virus-Infected Immunocompetent Outbred ICR Mice Strain.

Frontiers in cellular and infection microbiology

Shen TJ,Chen CL,Jhan MK,Tseng PC,Lin CF

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Thu Jun 17 00:00:00 EDT 2021

Intercellular Interactions of an Adipogenic CXCL12-Expressing Stromal Cell Subset in Murine Bone Marrow.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Matsushita Y,Chu AKY,Ono W,Welch JD,Ono N

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Immunocytochemistry

Tue Jun 01 00:00:00 EDT 2021

C3 Deficiency Leads to Increased Angiogenesis and Elevated Pro-Angiogenic Leukocyte Recruitment in Ischemic Muscle Tissue.

International journal of molecular sciences

Götz P,Braumandl A,Kübler M,Kumaraswami K,Ishikawa-Ankerhold H,Lasch M,Deindl E

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Immunohistochemistry

Fri May 28 00:00:00 EDT 2021

Increased Levels of IL-16 in the Central Nervous System during Neuroinflammation Are Associated with Infiltrating Immune Cells and Resident Glial Cells.

Biology

Hridi SU,Barbour M,Wilson C,Franssen AJ,Harte T,Bushell TJ,Jiang HR

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Immunohistochemistry (Frozen)

Thu May 27 00:00:00 EDT 2021

VISTA regulates microglia homeostasis and myelin phagocytosis, and is associated with MS lesion pathology.

Acta neuropathologica communications

Borggrewe M,Kooistra SM,Wesseling EM,Gierschek FL,Brummer ML,Nowak EC,Medeiros-Furquim T,Otto TA,Lee SW,Noelle RJ,Eggen BJL,Laman JD

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Immunohistochemistry

Tue May 18 00:00:00 EDT 2021

Genetic fate-mapping reveals surface accumulation but not deep organ invasion of pleural and peritoneal cavity macrophages following injury.

Nature communications

Jin H,Liu K,Tang J,Huang X,Wang H,Zhang Q,Zhu H,Li Y,Pu W,Zhao H,He L,Li Y,Zhang S,Zhang Z,Zhao Y,Qin Y,Pflanz S,Kasmi KEI,Zhang W,Liu Z,Ginhoux F,Ji Y,He B,Wang L,Zhou B

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Immunohistochemistry (PFA fixed)

Mon May 17 00:00:00 EDT 2021

Absence of Cold-Inducible RNA-Binding Protein (CIRP) Promotes Angiogenesis and Regeneration of Ischemic Tissue by Inducing M2-Like Macrophage Polarization.

Biomedicines

Kübler M,Beck S,Fischer S,Götz P,Kumaraswami K,Ishikawa-Ankerhold H,Lasch M,Deindl E

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Immunohistochemistry

Wed Apr 07 00:00:00 EDT 2021

Uptake and trafficking of different sized PLGA nanoparticles by dendritic cells in imiquimod-induced psoriasis-like mice model.

Acta pharmaceutica Sinica. B

Lin Z,Xi L,Chen S,Tao J,Wang Y,Chen X,Li P,Wang Z,Zheng Y

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

Induction of alarmin S100A8/A9 mediates activation of aberrant neutrophils in the pathogenesis of COVID-19.

Cell host & microbe

Guo Q,Zhao Y,Li J,Liu J,Yang X,Guo X,Kuang M,Xia H,Zhang Z,Cao L,Luo Y,Bao L,Wang X,Wei X,Deng W,Wang N,Chen L,Chen J,Zhu H,Gao R,Qin C,Wang X,You F

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Wed Feb 10 00:00:00 EST 2021

Therapeutic Potential of Mesenchymal Stem Cells in a Pre-Clinical Model of Diabetic Kidney Disease and Obesity.

International journal of molecular sciences

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Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Thu Feb 04 00:00:00 EST 2021

A novel Cytochrome P450 26A1 expressing NK cell subset at the mouse maternal-foetal interface.

Journal of cellular and molecular medicine

Wei DP,Li DD,Gu AQ,Ji WH,Yang Y,Peng JP

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Immunocytochemistry

Mon Feb 01 00:00:00 EST 2021

Suppression of mucosal Th17 memory responses by acellular pertussis vaccines enhances nasal Bordetella pertussis carriage.

NPJ vaccines

Dubois V,Chatagnon J,Thiriard A,Bauderlique-Le Roy H,Debrie AS,Coutte L,Locht C

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Fri Jan 08 00:00:00 EST 2021

Differential neurovirulence of Usutu virus lineages in mice and neuronal cells.

Journal of neuroinflammation

Clé M,Constant O,Barthelemy J,Desmetz C,Martin MF,Lapeyre L,Cadar D,Savini G,Teodori L,Monaco F,Schmidt-Chanasit J,Saiz JC,Gonzales G,Lecollinet S,Beck C,Gosselet F,Van de Perre P,Foulongne V,Salinas S,Simonin Y

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Immunohistochemistry

Wed Jan 06 00:00:00 EST 2021

Analysis of the immune response to sciatic nerve injury identifies efferocytosis as a key mechanism of nerve debridement.

eLife

Kalinski AL,Yoon C,Huffman LD,Duncker PC,Kohen R,Passino R,Hafner H,Johnson C,Kawaguchi R,Carbajal KS,Jara JS,Hollis E,Geschwind DH,Segal BM,Giger RJ

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Wed Dec 02 00:00:00 EST 2020

Detection of Synaptic Proteins in Microglia by Flow Cytometry.

Frontiers in molecular neuroscience

Brioschi S,d'Errico P,Amann LS,Janova H,Wojcik SM,Meyer-Luehmann M,Rajendran L,Wieghofer P,Paolicelli RC,Biber K

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Tue Nov 03 00:00:00 EST 2020

Human Placental MSC-Secreted IL-1β Enhances Neutrophil Bactericidal Functions during Hypervirulent Klebsiella Infection.

Cell reports

Wang LT,Wang HH,Chiang HC,Huang LY,Chiu SK,Siu LK,Liu KJ,Yen ML,Yen BL

78-0451 was used in Flow cytometry/Cell sorting to assess whether multilineage mesenchymal stem cells (MSCs), best known for immunomodulation toward T cells, could be therapeutic for highly virulent bacterial infections via modulation of polymorphonuclear neutrophils.

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Resolvin D1 supports skeletal myofiber regeneration via actions on myeloid and muscle stem cells.

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Markworth JF,Brown LA,Lim E,Floyd C,Larouche J,Castor-Macias JA,Sugg KB,Sarver DC,Macpherson PC,Davis C,Aguilar CA,Maddipati KR,Brooks SV

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

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Ovarian granulosa cell tumor characterization identifies FOXL2 as an immunotherapeutic target.

JCI insight

Pierini S,Tanyi JL,Simpkins F,George E,Uribe-Herranz M,Drapkin R,Burger R,Morgan MA,Facciabene A

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Thu Aug 20 00:00:00 EDT 2020

Butyrophilin-like proteins display combinatorial diversity in selecting and maintaining signature intraepithelial γδ T cell compartments.

Nature communications

Jandke A,Melandri D,Monin L,Ushakov DS,Laing AG,Vantourout P,East P,Nitta T,Narita T,Takayanagi H,Feederle R,Hayday A

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Tue Jul 28 00:00:00 EDT 2020

Stromal CCL2 Signaling Promotes Mammary Tumor Fibrosis through Recruitment of Myeloid-Lineage Cells.

Cancers

Kuziel G,Thompson V,D'Amato JV,Arendt LM

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Tue Jul 28 00:00:00 EDT 2020

Quantitative single-cell interactomes in normal and virus-infected mouse lungs.

Disease models & mechanisms

Cain MP,Hernandez BJ,Chen J

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Immunocytochemistry

Fri Jun 26 00:00:00 EDT 2020

A RUNX2 stabilization pathway mediates physiologic and pathologic bone formation.

Nature communications

Kim JM,Yang YS,Park KH,Ge X,Xu R,Li N,Song M,Chun H,Bok S,Charles JF,Filhol-Cochet O,Boldyreff B,Dinter T,Yu PB,Kon N,Gu W,Takarada T,Greenblatt MB,Shim JH

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Fri May 08 00:00:00 EDT 2020

Ly6c^Lo non-classical monocytes promote resolution of rhesus rotavirus-mediated perinatal hepatic inflammation.

Scientific reports

Alkhani A,Levy CS,Tsui M,Rosenberg KA,Polovina K,Mattis AN,Mack M,Van Dyken S,Wang BM,Maher JJ,Nijagal A

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Tue Apr 28 00:00:00 EDT 2020

Inhibition of formyl peptide receptors improves the outcome in a mouse model of Alzheimer disease.

Journal of neuroinflammation

Schröder N,Schaffrath A,Welter JA,Putzka T,Griep A,Ziegler P,Brandt E,Samer S,Heneka MT,Kaddatz H,Zhan J,Kipp E,Pufe T,Tauber SC,Kipp M,Brandenburg LO

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Fri Apr 24 00:00:00 EDT 2020

Study of Usutu virus neuropathogenicity in mice and human cellular models.

PLoS neglected tropical diseases

Clé M,Barthelemy J,Desmetz C,Foulongne V,Lapeyre L,Bolloré K,Tuaillon E,Erkilic N,Kalatzis V,Lecollinet S,Beck C,Pirot N,Glasson Y,Gosselet F,Alvarez Martinez MT,Van de Perre P,Salinas S,Simonin Y

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Immunohistochemistry

Wed Apr 01 00:00:00 EDT 2020

Therapeutic modulation of phagocytosis in glioblastoma can activate both innate and adaptive antitumour immunity.

Nature communications

von Roemeling CA,Wang Y,Qie Y,Yuan H,Zhao H,Liu X,Yang Z,Yang M,Deng W,Bruno KA,Chan CK,Lee AS,Rosenfeld SS,Yun K,Johnson AJ,Mitchell DA,Jiang W,Kim BYS

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Immunohistochemistry

Fri Mar 20 00:00:00 EDT 2020

Membrane expression of the estrogen receptor ERα is required for intercellular communications in the mammary epithelium.

Development (Cambridge, England)

Gagniac L,Rusidzé M,Boudou F,Cagnet S,Adlanmerini M,Jeannot P,Gaide N,Giton F,Besson A,Weyl A,Gourdy P,Raymond-Letron I,Arnal JF,Brisken C,Lenfant F

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Wed Mar 11 00:00:00 EDT 2020

Atherogenesis in the Carotid Artery with and without Interrupted Blood Flow of Two Hyperlipidemic Mouse Strains.

Journal of vascular research

Zhao J,Huangfu C,Chang Z,Grainger AT,Liu Z,Shi W

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Mon Jan 27 00:00:00 EST 2020

NLRP3 inflammasome in fibroblasts links tissue damage with inflammation in breast cancer progression and metastasis.

Nature communications

Ershaid N,Sharon Y,Doron H,Raz Y,Shani O,Cohen N,Monteran L,Leider-Trejo L,Ben-Shmuel A,Yassin M,Gerlic M,Ben-Baruch A,Pasmanik-Chor M,Apte R,Erez N

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Thu Sep 26 00:00:00 EDT 2019

FAK activity sustains intrinsic and acquired ovarian cancer resistance to platinum chemotherapy.

eLife

Diaz Osterman CJ,Ozmadenci D,Kleinschmidt EG,Taylor KN,Barrie AM,Jiang S,Bean LM,Sulzmaier FJ,Jean C,Tancioni I,Anderson K,Uryu S,Cordasco EA,Li J,Chen XL,Fu G,Ojalill M,Rappu P,Heino J,Mark AM,Xu G,Fisch KM,Kolev VN,Weaver DT,Pachter JA,Győrffy B,McHale MT,Connolly DC,Molinolo A,Stupack DG,Schlaepfer DD

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Tue Sep 03 00:00:00 EDT 2019

Dietary calories and lipids synergistically shape adipose tissue cellularity during postnatal growth.

Molecular metabolism

Meln I,Wolff G,Gajek T,Koddebusch J,Lerch S,Harbrecht L,Hong W,Bayindir-Buchhalter I,Krunic D,Augustin HG,Vegiopoulos A

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Sat Jun 01 00:00:00 EDT 2019

Distinct Tie2 tyrosine phosphorylation sites dictate phenotypic switching in endothelial progenitor cells.

Journal of cellular physiology

Siavashi V,Nassiri SM,Rahbarghazi R,Mohseni Z,Sharifi AM

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Wed May 01 00:00:00 EDT 2019

CSF1R antagonism limits local restimulation of antiviral CD8⁺ T cells during viral encephalitis.

Journal of neuroinflammation

Funk KE,Klein RS

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Thu Jan 31 00:00:00 EST 2019

Nur77 variants solely comprising the amino-terminal domain activate hypoxia-inducible factor-1α and affect bone marrow homeostasis in mice and humans.

The Journal of biological chemistry

Koenis DS,Medzikovic L,Vos M,Beldman TJ,van Loenen PB,van Tiel CM,Hamers AAJ,Otermin Rubio I,de Waard V,de Vries CJM

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Immunohistochemistry

Fri Sep 28 00:00:00 EDT 2018

Endothelial cells in the innate response to allergens and initiation of atopic asthma.

The Journal of clinical investigation

Asosingh K,Weiss K,Queisser K,Wanner N,Yin M,Aronica M,Erzurum S

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Immunohistochemistry

Mon Jul 02 00:00:00 EDT 2018

Endothelial cells in the innate response to allergens and initiation of atopic asthma.

The Journal of clinical investigation

Asosingh K,Weiss K,Queisser K,Wanner N,Yin M,Aronica M,Erzurum S

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Immunohistochemistry

Mon Jul 02 00:00:00 EDT 2018

Dimethyl fumarate treatment after traumatic brain injury prevents depletion of antioxidative brain glutathione and confers neuroprotection.

Journal of neurochemistry

Krämer T,Grob T,Menzel L,Hirnet T,Griemert E,Radyushkin K,Thal SC,Methner A,Schaefer MKE

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Immunohistochemistry

Fri Dec 01 00:00:00 EST 2017

The Nuclear Orphan Receptor NR2F6 Is a Central Checkpoint for Cancer Immune Surveillance.

Cell reports

Hermann-Kleiter N,Klepsch V,Wallner S,Siegmund K,Klepsch S,Tuzlak S,Villunger A,Kaminski S,Pfeifhofer-Obermair C,Gruber T,Wolf D,Baier G

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Tue Sep 29 00:00:00 EDT 2015

Gli1 deletion prevents Helicobacter-induced gastric metaplasia and expansion of myeloid cell subsets.

PloS one

El-Zaatari M,Kao JY,Tessier A,Bai L,Hayes MM,Fontaine C,Eaton KA,Merchant JL

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Tue Sep 10 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: CD45; L-CA; Leukocyte common antigen; Ly-5; Lymphocyte antigen 5; lymphocyte common antigen; Receptor-type tyrosine-protein phosphatase C; T200; T220 and B220

Gene Aliases: B220; Cd45; CD45R; L-CA; loc; Ly-5; Lyt-4; Ptprc; T200

UniProt ID: (Mouse) Q78EF1

Entrez Gene ID: (Mouse) 19264

Molecular Function: protein phosphatase protein modifying enzyme