Invitrogen

CD45 Monoclonal Antibody (30-F11), Super Bright™ 780, eBioscience™

Product Image Gallery

CD45 Antibody in Flow Cytometry (Flow) — BALB/c mouse bone marrow cells were stained with TER-119 Monoclonal Antibody, FITC (Product # 11-5921-82) and 0.25 µg of Rat IgG2b kappa Isotype Control, Super Bright 780 (Product # 78-4031-82) (left) or 0.25 µg of CD45 Monoclonal Antibody, Super Bright 780 (right). Cells in the lymphocyte gate were used for analysis.

CD45 Antibody in Immunocytochemistry (ICC/IF) — Compromised self-renewal of ASCs cultured from trappc9-null mice. ASCs were isolated from WT and trappc9-deficient mice and cultured as in Methods. Both WT and KO ASCs at passage-3 were subjected to analyses. ( A ) Confocal images showed that ASCs isolated from WT and trappc9-null (KO) mice expressed mesenchymal stem cell markers and were devoid of hematopoietic CD45. Arrowheads point to cells containing two or more nuclei. Scale bars: 100 mum. ( B ) Comparison of the growth rate between WT and trappc9-deficient (KO) ASCs. ASCs were cultured at a density of 1 x 10 5 in T25 flasks and counted every 24 h for 6 days. Cell counting was performed with a hemocytometer. ( C ) Quantification of cells with two or more nuclei revealed that trappc9-null (KO) ASCs were defective in cell division. Images captured from 3 randomly chosen visual fields from cells for each genotype were used for cell counting. ( D ) Apoptosis of cells in WT and trappc9-deficient (KO) ASC cultures was examined by flow cytometry-based analysis of phosphatidylserine exposure (Annexin V-FITC staining) and plasma membrane integrity (propidium iodide staining). Shown are dot plot graphs from one of three individual analyses. Similar observations were achieved in other two experiments. Data in ( B , C ) are Mean +- SD. Each symbol represents one experiment. Statistical significance was determined by unpaired two-tailed Student's t -test: * p < 0.05; ** p < 0.01; *** p < 0.001.

CD45 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow) — 3 Fig CXCL12 is functionally important for maintaining stromal-hematopoietic coupling. ( A ) Conceptual diagram of CXCL12 conditional deletion experiment. Col2a1-cre ; Cxcl12 GFP/+ ; R26R tdTomato mice were mated with Cxcl12 fl/fl mice to generate Col2a1-cre ; Cxcl12 GFP/fl ; R26R tdTomato (Col2a1-CXCL12 cKO) and Cxcl12 GFP/fl ; R26R tdTomato (Control) mice. In Col2a1-CXCL12 cKO mice, Cxcl12-GFP + cells lose functional CXCL12 expression but still express GFP in a Cxcl12 promoter/enhancer-dependent manner. ( B ) CD45 staining of Control (left panels) and Col2a1-CXCL12 cKO (right panels) bone marrow. Dotted area = magnified view of Cxcl12 -GFP + reticular stromal cells and neighboring CD45 + hematopoietic cells. Green = Cxcl12-GFP; red = tdTomato; magenta = CD45-Alexa647; gray = DAPI. Scale bar = 10 mum. n = 4 mice per group. ( C ) Flow cytometry analysis of Control and Col2a1-CXCL12 cKO bone marrow cells. The percentage of CD45 + cells in total bone marrow cells (center) and CD45 + GFP + cells in whole GFP + fraction (right). n = 4 mice per group. Two-tailed, Mann-Whitney U test. All data are presented as mean +- SD.

CD45 Antibody in Immunohistochemistry (Frozen) (IHC (F)) — FIGURE 7 miR-223-5p antagonist promoted diabetic corneal epithelial wound repair and nerve regeneration by modulating inflammatory process. (A) Immunofluorescence corneal staining reflected CD45 protein expression in the normal mice (NW group), diabetic mice (DW group), diabetic mice subconjunctivally injected with miR-223-5p antagomir (DW + antagomir group) or antagomir NC (DW + NC group) 24 h post-injury. (B) qRT-PCR results revealing the CD45 mRNA expression of the corneal epithelium in each group. (C-E) qRT-PCR detected the mRNA expression of inflammatory factors including TNF-alpha, IL-1beta, and IL-6 in the corneal epithelium of these four groups at 24 h post-wounding. (B-E) Data were analyzed by one-way ANOVA. If significant, multiple comparations were then performed. * p -value of <0.05.

CD45 Antibody in Immunohistochemistry (IHC) — Characteristics of the corpus tissue of 6-month-old RUNX3 R122C/R122C mice. ( A ) Representative macroscopic images of the corpus of 6-month-old WT and RUNX3 R122C/R122C mice. ( B ) H&E staining of the corpus of 6-month-old WT and RUNX3 R122C/R122C mice. ( C ) IF staining for Atp4a and GSII in the corpus. ( D ) IF staining for GIF, Muc5ac, E-cadherin (E-cad), and chromogranin A (ChgA) in the corpus. ( E ) Quantification of the numbers of parietal, mucous neck, and chief cells per gland in the corpus tissues of 6-month-old WT and RUNX3 R122C/R122C mice. ( F ) qPCR for Muc6 , Atp4a , and Gif expression levels in the corpus tissues of 6-month-old WT and RUNX3 R122C/R122C mice ( n = 3 mice each). ( G ) H&E staining of the gland base in 6-month-old WT and RUNX3 R122C/R122C mice. ( H ) IF staining for CD45 and E-cad in the corpus tissues of 6-month-old WT and RUNX3 R122C/R122C mice. ( I ) qPCR for Il6beta and Tnf expression levels in the corpus tissues of 6-month-old WT and RUNX3 R122C/R122C mice ( n = 3 mice each). Scale bars : 5 mm ( A ), 100 mum ( B-D , G , and H ). Data are presented as the means +- SEM and were analyzed by the Student t test. * P < .05, *** P < .001. DAPI, 4',6-diamidino-2-phenylindole.

CD45 Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow) — Fig. 1 Isolation and characterization of mesenchymal stem cells. a Schematic showing isolation of mouse bone marrow cells (BMCs) and its properties of self-renewal and differentiation of MSCs. b Representative microscopic images displaying isolated bone marrow cells that show circular and round-shaped morphology of BMCs; whereas, adhered MSCs show fibroblast or eye-shaped morphology. c Immunofluorescence images of MSCs showing the expression of MSC-positive markers (CD29, CD44, and SCA1). d Immunophenotypic characterization of MSCs using flow cytometer showing the absolute counts of MSC positive markers such as CD29, CD44, and SCA1, as well as expression of MSC-negative (CD34, CD45, and CD11b). Isotype controls for each antibody were used as controls and FITC/rhodamine-conjugated secondary antibodies were used for their detection. e Bar graph (quantification from four independent experiments (expressed as mean +- standard error)) showing the percentage of cells expressing the MSC-positive and negative markers respectively. f , g Immunophenotypic characterization of BMCs using flow cytometer showing expression various markers (CD29, CD44, CD45, and CD11b). Bar graph expressed as mean +- standard error shows quantification of respective markers from three independent experiments.

CD45 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA)) — Fig. 1 Generation and characterization of a genetic system for specific targeting of cavity macrophages. a Schematic figure showing CD45-Dre mediates Stop cassette removal from Gata6-iCreER and places CreER directly under Gata6 promoter. After tamoxifen (Tam), Cre-loxP recombination labels cells by tdTomato. b Intersectional genetics marks CD45 + GATA6 + cells as tdTomato + . c Schematic figure showing experimental design using cavity macrophage tracing tool CD45-Dre;Gata6-iCreER ( G6Mo-CreER ). d Flow cytometric analysis of the percentage of tdTomato + cells in macrophages from blood, pleural, and peritoneal cavity with or without Tam. e Immunostaining for tdTomato, CD11b, GATA6, F4/80 on dissociated cells from blood, pleural, or peritoneal cavity. Boxed regions are magnified. f Quantification of the percentage of tdTomato + cells in GATA6 + or F4/80 + macrophages. Data are the mean +- SD; n = 5 mice per group. g Quantification of the percentage of GATA6 + or F4/80 + macrophages in tdTomato + cells. Data are the mean +- SD; n = 5 mice per group. h FACS showing the percentage of tdTomato + cells expressing F4/80. i Whole-mount epifluorescence images and immunostaining for CD45 and tdTomato shows no resident tdTomato + macrophages in visceral organs. Each image is representative of 5 individual biological samples. Scale bars, yellow, 1 mm; white, 100 um. Source data are provided as a Source Data file.

CD45 Antibody in Western Blot (WB) — Figure 5 Pro-inflammatory phenotype of microglia in the PFC of DAT-/- rats. ( A ) Levels of CNS microglial marker CD45 were unchanged among the three genotypes. Morphometric analysis of Iba1 + cells showed ( B ) an increase in the total length extension of microglial processes of DAT-/- rats, ( C ) while mean maximum radius did not vary among genotypes. ( D ) Number of branches was significantly higher in DAT-/- rats. ( E ) Sholl analysis displayed higher number of intersections in Iba1 + cells in DAT-/- PFC. Individual data points are shown for each group. Data are presented as mean +- SEM, * p < 0.05, ** p < 0.001, *** p < 0.001.

Product Details

78-0451-82

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 14 publications 14 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

View 5 publications 5 publications

Flow Cytometry (Flow)

0.5 µg/test

View 95 publications 95 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), Super Bright™ 780, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

30-F11

Conjugate

Super Bright™ 780

Excitation/Emission Max

413/780 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

RRID

AB_2734988

Product Specific Information

Description: The 30-F11 monoclonal antibody reacts with all isoforms of mouse CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding mature erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.

Applications Reported: This 30-F11 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 30-F11 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Super Bright 780 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 780 nm. We recommend using a 780/60 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

In some experiments, we have observed that compensation values for Super Bright 780-conjugated antibodies are higher in the violet 450/50 channel when using UltraComp eBeads microspheres (Product # 01-2222-42) as compared to single-color stained cells. In such circumstances, we would recommend setting compensation with cells. We have also observed this in some experiments using AbC Total Antibody Compensation beads (Product # A10497).

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 405 nm; Emission: 780 nm; Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

Panel Builder

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Comparative analysis of thoracic and abdominal aortic aneurysms across the segment and species at the single-cell level.

Frontiers in pharmacology

Wu H,Xie C,Wang R,Cheng J,Xu Q,Zhao H

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Thu Feb 02 00:00:00 EST 2023

Microglial angiotensin type 2 receptors mediate sex-specific expression of inflammatory cytokines independently of circulating estrogen.

Glia

Garrido-Gil P,Pedrosa MA,Garcia-Garrote M,Pequeño-Valtierra A,Rodríguez-Castro J,García-Souto D,Rodríguez-Pérez AI,Labandeira-Garcia JL

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2022

GPR92 activation in islet macrophages controls β cell function in a diet-induced obesity model.

The Journal of clinical investigation

de Souza CO,Paschoal VA,Sun X,Vishvanath L,Zhang Q,Shao M,Onodera T,Chen S,Joffin N,Bueno LM,Gupta RK,Oh DY

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Tue Nov 01 00:00:00 EDT 2022

Mineralocorticoid receptor antagonists and glucocorticoids differentially affect skeletal muscle inflammation and pathology in muscular dystrophy.

JCI insight

Howard ZM,Gomatam CK,Rabolli CP,Lowe J,Piepho AB,Bansal SS,Accornero F,Rafael-Fortney JA

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Mon Oct 10 00:00:00 EDT 2022

Hematopoietic-Mesenchymal Signals Regulate the Properties of Mesenchymal Stem Cells.

International journal of molecular sciences

Kanazawa S,Okada H,Riu D,Mabuchi Y,Akazawa C,Iwata J,Hoshi K,Hikita A

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Tue Jul 26 00:00:00 EDT 2022

The ZIP8/SIRT1 axis regulates alveolar progenitor cell renewal in aging and idiopathic pulmonary fibrosis.

The Journal of clinical investigation

Liang J,Huang G,Liu X,Taghavifar F,Liu N,Wang Y,Deng N,Yao C,Xie T,Kulur V,Dai K,Burman A,Rowan SC,Weigt SS,Belperio J,Stripp B,Parks WC,Jiang D,Noble PW

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Wed Jun 01 00:00:00 EDT 2022

Trappc9 Deficiency Impairs the Plasticity of Stem Cells.

International journal of molecular sciences

Usman M,Li Y,Ke Y,Chhetri G,Islam MA,Wang Z,Li X

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Immunocytochemistry

Thu Apr 28 00:00:00 EDT 2022

A Point Mutation R122C in RUNX3 Promotes the Expansion of Isthmus Stem Cells and Inhibits Their Differentiation in the Stomach.

Cellular and molecular gastroenterology and hepatology

Douchi D,Yamamura A,Matsuo J,Lee JW,Nuttonmanit N,Melissa Lim YH,Suda K,Shimura M,Chen S,Pang S,Kohu K,Kaneko M,Kiyonari H,Kaneda A,Yoshida H,Taniuchi I,Osato M,Yang H,Unno M,Bok-Yan So J,Yeoh KG,Huey Chuang LS,Bae SC,Ito Y

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Immunohistochemistry

Mon Apr 18 00:00:00 EDT 2022

Phosphatase SHP1 impedes mesenchymal stromal cell immunosuppressive capacity modulated by JAK1/STAT3 and P38 signals.

Cell & bioscience

Jiang M,Ye J,Wang X,Li N,Wang Y,Shi Y

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Thu Apr 14 00:00:00 EDT 2022

Intranasal delivery of a small-molecule ErbB inhibitor promotes recovery from acute and late-stage CNS inflammation.

JCI insight

Linnerbauer M,Lößlein L,Vandrey O,Tsaktanis T,Beer A,Naumann UJ,Panier F,Beyer T,Nirschl L,Kuramatsu JB,Winkler J,Quintana FJ,Rothhammer V

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Fri Apr 08 00:00:00 EDT 2022

Gene-selective transcription promotes the inhibition of tissue reparative macrophages by TNF.

Life science alliance

Dichtl S,Sanin DE,Koss CK,Willenborg S,Petzold A,Tanzer MC,Dahl A,Kabat AM,Lindenthal L,Zeitler L,Satzinger S,Strasser A,Mann M,Roers A,Eming SA,El Kasmi KC,Pearce EJ,Murray PJ

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Fri Apr 01 00:00:00 EDT 2022

Interleukin-10 genetically modified clinical-grade mesenchymal stromal cells markedly reinforced functional recovery after spinal cord injury via directing alternative activation of macrophages.

Cellular & molecular biology letters

Gao T,Huang F,Wang W,Xie Y,Wang B

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Thu Mar 17 00:00:00 EDT 2022

Cytochrome P450 26A1 Modulates the Polarization of Uterine Macrophages During the Peri-Implantation Period.

Frontiers in immunology

Ji WH,Li DD,Wei DP,Gu AQ,Yang Y,Peng JP

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Thu Feb 10 00:00:00 EST 2022

Senolytic elimination of Cox2-expressing senescent cells inhibits the growth of premalignant pancreatic lesions.

Gut

Kolodkin-Gal D,Roitman L,Ovadya Y,Azazmeh N,Assouline B,Schlesinger Y,Kalifa R,Horwitz S,Khalatnik Y,Hochner-Ger A,Imam A,Demma JA,Winter E,Benyamini H,Elgavish S,Khatib AA,Meir K,Atlan K,Pikarsky E,Parnas O,Dor Y,Zamir G,Ben-Porath I,Krizhanovsky V

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Immunohistochemistry

Tue Feb 01 00:00:00 EST 2022

BEX1 is a critical determinant of viral myocarditis.

PLoS pathogens

Martens CR,Dorn LE,Kenney AD,Bansal SS,Yount JS,Accornero F

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Tue Feb 01 00:00:00 EST 2022

A molybdenum oxide-based degradable nanosheet for combined chemo-photothermal therapy to improve tumor immunosuppression and suppress distant tumors and lung metastases.

Journal of nanobiotechnology

Qiu N,Yang X,Zhang Y,Zhang J,Ji J,Zhang Y,Kong X,Xi Y,Liu D,Ye L,Zhai G

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Sun Dec 19 00:00:00 EST 2021

Cardiac-derived TGF-β1 confers resistance to diet-induced obesity through the regulation of adipocyte size and function.

Molecular metabolism

Longenecker JZ,Petrosino JM,Martens CR,Hinger SA,Royer CJ,Dorn LE,Branch DA,Serrano J,Stanford KI,Kyriazis GA,Baskin KK,Accornero F

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Wed Dec 01 00:00:00 EST 2021

The IRE1/XBP1 signaling axis promotes skeletal muscle regeneration through a cell non-autonomous mechanism.

eLife

Roy A,Tomaz da Silva M,Bhat R,Bohnert KR,Iwawaki T,Kumar A

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Tue Nov 23 00:00:00 EST 2021

Deletion of mTOR in liver epithelial cells enhances hepatic metastasis of colon cancer.

The Journal of pathology

Jiao L,Eickhoff R,Egners A,Jumpertz S,Roth J,Erdem M,Kroh A,Duimel H,López-Iglesias C,Caro P,Heij LR,Schmeding M,Meierhofer D,Neumann UP,Cramer T

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Immunohistochemistry

Mon Nov 01 00:00:00 EDT 2021

Differential activation of Ca²⁺ influx channels modulate stem cell potency, their proliferation/viability and tissue regeneration.

NPJ Regenerative medicine

Ahamad N,Sun Y,Nascimento Da Conceicao V,Xavier Paul Ezhilan CRD,Natarajan M,Singh BB

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Immunohistochemistry

Wed Oct 20 00:00:00 EDT 2021

Differential activation of Ca²⁺ influx channels modulate stem cell potency, their proliferation/viability and tissue regeneration.

NPJ Regenerative medicine

Ahamad N,Sun Y,Nascimento Da Conceicao V,Xavier Paul Ezhilan CRD,Natarajan M,Singh BB

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Immunohistochemistry

Wed Oct 20 00:00:00 EDT 2021

T cell-depleting nanoparticles ameliorate bone loss by reducing activated T cells and regulating the Treg/Th17 balance.

Bioactive materials

Yang X,Zhou F,Yuan P,Dou G,Liu X,Liu S,Wang X,Jin R,Dong Y,Zhou J,Lv Y,Deng Z,Liu S,Chen X,Han Y,Jin Y

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Fri Oct 01 00:00:00 EDT 2021

Transcriptional Network Analysis Reveals the Role of miR-223-5p During Diabetic Corneal Epithelial Regeneration.

Frontiers in molecular biosciences

Zhang Y,Dou S,Qi X,Zhang Z,Qiao Y,Wang Y,Xie J,Jiang H,Zhang B,Zhou Q,Wang Q,Xie L

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Immunocytochemistry

Tue Sep 14 00:00:00 EDT 2021

The Absence of Extracellular Cold-Inducible RNA-Binding Protein (eCIRP) Promotes Pro-Angiogenic Microenvironmental Conditions and Angiogenesis in Muscle Tissue Ischemia.

International journal of molecular sciences

Kübler M,Beck S,Peffenköver LL,Götz P,Ishikawa-Ankerhold H,Preissner KT,Fischer S,Lasch M,Deindl E

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Immunocytochemistry

Tue Aug 31 00:00:00 EDT 2021

PTENα functions as an immune suppressor and promotes immune resistance in PTEN-mutant cancer.

Nature communications

Sun Y,Lu D,Yin Y,Song J,Liu Y,Hao W,Qi F,Zhang G,Zhang X,Liu L,Lin Z,Liang H,Zhao X,Jin Y,Yin Y

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Thu Aug 26 00:00:00 EDT 2021

PPARγ inhibition boosts efficacy of PD-L1 Checkpoint Blockade Immunotherapy against Murine Melanoma in a sexually dimorphic manner.

International journal of biological sciences

Wu B,Sun X,Yuan B,Ge F,Gupta HB,Chiang HC,Li J,Hu Y,Curiel TJ,Li R

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Mon Aug 23 00:00:00 EDT 2021

Decreased antiviral immune response within the central nervous system of aged mice is associated with increased lethality of West Nile virus encephalitis.

Aging cell

Funk KE,Arutyunov AD,Desai P,White JP,Soung AL,Rosen SF,Diamond MS,Klein RS

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Sun Aug 01 00:00:00 EDT 2021

Distinct skeletal stem cell types orchestrate long bone skeletogenesis.

eLife

Ambrosi TH,Sinha R,Steininger HM,Hoover MY,Murphy MP,Koepke LS,Wang Y,Lu WJ,Morri M,Neff NF,Weissman IL,Longaker MT,Chan CK

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Mon Jul 19 00:00:00 EDT 2021

RANKL from bone marrow adipose lineage cells promotes osteoclast formation and bone loss.

EMBO reports

Hu Y,Li X,Zhi X,Cong W,Huang B,Chen H,Wang Y,Li Y,Wang L,Fang C,Guo J,Liu Y,Cui J,Cao L,Weng W,Zhou Q,Wang S,Chen X,Su J

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Mon Jul 05 00:00:00 EDT 2021

NOX4 regulates macrophage apoptosis resistance to induce fibrotic progression.

The Journal of biological chemistry

Larson-Casey JL,Gu L,Kang J,Dhyani A,Carter AB

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Thu Jul 01 00:00:00 EDT 2021

Macrophage polarization by MSC-derived CXCL12 determines tumor growth.

Cellular & molecular biology letters

Babazadeh S,Nassiri SM,Siavashi V,Sahlabadi M,Hajinasrollah M,Zamani-Ahmadmahmudi M

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Sat Jun 26 00:00:00 EDT 2021

A Synthetic Small Molecule F240B Decreases NLRP3 Inflammasome Activation by Autophagy Induction.

Frontiers in immunology

Wu CH,Gan CH,Li LH,Chang JC,Chen ST,Menon MP,Cheng SM,Yang SP,Ho CL,Chernikov OV,Lin CH,Lam Y,Hua KF

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Wed Jun 23 00:00:00 EDT 2021

Visceral adipose tissue imparts peripheral macrophage influx into the hypothalamus.

Journal of neuroinflammation

Chen KE,Lainez NM,Nair MG,Coss D

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Mon Jun 21 00:00:00 EDT 2021

Use of a Self-Delivering Anti-CCL3 FANA Oligonucleotide as an Innovative Approach to Target Inflammation after Spinal Cord Injury.

eNeuro

Pelisch N,Rosas Almanza J,Stehlik KE,Aperi BV,Kroner A

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Fri Jun 18 00:00:00 EDT 2021

CNS Immune Profiling in a Dengue Virus-Infected Immunocompetent Outbred ICR Mice Strain.

Frontiers in cellular and infection microbiology

Shen TJ,Chen CL,Jhan MK,Tseng PC,Lin CF

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Thu Jun 17 00:00:00 EDT 2021

Determining the effect of aging, recovery time, and post-stroke memantine treatment on delayed thalamic gliosis after cortical infarct.

Scientific reports

Kim GS,Stephenson JM,Al Mamun A,Wu T,Goss MG,Min JW,Li J,Liu F,Marrelli SP

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Tue Jun 15 00:00:00 EDT 2021

Cellular and physiological circadian mechanisms drive diurnal cell proliferation and expansion of white adipose tissue.

Nature communications

Ribas-Latre A,Santos RB,Fekry B,Tamim YM,Shivshankar S,Mohamed AMT,Baumgartner C,Kwok C,Gebhardt C,Rivera A,Gao Z,Sun K,Heiker JT,Snyder BE,Kolonin MG,Eckel-Mahan KL

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Immunocytochemistry

Wed Jun 09 00:00:00 EDT 2021

Macrophage LAMTOR1 Deficiency Prevents Dietary Obesity and Insulin Resistance Through Inflammation-Induced Energy Expenditure.

Frontiers in cell and developmental biology

Ying L,Zhang M,Ma X,Si Y,Li X,Su J,Yin J,Bao Y

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Tue Jun 08 00:00:00 EDT 2021

Synergistic immunotherapy of glioblastoma by dual targeting of IL-6 and CD40.

Nature communications

Yang F,He Z,Duan H,Zhang D,Li J,Yang H,Dorsey JF,Zou W,Nabavizadeh SA,Bagley SJ,Abdullah K,Brem S,Zhang L,Xu X,Byrne KT,Vonderheide RH,Gong Y,Fan Y

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Tue Jun 08 00:00:00 EDT 2021

Intercellular Interactions of an Adipogenic CXCL12-Expressing Stromal Cell Subset in Murine Bone Marrow.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Matsushita Y,Chu AKY,Ono W,Welch JD,Ono N

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Immunocytochemistry

Tue Jun 01 00:00:00 EDT 2021

Intercellular Interactions of an Adipogenic CXCL12-Expressing Stromal Cell Subset in Murine Bone Marrow.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Matsushita Y,Chu AKY,Ono W,Welch JD,Ono N

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Immunocytochemistry

Tue Jun 01 00:00:00 EDT 2021

C3 Deficiency Leads to Increased Angiogenesis and Elevated Pro-Angiogenic Leukocyte Recruitment in Ischemic Muscle Tissue.

International journal of molecular sciences

Götz P,Braumandl A,Kübler M,Kumaraswami K,Ishikawa-Ankerhold H,Lasch M,Deindl E

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Immunohistochemistry

Fri May 28 00:00:00 EDT 2021

Increased Levels of IL-16 in the Central Nervous System during Neuroinflammation Are Associated with Infiltrating Immune Cells and Resident Glial Cells.

Biology

Hridi SU,Barbour M,Wilson C,Franssen AJ,Harte T,Bushell TJ,Jiang HR

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Immunohistochemistry (Frozen)

Thu May 27 00:00:00 EDT 2021

VISTA regulates microglia homeostasis and myelin phagocytosis, and is associated with MS lesion pathology.

Acta neuropathologica communications

Borggrewe M,Kooistra SM,Wesseling EM,Gierschek FL,Brummer ML,Nowak EC,Medeiros-Furquim T,Otto TA,Lee SW,Noelle RJ,Eggen BJL,Laman JD

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Immunohistochemistry

Tue May 18 00:00:00 EDT 2021

Genetic fate-mapping reveals surface accumulation but not deep organ invasion of pleural and peritoneal cavity macrophages following injury.

Nature communications

Jin H,Liu K,Tang J,Huang X,Wang H,Zhang Q,Zhu H,Li Y,Pu W,Zhao H,He L,Li Y,Zhang S,Zhang Z,Zhao Y,Qin Y,Pflanz S,Kasmi KEI,Zhang W,Liu Z,Ginhoux F,Ji Y,He B,Wang L,Zhou B

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Immunohistochemistry (PFA fixed)

Mon May 17 00:00:00 EDT 2021

Integrated OMICs unveil the bone-marrow microenvironment in human leukemia.

Cell reports

Passaro D,Garcia-Albornoz M,Diana G,Chakravarty P,Ariza-McNaughton L,Batsivari A,Borràs-Eroles C,Abarrategi A,Waclawiczek A,Ombrato L,Malanchi I,Gribben J,Bonnet D

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Tue May 11 00:00:00 EDT 2021

Mesenchymal Stromal Cell-Derived Exosomes Contribute to Epithelial Regeneration in Experimental Inflammatory Bowel Disease.

Cellular and molecular gastroenterology and hepatology

Barnhoorn MC,Plug L,Jonge ESMM,Molenkamp D,Bos E,Schoonderwoerd MJA,Corver WE,van der Meulen-de Jong AE,Verspaget HW,Hawinkels LJAC

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Mon May 10 00:00:00 EDT 2021

Apoptotic vesicles restore liver macrophage homeostasis to counteract type 2 diabetes.

Journal of extracellular vesicles

Zheng C,Sui B,Zhang X,Hu J,Chen J,Liu J,Wu D,Ye Q,Xiang L,Qiu X,Liu S,Deng Z,Zhou J,Liu S,Shi S,Jin Y

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Sat May 01 00:00:00 EDT 2021

Ablation of lysozyme M-positive cells prevents aircraft noise-induced vascular damage without improving cerebral side effects.

Basic research in cardiology

Frenis K,Helmstädter J,Ruan Y,Schramm E,Kalinovic S,Kröller-Schön S,Bayo Jimenez MT,Hahad O,Oelze M,Jiang S,Wenzel P,Sommer CJ,Frauenknecht KBM,Waisman A,Gericke A,Daiber A,Münzel T,Steven S

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Fri Apr 30 00:00:00 EDT 2021

Placenta-Expanded Stromal Cell Therapy in a Rodent Model of Simulated Weightlessness.

Cells

Rubinstein L,Paul AM,Houseman C,Abegaz M,Tabares Ruiz S,O'Neil N,Kunis G,Ofir R,Cohen J,Ronca AE,Globus RK,Tahimic CGT

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Mon Apr 19 00:00:00 EDT 2021

Absence of Cold-Inducible RNA-Binding Protein (CIRP) Promotes Angiogenesis and Regeneration of Ischemic Tissue by Inducing M2-Like Macrophage Polarization.

Biomedicines

Kübler M,Beck S,Fischer S,Götz P,Kumaraswami K,Ishikawa-Ankerhold H,Lasch M,Deindl E

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Immunohistochemistry

Wed Apr 07 00:00:00 EDT 2021

Uptake and trafficking of different sized PLGA nanoparticles by dendritic cells in imiquimod-induced psoriasis-like mice model.

Acta pharmaceutica Sinica. B

Lin Z,Xi L,Chen S,Tao J,Wang Y,Chen X,Li P,Wang Z,Zheng Y

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

The effects of postmortem delay on mouse and human microglia gene expression.

Glia

Heng Y,Dubbelaar ML,Marie SKN,Boddeke EWGM,Eggen BJL

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

Alterations of macrophage and neutrophil content in skeletal muscle of aged versus young mice.

Muscle & nerve

Kawanishi N,Machida S

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

A decrease in NAD⁺ contributes to the loss of osteoprogenitors and bone mass with aging.

NPJ aging and mechanisms of disease

Kim HN,Ponte F,Warren A,Ring R,Iyer S,Han L,Almeida M

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

Mapping the origin and fate of myeloid cells in distinct compartments of the eye by single-cell profiling.

The EMBO journal

Wieghofer P,Hagemeyer N,Sankowski R,Schlecht A,Staszewski O,Amann L,Gruber M,Koch J,Hausmann A,Zhang P,Boneva S,Masuda T,Hilgendorf I,Goldmann T,Böttcher C,Priller J,Rossi FM,Lange C,Prinz M

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Mon Mar 15 00:00:00 EDT 2021

Photoreceptor protection by mesenchymal stem cell transplantation identifies exosomal MiR-21 as a therapeutic for retinal degeneration.

Cell death and differentiation

Deng CL,Hu CB,Ling ST,Zhao N,Bao LH,Zhou F,Xiong YC,Chen T,Sui BD,Yu XR,Hu CH

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Mon Mar 01 00:00:00 EST 2021

Induction of alarmin S100A8/A9 mediates activation of aberrant neutrophils in the pathogenesis of COVID-19.

Cell host & microbe

Guo Q,Zhao Y,Li J,Liu J,Yang X,Guo X,Kuang M,Xia H,Zhang Z,Cao L,Luo Y,Bao L,Wang X,Wei X,Deng W,Wang N,Chen L,Chen J,Zhu H,Gao R,Qin C,Wang X,You F

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Wed Feb 10 00:00:00 EST 2021

Early Adolescence Prefrontal Cortex Alterations in Female Rats Lacking Dopamine Transporter.

Biomedicines

Illiano P,Leo D,Gainetdinov RR,Pardo M

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Western Blot

Fri Feb 05 00:00:00 EST 2021

Therapeutic Potential of Mesenchymal Stem Cells in a Pre-Clinical Model of Diabetic Kidney Disease and Obesity.

International journal of molecular sciences

Sávio-Silva C,Soinski-Sousa PE,Simplício-Filho A,Bastos RMC,Beyerstedt S,Rangel ÉB

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Thu Feb 04 00:00:00 EST 2021

A novel Cytochrome P450 26A1 expressing NK cell subset at the mouse maternal-foetal interface.

Journal of cellular and molecular medicine

Wei DP,Li DD,Gu AQ,Ji WH,Yang Y,Peng JP

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Immunocytochemistry

Mon Feb 01 00:00:00 EST 2021

The effect of fingolimod on regulatory T cells in a mouse model of brain ischaemia.

Journal of neuroinflammation

Malone K,Diaz Diaz AC,Shearer JA,Moore AC,Waeber C

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Sat Jan 30 00:00:00 EST 2021

Suppression of mucosal Th17 memory responses by acellular pertussis vaccines enhances nasal Bordetella pertussis carriage.

NPJ vaccines

Dubois V,Chatagnon J,Thiriard A,Bauderlique-Le Roy H,Debrie AS,Coutte L,Locht C

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Fri Jan 08 00:00:00 EST 2021

Differential neurovirulence of Usutu virus lineages in mice and neuronal cells.

Journal of neuroinflammation

Clé M,Constant O,Barthelemy J,Desmetz C,Martin MF,Lapeyre L,Cadar D,Savini G,Teodori L,Monaco F,Schmidt-Chanasit J,Saiz JC,Gonzales G,Lecollinet S,Beck C,Gosselet F,Van de Perre P,Foulongne V,Salinas S,Simonin Y

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Immunohistochemistry

Wed Jan 06 00:00:00 EST 2021

Anti-adipogenic signals at the onset of obesity-related inflammation in white adipose tissue.

Cellular and molecular life sciences : CMLS

Caputo T,Tran VDT,Bararpour N,Winkler C,Aguileta G,Trang KB,Giordano Attianese GMP,Wilson A,Thomas A,Pagni M,Guex N,Desvergne B,Gilardi F

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

Loss of p53 in mesenchymal stem cells promotes alteration of bone remodeling through negative regulation of osteoprotegerin.

Cell death and differentiation

Velletri T,Huang Y,Wang Y,Li Q,Hu M,Xie N,Yang Q,Chen X,Chen Q,Shou P,Gan Y,Candi E,Annicchiarico-Petruzzelli M,Agostini M,Yang H,Melino G,Shi Y,Wang Y

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

Dysfunction of B Cell Leading to Failure of Immunoglobulin Response Is Ameliorated by Dietary Silk Peptide in 14-Month-Old C57BL/6 Mice.

Frontiers in nutrition

Chei S,Oh HJ,Lee K,Jin H,Lee JY,Lee BY

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Fri Dec 18 00:00:00 EST 2020

Eosinophils improve cardiac function after myocardial infarction.

Nature communications

Liu J,Yang C,Liu T,Deng Z,Fang W,Zhang X,Li J,Huang Q,Liu C,Wang Y,Yang D,Sukhova GK,Lindholt JS,Diederichsen A,Rasmussen LM,Li D,Newton G,Luscinskas FW,Liu L,Libby P,Wang J,Guo J,Shi GP

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Wed Dec 16 00:00:00 EST 2020

Analysis of the immune response to sciatic nerve injury identifies efferocytosis as a key mechanism of nerve debridement.

eLife

Kalinski AL,Yoon C,Huffman LD,Duncker PC,Kohen R,Passino R,Hafner H,Johnson C,Kawaguchi R,Carbajal KS,Jara JS,Hollis E,Geschwind DH,Segal BM,Giger RJ

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Wed Dec 02 00:00:00 EST 2020

Apoptotic bodies derived from mesenchymal stem cells promote cutaneous wound healing via regulating the functions of macrophages.

Stem cell research & therapy

Liu J,Qiu X,Lv Y,Zheng C,Dong Y,Dou G,Zhu B,Liu A,Wang W,Zhou J,Liu S,Liu S,Gao B,Jin Y

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Fri Nov 27 00:00:00 EST 2020

Enhancer remodeling promotes tumor-initiating activity in NRF2-activated non-small cell lung cancers.

Nature communications

Okazaki K,Anzawa H,Liu Z,Ota N,Kitamura H,Onodera Y,Alam MM,Matsumaru D,Suzuki T,Katsuoka F,Tadaka S,Motoike I,Watanabe M,Hayasaka K,Sakurada A,Okada Y,Yamamoto M,Suzuki T,Kinoshita K,Sekine H,Motohashi H

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Fri Nov 20 00:00:00 EST 2020

Detection of Synaptic Proteins in Microglia by Flow Cytometry.

Frontiers in molecular neuroscience

Brioschi S,d'Errico P,Amann LS,Janova H,Wojcik SM,Meyer-Luehmann M,Rajendran L,Wieghofer P,Paolicelli RC,Biber K

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Tue Nov 03 00:00:00 EST 2020

Chronic alcohol-induced neuroinflammation involves CCR2/5-dependent peripheral macrophage infiltration and microglia alterations.

Journal of neuroinflammation

Lowe PP,Morel C,Ambade A,Iracheta-Vellve A,Kwiatkowski E,Satishchandran A,Furi I,Cho Y,Gyongyosi B,Catalano D,Lefebvre E,Fischer L,Seyedkazemi S,Schafer DP,Szabo G

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Fri Oct 09 00:00:00 EDT 2020

Small GTPase RAB6 deficiency promotes alveolar progenitor cell renewal and attenuates PM2.5-induced lung injury and fibrosis.

Cell death & disease

Yang L,Liu G,Li X,Xia Z,Wang Y,Lin W,Zhang W,Zhang W,Li X

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Sun Oct 04 00:00:00 EDT 2020

Sema3A and HIF1α co-overexpressed iPSC-MSCs/HA scaffold facilitates the repair of calvarial defect in a mouse model.

Journal of cellular physiology

Li J,Wang T,Li C,Wang Z,Wang P,Zheng L

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

Human Placental MSC-Secreted IL-1β Enhances Neutrophil Bactericidal Functions during Hypervirulent Klebsiella Infection.

Cell reports

Wang LT,Wang HH,Chiang HC,Huang LY,Chiu SK,Siu LK,Liu KJ,Yen ML,Yen BL

78-0451 was used in Flow cytometry/Cell sorting to assess whether multilineage mesenchymal stem cells (MSCs), best known for immunomodulation toward T cells, could be therapeutic for highly virulent bacterial infections via modulation of polymorphonuclear neutrophils.

Tue Sep 29 00:00:00 EDT 2020

Resolvin D1 supports skeletal myofiber regeneration via actions on myeloid and muscle stem cells.

JCI insight

Markworth JF,Brown LA,Lim E,Floyd C,Larouche J,Castor-Macias JA,Sugg KB,Sarver DC,Macpherson PC,Davis C,Aguilar CA,Maddipati KR,Brooks SV

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Thu Sep 17 00:00:00 EDT 2020

Ovarian granulosa cell tumor characterization identifies FOXL2 as an immunotherapeutic target.

JCI insight

Pierini S,Tanyi JL,Simpkins F,George E,Uribe-Herranz M,Drapkin R,Burger R,Morgan MA,Facciabene A

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Thu Aug 20 00:00:00 EDT 2020

The Cytokine TGF-β Induces Interleukin-31 Expression from Dermal Dendritic Cells to Activate Sensory Neurons and Stimulate Wound Itching.

Immunity

Xu J,Zanvit P,Hu L,Tseng PY,Liu N,Wang F,Liu O,Zhang D,Jin W,Guo N,Han Y,Yin J,Cain A,Hoon MA,Wang S,Chen W

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Tue Aug 18 00:00:00 EDT 2020

Stromal CCL2 Signaling Promotes Mammary Tumor Fibrosis through Recruitment of Myeloid-Lineage Cells.

Cancers

Kuziel G,Thompson V,D'Amato JV,Arendt LM

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Tue Jul 28 00:00:00 EDT 2020

Butyrophilin-like proteins display combinatorial diversity in selecting and maintaining signature intraepithelial γδ T cell compartments.

Nature communications

Jandke A,Melandri D,Monin L,Ushakov DS,Laing AG,Vantourout P,East P,Nitta T,Narita T,Takayanagi H,Feederle R,Hayday A

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Tue Jul 28 00:00:00 EDT 2020

Quantitative single-cell interactomes in normal and virus-infected mouse lungs.

Disease models & mechanisms

Cain MP,Hernandez BJ,Chen J

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Immunocytochemistry

Fri Jun 26 00:00:00 EDT 2020

A RUNX2 stabilization pathway mediates physiologic and pathologic bone formation.

Nature communications

Kim JM,Yang YS,Park KH,Ge X,Xu R,Li N,Song M,Chun H,Bok S,Charles JF,Filhol-Cochet O,Boldyreff B,Dinter T,Yu PB,Kon N,Gu W,Takarada T,Greenblatt MB,Shim JH

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Fri May 08 00:00:00 EDT 2020

Resveratrol rescues TNF‑α‑induced inhibition of osteogenesis in human periodontal ligament stem cells via the ERK1/2 pathway.

Molecular medicine reports

Yuan J,Wang X,Ma D,Gao H,Zheng D,Zhang J

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Fri May 01 00:00:00 EDT 2020

Ly6c^Lo non-classical monocytes promote resolution of rhesus rotavirus-mediated perinatal hepatic inflammation.

Scientific reports

Alkhani A,Levy CS,Tsui M,Rosenberg KA,Polovina K,Mattis AN,Mack M,Van Dyken S,Wang BM,Maher JJ,Nijagal A

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Tue Apr 28 00:00:00 EDT 2020

Inhibition of formyl peptide receptors improves the outcome in a mouse model of Alzheimer disease.

Journal of neuroinflammation

Schröder N,Schaffrath A,Welter JA,Putzka T,Griep A,Ziegler P,Brandt E,Samer S,Heneka MT,Kaddatz H,Zhan J,Kipp E,Pufe T,Tauber SC,Kipp M,Brandenburg LO

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Fri Apr 24 00:00:00 EDT 2020

Tankyrase inhibition sensitizes melanoma to PD-1 immune checkpoint blockade in syngeneic mouse models.

Communications biology

Waaler J,Mygland L,Tveita A,Strand MF,Solberg NT,Olsen PA,Aizenshtadt A,Fauskanger M,Lund K,Brinch SA,Lycke M,Dybing E,Nygaard V,Bøe SL,Heintz KM,Hovig E,Hammarström C,Corthay A,Krauss S

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Fri Apr 24 00:00:00 EDT 2020

Z-nucleic-acid sensing triggers ZBP1-dependent necroptosis and inflammation.

Nature

Jiao H,Wachsmuth L,Kumari S,Schwarzer R,Lin J,Eren RO,Fisher A,Lane R,Young GR,Kassiotis G,Kaiser WJ,Pasparakis M

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Immunohistochemistry

Wed Apr 01 00:00:00 EDT 2020

Study of Usutu virus neuropathogenicity in mice and human cellular models.

PLoS neglected tropical diseases

Clé M,Barthelemy J,Desmetz C,Foulongne V,Lapeyre L,Bolloré K,Tuaillon E,Erkilic N,Kalatzis V,Lecollinet S,Beck C,Pirot N,Glasson Y,Gosselet F,Alvarez Martinez MT,Van de Perre P,Salinas S,Simonin Y

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Immunohistochemistry

Wed Apr 01 00:00:00 EDT 2020

Therapeutic modulation of phagocytosis in glioblastoma can activate both innate and adaptive antitumour immunity.

Nature communications

von Roemeling CA,Wang Y,Qie Y,Yuan H,Zhao H,Liu X,Yang Z,Yang M,Deng W,Bruno KA,Chan CK,Lee AS,Rosenfeld SS,Yun K,Johnson AJ,Mitchell DA,Jiang W,Kim BYS

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Immunohistochemistry

Fri Mar 20 00:00:00 EDT 2020

Membrane expression of the estrogen receptor ERα is required for intercellular communications in the mammary epithelium.

Development (Cambridge, England)

Gagniac L,Rusidzé M,Boudou F,Cagnet S,Adlanmerini M,Jeannot P,Gaide N,Giton F,Besson A,Weyl A,Gourdy P,Raymond-Letron I,Arnal JF,Brisken C,Lenfant F

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Wed Mar 11 00:00:00 EDT 2020

Modulation of the functions of myeloid-derived suppressor cells : a new strategy of hydrogen sulfide anti-cancer effects.

British journal of pharmacology

De Cicco P,Ercolano G,Rubino V,Terrazzano G,Ruggiero G,Cirino G,Ianaro A

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Sat Feb 01 00:00:00 EST 2020

Atherogenesis in the Carotid Artery with and without Interrupted Blood Flow of Two Hyperlipidemic Mouse Strains.

Journal of vascular research

Zhao J,Huangfu C,Chang Z,Grainger AT,Liu Z,Shi W

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Mon Jan 27 00:00:00 EST 2020

Antitumor and immunomodulatory effects of a novel multitarget inhibitor, CS2164, in mouse hepatocellular carcinoma models.

Anti-cancer drugs

Zhou Y,Fu C,Kong Y,Pan D,Wang Y,Huang S,Li Z,Ning Z,Lu X,Shan S,Xin L

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2019

NLRP3 inflammasome in fibroblasts links tissue damage with inflammation in breast cancer progression and metastasis.

Nature communications

Ershaid N,Sharon Y,Doron H,Raz Y,Shani O,Cohen N,Monteran L,Leider-Trejo L,Ben-Shmuel A,Yassin M,Gerlic M,Ben-Baruch A,Pasmanik-Chor M,Apte R,Erez N

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Thu Sep 26 00:00:00 EDT 2019

FAK activity sustains intrinsic and acquired ovarian cancer resistance to platinum chemotherapy.

eLife

Diaz Osterman CJ,Ozmadenci D,Kleinschmidt EG,Taylor KN,Barrie AM,Jiang S,Bean LM,Sulzmaier FJ,Jean C,Tancioni I,Anderson K,Uryu S,Cordasco EA,Li J,Chen XL,Fu G,Ojalill M,Rappu P,Heino J,Mark AM,Xu G,Fisch KM,Kolev VN,Weaver DT,Pachter JA,Győrffy B,McHale MT,Connolly DC,Molinolo A,Stupack DG,Schlaepfer DD

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Tue Sep 03 00:00:00 EDT 2019

Muscularis Propria Macrophages Alter the Proportion of Nitrergic but Not Cholinergic Gastric Myenteric Neurons.

Cellular and molecular gastroenterology and hepatology

Cipriani G,Terhaar ML,Eisenman ST,Ji S,Linden DR,Wright AM,Sha L,Ordog T,Szurszewski JH,Gibbons SJ,Farrugia G

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Tue Jun 04 00:00:00 EDT 2019

Protumoral bone marrow-derived cells migrate via Gβγ-dependent signaling pathways and exhibit a complex repertoire of RhoGEFs.

Journal of cell communication and signaling

Cervantes-Villagrana RD,Color-Aparicio VM,Reyes-Cruz G,Vázquez-Prado J

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Sat Jun 01 00:00:00 EDT 2019

Dietary calories and lipids synergistically shape adipose tissue cellularity during postnatal growth.

Molecular metabolism

Meln I,Wolff G,Gajek T,Koddebusch J,Lerch S,Harbrecht L,Hong W,Bayindir-Buchhalter I,Krunic D,Augustin HG,Vegiopoulos A

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Sat Jun 01 00:00:00 EDT 2019

Distinct Tie2 tyrosine phosphorylation sites dictate phenotypic switching in endothelial progenitor cells.

Journal of cellular physiology

Siavashi V,Nassiri SM,Rahbarghazi R,Mohseni Z,Sharifi AM

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Wed May 01 00:00:00 EDT 2019

PDGFRα⁺ stromal adipocyte progenitors transition into epithelial cells during lobulo-alveologenesis in the murine mammary gland.

Nature communications

Joshi PA,Waterhouse PD,Kasaian K,Fang H,Gulyaeva O,Sul HS,Boutros PC,Khokha R

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Mon Apr 15 00:00:00 EDT 2019

Growth Factor Screening in Dystrophic Muscles Reveals PDGFB/PDGFRB-Mediated Migration of Interstitial Stem Cells.

International journal of molecular sciences

Camps J,Grosemans H,Gijsbers R,Maes C,Sampaolesi M

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Tue Mar 05 00:00:00 EST 2019

CSF1R antagonism limits local restimulation of antiviral CD8⁺ T cells during viral encephalitis.

Journal of neuroinflammation

Funk KE,Klein RS

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Thu Jan 31 00:00:00 EST 2019

VISTA expression by microglia decreases during inflammation and is differentially regulated in CNS diseases.

Glia

Borggrewe M,Grit C,Den Dunnen WFA,Burm SM,Bajramovic JJ,Noelle RJ,Eggen BJL,Laman JD

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Sat Dec 01 00:00:00 EST 2018

Nur77 variants solely comprising the amino-terminal domain activate hypoxia-inducible factor-1α and affect bone marrow homeostasis in mice and humans.

The Journal of biological chemistry

Koenis DS,Medzikovic L,Vos M,Beldman TJ,van Loenen PB,van Tiel CM,Hamers AAJ,Otermin Rubio I,de Waard V,de Vries CJM

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Immunohistochemistry

Fri Sep 28 00:00:00 EDT 2018

11 Beta-hydroxysteroid dehydrogenase type 1 regulates synovitis, joint destruction, and systemic bone loss in chronic polyarthritis.

Journal of autoimmunity

Hardy RS,Fenton C,Croft AP,Naylor AJ,Begum R,Desanti G,Buckley CD,Lavery G,Cooper MS,Raza K

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Wed Aug 01 00:00:00 EDT 2018

Endothelial cells in the innate response to allergens and initiation of atopic asthma.

The Journal of clinical investigation

Asosingh K,Weiss K,Queisser K,Wanner N,Yin M,Aronica M,Erzurum S

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Mon Jul 02 00:00:00 EDT 2018

Endothelial cells in the innate response to allergens and initiation of atopic asthma.

The Journal of clinical investigation

Asosingh K,Weiss K,Queisser K,Wanner N,Yin M,Aronica M,Erzurum S

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Mon Jul 02 00:00:00 EDT 2018

Mitophagy in Intestinal Epithelial Cells Triggers Adaptive Immunity during Tumorigenesis.

Cell

Ziegler PK,Bollrath J,Pallangyo CK,Matsutani T,Canli Ö,De Oliveira T,Diamanti MA,Müller N,Gamrekelashvili J,Putoczki T,Horst D,Mankan AK,Öner MG,Müller S,Müller-Höcker J,Kirchner T,Slotta-Huspenina J,Taketo MM,Reinheckel T,Dröse S,Larner AC,Wels WS,Ernst M,Greten TF,Arkan MC,Korn T,Wirth D,Greten FR

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Thu Jun 28 00:00:00 EDT 2018

mTOR Modulates CD8+ T Cell Differentiation in Mice with Invasive Pulmonary Aspergillosis.

Open life sciences

Wang H,Xiao Y,Su L,Cui N,Liu D

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Mon Jan 01 00:00:00 EST 2018

Dimethyl fumarate treatment after traumatic brain injury prevents depletion of antioxidative brain glutathione and confers neuroprotection.

Journal of neurochemistry

Krämer T,Grob T,Menzel L,Hirnet T,Griemert E,Radyushkin K,Thal SC,Methner A,Schaefer MKE

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Immunohistochemistry

Fri Dec 01 00:00:00 EST 2017

β3-Adrenergic Regulation of EPC Features Through Manipulation of the Bone Marrow MSC Niche.

Journal of cellular biochemistry

Vafaei R,Nassiri SM,Siavashi V

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Fri Dec 01 00:00:00 EST 2017

ECM-Dependence of Endothelial Progenitor Cell Features.

Journal of cellular biochemistry

Siavashi V,Nassiri SM,Rahbarghazi R,Vafaei R,Sariri R

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2016

The Nuclear Orphan Receptor NR2F6 Is a Central Checkpoint for Cancer Immune Surveillance.

Cell reports

Hermann-Kleiter N,Klepsch V,Wallner S,Siegmund K,Klepsch S,Tuzlak S,Villunger A,Kaminski S,Pfeifhofer-Obermair C,Gruber T,Wolf D,Baier G

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Tue Sep 29 00:00:00 EDT 2015

Bone marrow mesenchymal stem cells provide an antibiotic-protective niche for persistent viable Mycobacterium tuberculosis that survive antibiotic treatment.

The American journal of pathology

Beamer G,Major S,Das B,Campos-Neto A

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Mon Dec 01 00:00:00 EST 2014

Reprogramming human endothelial cells to haematopoietic cells requires vascular induction.

Nature

Sandler VM,Lis R,Liu Y,Kedem A,James D,Elemento O,Butler JM,Scandura JM,Rafii S

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Thu Jul 17 00:00:00 EDT 2014

The nucleotide-binding leucine-rich repeat (NLR) family member NLRX1 mediates protection against experimental autoimmune encephalomyelitis and represses macrophage/microglia-induced inflammation.

The Journal of biological chemistry

Eitas TK,Chou WC,Wen H,Gris D,Robbins GR,Brickey J,Oyama Y,Ting JP

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Fri Feb 14 00:00:00 EST 2014

Gli1 deletion prevents Helicobacter-induced gastric metaplasia and expansion of myeloid cell subsets.

PloS one

El-Zaatari M,Kao JY,Tessier A,Bai L,Hayes MM,Fontaine C,Eaton KA,Merchant JL

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Tue Sep 10 00:00:00 EDT 2013

Effect of topical azithromycin on corneal innate immune responses.

Investigative ophthalmology & visual science

Sadrai Z,Hajrasouliha AR,Chauhan S,Saban DR,Dastjerdi MH,Dana R

Published figure using CD45 monoclonal antibody (Product # 78-0451-82) in Flow Cytometry

Tue Apr 19 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: CD45; L-CA; Leukocyte common antigen; Ly-5; Lymphocyte antigen 5; lymphocyte common antigen; Receptor-type tyrosine-protein phosphatase C; T200; T220 and B220

Gene Aliases: B220; Cd45; CD45R; L-CA; loc; Ly-5; Lyt-4; Ptprc; T200

UniProt ID: (Mouse) P06800

Entrez Gene ID: (Mouse) 19264

Molecular Function: protein phosphatase protein modifying enzyme

Function(s)

Process(es)

phosphoprotein phosphatase activity protein tyrosine phosphatase activity protein binding heparin binding hydrolase activity phosphatase activity protein kinase binding heparan sulfate proteoglycan binding

CD45 Monoclonal Antibody (30-F11), Super Bright™ 780, eBioscience™

Product Details

78-0451-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!