|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Storage buffer||PBS with sucrose, 4mg/ml BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Flow Cytometry (Flow)||See 4 publications below|
PerCP conjugates are excited by the 488 nm line of the argon-ion laser and have a large Stokes shift, providing minimal spectral overlap into the R-PE (phycoerythrin) channel.
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells.
Analyte Specific Reagent
CD40 Expression in Fibrocytes Is Induced by TSH: Potential Synergistic Immune Activation.
MHCD4531 was used in flow cytometry discuss the potential roles of TSHR and CD40 in thyroid-associated ophthalmopathy
|Mester T,Raychaudhuri N,Gillespie EF,Chen H,Smith TJ,Douglas RS||PloS one (11:null)||2016|
Thyrotropin receptor and CD40 mediate interleukin-8 expression in fibrocytes: implications for thyroid-associated ophthalmopathy (an American Ophthalmological Society thesis).
MHCD4531 was used in flow cytometry to characterize the thyrotropin receptor, CD40, and IL-8 in peripheral blood fibrocytes of patients with thyroid-associated orbitopathy
|Douglas RS,Mester T,Ginter A,Kim DS||Transactions of the American Ophthalmological Society (112:26)||2014|
Teprotumumab, an IGF-1R blocking monoclonal antibody inhibits TSH and IGF-1 action in fibrocytes.
MHCD4531 was used in flow cytometry to describe the phase II trial using teprotumumab (RV 001, R1507) to treat patients with active thyroid-associated ophthalmopathy
|Chen H,Mester T,Raychaudhuri N,Kauh CY,Gupta S,Smith TJ,Douglas RS||The Journal of clinical endocrinology and metabolism (99:E1635)||2014|
Accurate quantitation of D+ fetomaternal hemorrhage by flow cytometry using a novel reagent to eliminate granulocytes from analysis.
MHCD4531 was used in flow cytometry to discuss methods to detect fetomaternal hemorrhage.
|Kumpel B,Hazell M,Guest A,Dixey J,Mushens R,Bishop D,Wreford-Bush T,Lee E||Transfusion (54:1305)||2014|