Invitrogen

CD45 Monoclonal Antibody (HI30), FITC, eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

39 Published Figures

40 References

View all (311) CD45 antibodies

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CD45 Antibody in Flow Cytometry (Flow) — Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (blue histogram) or Anti-Human CD45 FITC (purple histogram). Cells in the lymphocyte gate were used for analysis.

CD45 Antibody — Figure 8 PRMT5 inhibition reduces long-term multilineage engraftment of human CML CD34 + cells in NSI mice. ( A ) Human CML CD34 + cells were treated with PJ-68 (25.0 muM) in vitro for 3 days and injected into sublethally irradiated (300 cGy) NSI mice. After 12 weeks, human multilineage engraftment was analyzed by flow cytometry. ( B and C ) The percentage ( B ) and the absolute number ( C ) of human CD45 + cells engrafted in the BM after transplantation of human CML CD34 + cells (1 x 10 6 cells/mouse) for 12 weeks. ( D ) The proportion of human CD45 + cells engrafted in the spleen at 12 weeks. ( E ) qRT-PCR analysis of BCR-ABL mRNA level in CD45 + cells engrafted in BM at 12 weeks. ( F ) The proportion of engraftment of human CD33, CD34, CD11B, CD14, and CD19 in BM. ( G ) Representative flow cytometry plots of human CD45 and CD33 expression in mice with cells from 1 of the 2 CML patients ( n = 3 for control, n = 4 for PJ-68 treatment, each patient). * P < 0.05, ** P < 0.01, *** P < 0.0001, 2-tailed Student's t test. {TM}

CD45 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow) — Figure 1 Detection of cells with abnormal nuclei using imaging flow cytometry. ( a ) Representative cell images with the same DAPI intensity. CD45, a lymphocyte biomarker, was labeled with PE-Cy5. Upper panel: CD45 + lymphocyte with a smaller DAPI area. Lower panel: CD45 - cells with a larger DAPI area, showing a looser structure of nuclei. Scale bars represent 20 mum. ( b ) Mean DAPI fluorescence intensity in PBMCs. Unlike the cells with a normal DAPI area (lower panel), a G1 and G2 peak could be detected in the cells with larger DAPI areas (upper panel). ( c ) Imaging flow cytometry test results for the peripheral blood samples from MVI patients. Left panel, basic results for peripheral blood mononuclear cells (PBMCs), the horizontal axis indicates the cell area and the vertical axis indicates the aspect ratio. The gate displays the group of single cells. Right panel: patients with MVI had 68.1 +- 14.8 cells with large DAPI areas. ( d ) Representative images for PBMCs from HCC patients. CD45, a lymphocyte biomarker, was labeled with PE-Cy5. EpCAM, a biomarker of circulating tumor cells (CTCs), was labeled with FITC. Left panel: cells with high karyoplasmic ratios (HKR cells), which were EpCAM-positive and CD45-negative. Right panel: cells with normal karyoplasmic ratios (normal cells), which were EpCAM-negative and CD45-positive. Scale bars represent 20 mum. ( e ) Flow cytometry test results for HKR cells from MVI patients. All HKR cells were CD45 - , but only 8.7% of HKR c

CD45 Antibody in Immunocytochemistry (ICC/IF) — Figure 2 Separation of CTCs and leukocyte or endothelial cells. ( A ) Technical concept using CTC-FIND for single cell isolation. ( B ) Scheme of cell classification. ( C - E ) Representative fluorescence image of CTCs detected in clinical blood specimens. The blood samples (8 mL) collected from patients with mCRC were evaluated by fluorescence microscopy after separation to single cells by CTC-FIND. Specimens were enriched and stained with Hoechst 33342 (pseudo colored blue) and negative markers composed of anti-CD45 and anit-CD50 antibodies (pseudo colored red), followed by the acquisition of fluorescence images for each marker and digital overlay of the two images (merge). Hoechst 33342-positive and CD45/50-negative CTCs were counted and collected from each specimen. Gallery of CTCs ( C ), CTC clusters ( D ) and leucocytes ( E ). Original magnification, x500.

CD45 Antibody in Immunohistochemistry (IHC) — Fig. 4 Silence of Usp47 inhibits BCR-ABL T315I -induced CML and inhibits proliferation of KBM T315I in a xenograft model. a Survival of mice after the transplantation of BCR-ABL T315I -transduced Usp47 +/+ or Usp47 -/- BM cells ( n = 6 biologically independent samples per group). **** p < 0.0001, p -value was analyzed by Mantel-Cox-log-rank test. The experiment was repeated three times. b , c Size ( b ) and weight ( c ) of the spleen in the two groups of mice ( n = 5 biologically independent samples per group) at 28 days after transplantation. Center lines of the box and whisker plot represent median values, whereas the box edges indicate the 25th and 75th centiles, and the whiskers indicate the minimum and maximum values. d , e At 28 days after transplantation, the morphology of cells from PB ( d ) was examined by Wright-Giemsa staining; the percentages of GFP + and Gr-1 + cells ( e ) in BM were examined by FACS analysis. f H&E staining of the liver and spleen at 28 days after transplantation. Scale bars, 50 mum. g Ctrl shRNA or ShUSP47-2 transfected KBM5 T315I cells ( n = 8 biologically independent samples per group) were injected into B-NDG mice through the tail vein. **** p < 0.0001, p- value was analyzed by Mantel-Cox-log-rank test. h Size and weight of spleen at day 21 ( n = 6 biologically independent samples per group). Center lines of the box and whisker plot represent median values, whereas the box edges indicate the 25th and 75th centiles, and the whiskers indicate

CD45 Antibody in Western Blot (WB) — Published figure using CD45 monoclonal antibody (Product # 11-0459-42) in Western Blot

Product Details

11-0459-42

Applications	Tested Dilution	Publications
Western Blot (WB)	-	View 2 publications 2 publications
Immunohistochemistry (IHC)	-	View 3 publications 3 publications
Immunocytochemistry (ICC/IF)	-	View 5 publications 5 publications
Flow Cytometry (Flow)	5 µL (0.25 µg)/test	View 30 publications 30 publications

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Mouse / IgG1, kappa
Recommended Isotype Control	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	HI30
Conjugate	FITC Alexa Fluor 532 Alexa Fluor 561 Alexa Fluor 647 Alexa Fluor 660 APC APC-eFluor 780 Biotin Brilliant UV 395 Brilliant UV 496 Brilliant UV 737 Brilliant UV 805 eFluor 450 eFluor 506 NovaFluor Blue 510 NovaFluor Blue 585 NovaFluor Red 710 NovaFluor Yellow 590 NovaFluor Yellow 660 NovaFluor Yellow 730 PE PE-Cyanine5 PE-Cyanine5.5 PE-Cyanine7 PE-eFluor 610 PerCP-Cyanine5.5
Excitation/Emission Profile	View spectra
Form	Liquid
Concentration	5 µL/Test
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2, with 0.1% gelatin, 0.2% BSA
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_10852703

Product Specific Information

Description: The HI30 monoclonal antibody reacts with all isoforms of human CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.

Applications Reported: The HI30 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HI30 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

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Product References

Clinical Significance of Circulating Tumor Cell Induced Epithelial-Mesenchymal Transition in Patients with Metastatic Colorectal Cancer by Single-Cell RNA-Sequencing.

Cancers

Kozuka M,Battaglin F,Jayachandran P,Wang J,Arai H,Soni S,Zhang W,Hirai M,Matsusaka S,Lenz HJ

Published figure using CD45 monoclonal antibody (Product # 11-0459-42) in Immunocytochemistry

Tue Sep 28 00:00:00 EDT 2021

Direct Comparative Analyses of 10X Genomics Chromium and Smart-seq2.

Genomics, proteomics & bioinformatics

Wang X,He Y,Zhang Q,Ren X,Zhang Z

11-0459-42 was used in Flow Cytometry to promote better understanding of these two platforms and offers the basis for an informed choice of these widely used technologies.

Thu Apr 01 00:00:00 EDT 2021

Targeting USP47 overcomes tyrosine kinase inhibitor resistance and eradicates leukemia stem/progenitor cells in chronic myelogenous leukemia.

Nature communications

Lei H,Xu HZ,Shan HZ,Liu M,Lu Y,Fang ZX,Jin J,Jing B,Xiao XH,Gao SM,Gao FH,Xia L,Yang L,Liu LG,Wang WW,Liu CX,Tong Y,Wu YZ,Zheng JK,Chen GQ,Zhou L,Wu YL

Published figure using CD45 monoclonal antibody (Product # 11-0459-42) in Immunohistochemistry

Mon Jan 04 00:00:00 EST 2021

Intratumoral FoxP3⁺Helios⁺ Regulatory T Cells Upregulating Immunosuppressive Molecules Are Expanded in Human Colorectal Cancer.

Frontiers in immunology

Syed Khaja AS,Toor SM,El Salhat H,Ali BR,Elkord E

11-0459 was used in Flow cytometry/Cell sorting to characterise the differences in immune cells in the tumour microenvironment of colorectal cancer patients compared to paired adjacent non-tumour colon tissue of CRC patients.

Sun Jan 03 00:00:00 EST 2021

Emergence of a High-Plasticity Cell State during Lung Cancer Evolution.

Cancer cell

Marjanovic ND,Hofree M,Chan JE,Canner D,Wu K,Trakala M,Hartmann GG,Smith OC,Kim JY,Evans KV,Hudson A,Ashenberg O,Porter CBM,Bejnood A,Subramanian A,Pitter K,Yan Y,Delorey T,Phillips DR,Shah N,Chaudhary O,Tsankov A,Hollmann T,Rekhtman N,Massion PP,Poirier JT,Mazutis L,Li R,Lee JH,Amon A,Rudin CM,Jacks T,Regev A,Tammela T

11-0459-42 was used in Flow Cytometry to profile cellular transcriptomes of lung tumours at different stages.

Mon Aug 10 00:00:00 EDT 2020

A Human Skeletal Muscle Atlas Identifies the Trajectories of Stem and Progenitor Cells across Development and from Human Pluripotent Stem Cells.

Cell stem cell

Xi H,Langerman J,Sabri S,Chien P,Young CS,Younesi S,Hicks M,Gonzalez K,Fujiwara W,Marzi J,Liebscher S,Spencer M,Van Handel B,Evseenko D,Schenke-Layland K,Plath K,Pyle AD

11-0459-42 was used in Flow Cytometry to profile human embryonic, fetal and postnatal skeletal muscle tissues to develop a roadmap of muscle ontogeny.

Thu Jul 02 00:00:00 EDT 2020

Single-Cell Analyses Inform Mechanisms of Myeloid-Targeted Therapies in Colon Cancer.

Cell

Zhang L,Li Z,Skrzypczynska KM,Fang Q,Zhang W,O'Brien SA,He Y,Wang L,Zhang Q,Kim A,Gao R,Orf J,Wang T,Sawant D,Kang J,Bhatt D,Lu D,Li CM,Rapaport AS,Perez K,Ye Y,Wang S,Hu X,Ren X,Ouyang W,Shen Z,Egen JG,Zhang Z,Yu X

11-0459-42 was used in Flow Cytometry to identify critical cellular interactions regulating tumor immunity and defines mechanisms underlying myeloid-targeted immunotherapies currently undergoing clinical testing.

Thu Apr 16 00:00:00 EDT 2020

Side-by-side comparison of BH3-mimetics identifies MCL-1 as a key therapeutic target in AML.

Cell death & disease

Ewald L,Dittmann J,Vogler M,Fulda S

Published figure using CD45 monoclonal antibody (Product # 11-0459-42) in Flow Cytometry

Wed Dec 04 00:00:00 EST 2019

Mechanisms of virus dissemination in bone marrow of HIV-1-infected humanized BLT mice.

eLife

Ladinsky MS,Khamaikawin W,Jung Y,Lin S,Lam J,An DS,Bjorkman PJ,Kieffer C

Published figure using CD45 monoclonal antibody (Product # 11-0459-42) in Flow Cytometry

Mon Oct 28 00:00:00 EDT 2019

Human blood vessel organoids as a model of diabetic vasculopathy.

Nature

Wimmer RA,Leopoldi A,Aichinger M,Wick N,Hantusch B,Novatchkova M,Taubenschmid J,Hämmerle M,Esk C,Bagley JA,Lindenhofer D,Chen G,Boehm M,Agu CA,Yang F,Fu B,Zuber J,Knoblich JA,Kerjaschki D,Penninger JM

11-0459 was used in Flow cytometry/Cell sorting to suggest that blood vessel organoids derived from human stem cells faithfully recapitulate the structure and function of human blood vessels and are a suitable model for diabetic vasculopathy.

Tue Jan 01 00:00:00 EST 2019

Human umbilical cord-derived mesenchymal stem cells ameliorate the enteropathy of food allergies in mice.

Experimental and therapeutic medicine

Yan N,Xu J,Zhao C,Wu Y,Gao F,Li C,Zhou W,Xiao T,Zhou X,Shao Q,Xia S

Published figure using CD45 monoclonal antibody (Product # 11-0459-42) in Flow Cytometry

Sat Dec 01 00:00:00 EST 2018

Single nucleotide variant profiles of viable single circulating tumour cells reveal CTC behaviours in breast cancer.

Oncology reports

Wang Y,Guo L,Feng L,Zhang W,Xiao T,Di X,Chen G,Zhang K

Published figure using CD45 monoclonal antibody (Product # 11-0459-42) in Flow Cytometry

Tue May 01 00:00:00 EDT 2018

Evaluation of a simple method for storage of blood samples that enables isolation of circulating tumor cells 96 h after sample collection.

Journal of biological research (Thessalonike, Greece)

Apostolou P,Ntanovasilis DA,Papasotiriou I

Published figure using CD45 monoclonal antibody (Product # 11-0459-42) in Immunofluorescence

Fri Dec 01 00:00:00 EST 2017

Evaluation of a simple method for storage of blood samples that enables isolation of circulating tumor cells 96 h after sample collection.

Journal of biological research (Thessalonike, Greece)

Apostolou P,Ntanovasilis DA,Papasotiriou I

Published figure using CD45 monoclonal antibody (Product # 11-0459-42) in Immunofluorescence

Fri Dec 01 00:00:00 EST 2017

Targeted Disruption of TCF12 Reveals HEB as Essential in Human Mesodermal Specification and Hematopoiesis.

Stem cell reports

Li Y,Brauer PM,Singh J,Xhiku S,Yoganathan K,Zúñiga-Pflücker JC,Anderson MK

Published figure using CD45 monoclonal antibody (Product # 11-0459-42) in Flow Cytometry

Tue Sep 12 00:00:00 EDT 2017

Enhancement of cytokine-driven NK cell IFN-γ production after vaccination of HCMV infected Africans.

European journal of immunology

Darboe A,Danso E,Clarke E,Umesi A,Touray E,Wegmuller R,Moore SE,Riley EM,Goodier MR

11-0459 was used in Flow cytometry/Cell sorting to investigate the impact of advanced NK cell differentiation on vaccine-induced responses.

Thu Jun 01 00:00:00 EDT 2017

Preferential accumulation of regulatory T cells with highly immunosuppressive characteristics in breast tumor microenvironment.

Oncotarget

Syed Khaja AS,Toor SM,El Salhat H,Faour I,Ul Haq N,Ali BR,Elkord E

Tue May 16 00:00:00 EDT 2017

Guanine-Rich Sequences Are a Dominant Feature of Exosomal microRNAs across the Mammalian Species and Cell Types.

PloS one

Momose F,Seo N,Akahori Y,Sawada S,Harada N,Ogura T,Akiyoshi K,Shiku H

11-0459 was used in Flow cytometry/Cell sorting to statistically analyse miRNA microarray data, revealing that guanine-rich sequences are a dominant feature of exosome-dominant miRNAs.

Wed Apr 05 00:00:00 EDT 2017

Human umbilical cord mesenchymal stem cells improve the reserve function of perimenopausal ovary via a paracrine mechanism.

Stem cell research & therapy

Li J,Mao Q,He J,She H,Zhang Z,Yin C

Published figure using CD45 monoclonal antibody (Product # 11-0459-42) in Flow Cytometry

Thu Mar 09 00:00:00 EST 2017

Circulating tumor cell detection in hepatocellular carcinoma based on karyoplasmic ratios using imaging flow cytometry.

Scientific reports

Liu Z,Guo W,Zhang D,Pang Y,Shi J,Wan S,Cheng K,Wang J,Cheng S

Published figure using CD45 monoclonal antibody (Product # 11-0459-42) in Flow Cytometry

Fri Dec 23 00:00:00 EST 2016

Circulating tumor cell detection in hepatocellular carcinoma based on karyoplasmic ratios using imaging flow cytometry.

Scientific reports

Liu Z,Guo W,Zhang D,Pang Y,Shi J,Wan S,Cheng K,Wang J,Cheng S

Published figure using CD45 monoclonal antibody (Product # 11-0459-42) in Flow Cytometry

Fri Dec 23 00:00:00 EST 2016

Targeting methyltransferase PRMT5 eliminates leukemia stem cells in chronic myelogenous leukemia.

The Journal of clinical investigation

Jin Y,Zhou J,Xu F,Jin B,Cui L,Wang Y,Du X,Li J,Li P,Ren R,Pan J

Published figure using CD45 monoclonal antibody (Product # 11-0459-42) in Flow Cytometry

Mon Oct 03 00:00:00 EDT 2016

Kinase-independent function of RIP1, critical for mature T-cell survival and proliferation.

Cell death & disease

Dowling JP,Cai Y,Bertin J,Gough PJ,Zhang J

Published figure using CD45 monoclonal antibody (Product # 11-0459-42) in Flow Cytometry

Thu Sep 29 00:00:00 EDT 2016

Platelets confound the measurement of extracellular miRNA in archived plasma.

Scientific reports

Mitchell AJ,Gray WD,Hayek SS,Ko YA,Thomas S,Rooney K,Awad M,Roback JD,Quyyumi A,Searles CD

Published figure using CD45 monoclonal antibody (Product # 11-0459-42) in Flow Cytometry

Tue Sep 13 00:00:00 EDT 2016

A 3D culture system enhances the ability of human bone marrow stromal cells to support the growth of limbal stem/progenitor cells.

Stem cell research

González S,Mei H,Nakatsu MN,Baclagon ER,Deng SX

Published figure using CD45 monoclonal antibody (Product # 11-0459-42) in Immunocytochemistry

Tue Mar 01 00:00:00 EST 2016

UTX inhibition as selective epigenetic therapy against TAL1-driven T-cell acute lymphoblastic leukemia.

Genes & development

Benyoucef A,Palii CG,Wang C,Porter CJ,Chu A,Dai F,Tremblay V,Rakopoulos P,Singh K,Huang S,Pflumio F,Hébert J,Couture JF,Perkins TJ,Ge K,Dilworth FJ,Brand M

Published figure using CD45 monoclonal antibody (Product # 11-0459-42) in Flow Cytometry

Tue Mar 01 00:00:00 EST 2016

Inactivation of nuclear GSK3β by Ser(389) phosphorylation promotes lymphocyte fitness during DNA double-strand break response.

Nature communications

Thornton TM,Delgado P,Chen L,Salas B,Krementsov D,Fernandez M,Vernia S,Davis RJ,Heimann R,Teuscher C,Krangel MS,Ramiro AR,Rincón M

Published figure using CD45 monoclonal antibody (Product # 11-0459-42) in Western Blot

Fri Jan 29 00:00:00 EST 2016

Human esophageal myofibroblasts secrete proinflammatory cytokines in response to acid and Toll-like receptor 4 ligands.

American journal of physiology. Gastrointestinal and liver physiology

Gargus M,Niu C,Vallone JG,Binkley J,Rubin DC,Shaker A

Published figure using CD45 monoclonal antibody (Product # 11-0459-42) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2015

A novel function of RIP1 in postnatal development and immune homeostasis by protecting against RIP3-dependent necroptosis and FADD-mediated apoptosis.

Frontiers in cell and developmental biology

Dowling JP,Nair A,Zhang J

Published figure using CD45 monoclonal antibody (Product # 11-0459-42) in Western Blot

Fri Mar 13 00:00:00 EDT 2015

Retinoic acid regulates hematopoietic development from human pluripotent stem cells.

Stem cell reports

Rönn RE,Guibentif C,Moraghebi R,Chaves P,Saxena S,Garcia B,Woods NB

Published figure using CD45 monoclonal antibody (Product # 11-0459-42) in Flow Cytometry

Tue Feb 10 00:00:00 EST 2015

A Small Molecule β2 Integrin Agonist Improves Chronic Kidney Allograft Survival by Reducing Leukocyte Recruitment and Accompanying Vasculopathy.

Frontiers in medicine

Khan SQ,Guo L,Cimbaluk DJ,Elshabrawy H,Faridi MH,Jolly M,George JF,Agarwal A,Gupta V

Published figure using CD45 monoclonal antibody (Product # 11-0459-42) in Immunofluorescence

Fri Jan 16 00:00:00 EST 2015

Hypoxia-cultured human adipose-derived mesenchymal stem cells are non-oncogenic and have enhanced viability, motility, and tropism to brain cancer.

Cell death & disease

Feng Y,Zhu M,Dangelmajer S,Lee YM,Wijesekera O,Castellanos CX,Denduluri A,Chaichana KL,Li Q,Zhang H,Levchenko A,Guerrero-Cazares H,Quiñones-Hinojosa A

Thu Dec 11 00:00:00 EST 2014

Caveolin-1 regulates chemokine receptor 5-mediated contribution of bone marrow-derived cells to dermal fibrosis.

Frontiers in pharmacology

Lee R,Perry B,Heywood J,Reese C,Bonner M,Hatfield CM,Silver RM,Visconti RP,Hoffman S,Tourkina E

Published figure using CD45 monoclonal antibody (Product # 11-0459-42) in Immunofluorescence

Thu Jun 26 00:00:00 EDT 2014

Caveolin-1 regulates chemokine receptor 5-mediated contribution of bone marrow-derived cells to dermal fibrosis.

Frontiers in pharmacology

Lee R,Perry B,Heywood J,Reese C,Bonner M,Hatfield CM,Silver RM,Visconti RP,Hoffman S,Tourkina E

Published figure using CD45 monoclonal antibody (Product # 11-0459-42) in Immunofluorescence

Thu Jun 26 00:00:00 EDT 2014

Microvesicles derived from human umbilical cord Wharton's jelly mesenchymal stem cells attenuate bladder tumor cell growth in vitro and in vivo.

PloS one

Wu S,Ju GQ,Du T,Zhu YJ,Liu GH

Published figure using CD45 monoclonal antibody (Product # 11-0459-42) in Flow Cytometry

Tue Oct 22 00:00:00 EDT 2013

Plasmacytoid dendritic cells are scarcely represented in the human gut mucosa and are not recruited to the celiac lesion.

Mucosal immunology

Ráki M,Beitnes AC,Lundin KE,Jahnsen J,Jahnsen FL,Sollid LM

11-0459 was used in Flow cytometry/Cell sorting to suggest that IFN-α is present in the celiac mucosa and IFN-λ is important in intestinal antiviral defense.

Sun Sep 01 00:00:00 EDT 2013

Gliomas promote immunosuppression through induction of B7-H1 expression in tumor-associated macrophages.

Clinical cancer research : an official journal of the American Association for Cancer Research

Bloch O,Crane CA,Kaur R,Safaee M,Rutkowski MJ,Parsa AT

11-0459 was used in Flow cytometry/Cell sorting to demonstrate that gliomas can upregulate B7-H1 expression in circulating monocytes and tumor-infiltrative macrophages through modulation of autocrine/paracrine IL-10 signaling.

Sat Jun 15 00:00:00 EDT 2013

Identification of a candidate proteomic signature to discriminate multipotent and non-multipotent stromal cells.

PloS one

Rosu-Myles M,She YM,Fair J,Muradia G,Mehic J,Menendez P,Prasad SS,Cyr TD

11-0459 was used in Flow cytometry/Cell sorting to provide evidence of heterogeneity among bone marrow stromal cells and identify potential candidate proteins for quantifying MSCs in vitro.

Thu Dec 13 00:00:00 EST 2012

Rapid accumulation of CD14+CD11c+ dendritic cells in gut mucosa of celiac disease after in vivo gluten challenge.

PloS one

Beitnes AC,Ráki M,Brottveit M,Lundin KE,Jahnsen FL,Sollid LM

11-0459 was used in Flow cytometry/Cell sorting to study the varying dendritic cell subset responses within the celiac disease lesion using a model of in vivo gluten challenge.

Mon Aug 13 00:00:00 EDT 2012

Yield and characterization of subcutaneous human adipose-derived stem cells by flow cytometric and adipogenic mRNA analyzes.

Cytotherapy

Yu G,Wu X,Dietrich MA,Polk P,Scott LK,Ptitsyn AA,Gimble JM

Published figure using CD45 monoclonal antibody (Product # 11-0459-42) in Flow Cytometry

Thu Jul 01 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: CD45; CD45 antigen; L-CA; Leukocyte common antigen; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen; T220 and B220

Gene Aliases: B220; CD45; CD45R; GP180; L-CA; LCA; LY5; PTPRC; T200

UniProt ID: (Human) P08575

Entrez Gene ID: (Human) 5788

Molecular Function: protein phosphatase protein modifying enzyme