Invitrogen

CD45 Monoclonal Antibody (HI30), eFluor™ 450, eBioscience™

Product Image Gallery

CD45 Antibody in Flow Cytometry (Flow) — Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control eFluor® 450 (Product # 48-4714-82) (blue histogram) or Anti-Human CD45 eFluor® 450 (purple histogram). Cells in the lymphocyte gate were used for analysis.

CD45 Antibody in Immunocytochemistry (ICC/IF) — Figure 2 Separation of CTCs and leukocyte or endothelial cells. ( A ) Technical concept using CTC-FIND for single cell isolation. ( B ) Scheme of cell classification. ( C - E ) Representative fluorescence image of CTCs detected in clinical blood specimens. The blood samples (8 mL) collected from patients with mCRC were evaluated by fluorescence microscopy after separation to single cells by CTC-FIND. Specimens were enriched and stained with Hoechst 33342 (pseudo colored blue) and negative markers composed of anti-CD45 and anit-CD50 antibodies (pseudo colored red), followed by the acquisition of fluorescence images for each marker and digital overlay of the two images (merge). Hoechst 33342-positive and CD45/50-negative CTCs were counted and collected from each specimen. Gallery of CTCs ( C ), CTC clusters ( D ) and leucocytes ( E ). Original magnification, x500.

CD45 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow) — Figure 1 Detection of cells with abnormal nuclei using imaging flow cytometry. ( a ) Representative cell images with the same DAPI intensity. CD45, a lymphocyte biomarker, was labeled with PE-Cy5. Upper panel: CD45 + lymphocyte with a smaller DAPI area. Lower panel: CD45 - cells with a larger DAPI area, showing a looser structure of nuclei. Scale bars represent 20 mum. ( b ) Mean DAPI fluorescence intensity in PBMCs. Unlike the cells with a normal DAPI area (lower panel), a G1 and G2 peak could be detected in the cells with larger DAPI areas (upper panel). ( c ) Imaging flow cytometry test results for the peripheral blood samples from MVI patients. Left panel, basic results for peripheral blood mononuclear cells (PBMCs), the horizontal axis indicates the cell area and the vertical axis indicates the aspect ratio. The gate displays the group of single cells. Right panel: patients with MVI had 68.1 +- 14.8 cells with large DAPI areas. ( d ) Representative images for PBMCs from HCC patients. CD45, a lymphocyte biomarker, was labeled with PE-Cy5. EpCAM, a biomarker of circulating tumor cells (CTCs), was labeled with FITC. Left panel: cells with high karyoplasmic ratios (HKR cells), which were EpCAM-positive and CD45-negative. Right panel: cells with normal karyoplasmic ratios (normal cells), which were EpCAM-negative and CD45-positive. Scale bars represent 20 mum. ( e ) Flow cytometry test results for HKR cells from MVI patients. All HKR cells were CD45 - , but only 8.7% of HKR c

CD45 Antibody in Immunohistochemistry (IHC) — Fig. 4 Silence of Usp47 inhibits BCR-ABL T315I -induced CML and inhibits proliferation of KBM T315I in a xenograft model. a Survival of mice after the transplantation of BCR-ABL T315I -transduced Usp47 +/+ or Usp47 -/- BM cells ( n = 6 biologically independent samples per group). **** p < 0.0001, p -value was analyzed by Mantel-Cox-log-rank test. The experiment was repeated three times. b , c Size ( b ) and weight ( c ) of the spleen in the two groups of mice ( n = 5 biologically independent samples per group) at 28 days after transplantation. Center lines of the box and whisker plot represent median values, whereas the box edges indicate the 25th and 75th centiles, and the whiskers indicate the minimum and maximum values. d , e At 28 days after transplantation, the morphology of cells from PB ( d ) was examined by Wright-Giemsa staining; the percentages of GFP + and Gr-1 + cells ( e ) in BM were examined by FACS analysis. f H&E staining of the liver and spleen at 28 days after transplantation. Scale bars, 50 mum. g Ctrl shRNA or ShUSP47-2 transfected KBM5 T315I cells ( n = 8 biologically independent samples per group) were injected into B-NDG mice through the tail vein. **** p < 0.0001, p- value was analyzed by Mantel-Cox-log-rank test. h Size and weight of spleen at day 21 ( n = 6 biologically independent samples per group). Center lines of the box and whisker plot represent median values, whereas the box edges indicate the 25th and 75th centiles, and the whiskers indicate

Product Details

48-0459-42

Applications	Tested Dilution	Publications
Immunohistochemistry (IHC)	-	View 3 publications 3 publications
Immunocytochemistry (ICC/IF)	-	View 5 publications 5 publications
Flow Cytometry (Flow)	5 µL (0.5 µg)/test	View 26 publications 26 publications

Product Specifications
Species Reactivity	Human
Published species	Human, Mouse
Host/Isotype	Mouse / IgG1, kappa
Recommended Isotype Control	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 450, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	HI30
Conjugate	eFluor™ 450 Alexa Fluor 532 Alexa Fluor 561 Alexa Fluor 647 Alexa Fluor 660 APC APC-eFluor 780 Biotin Brilliant UV 395 Brilliant UV 496 Brilliant UV 737 Brilliant UV 805 eFluor 506 FITC NovaFluor Blue 510 NovaFluor Blue 585 NovaFluor Red 710 NovaFluor Yellow 590 NovaFluor Yellow 660 NovaFluor Yellow 730 PE PE-Cyanine5 PE-Cyanine5.5 PE-Cyanine7 PE-eFluor 610 PerCP-Cyanine5.5
Excitation/Emission Profile	View spectra
Form	Liquid
Concentration	5 µL/Test
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2, with 0.1% gelatin, 0.2% BSA
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_2016677

Product Specific Information

Description: The HI30 monoclonal antibody reacts with all isoforms of human CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.

Applications Reported: This HI30 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HI30 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Clinical Significance of Circulating Tumor Cell Induced Epithelial-Mesenchymal Transition in Patients with Metastatic Colorectal Cancer by Single-Cell RNA-Sequencing.

Cancers

Kozuka M,Battaglin F,Jayachandran P,Wang J,Arai H,Soni S,Zhang W,Hirai M,Matsusaka S,Lenz HJ

Published figure using CD45 monoclonal antibody (Product # 48-0459-42) in Immunocytochemistry

Tue Sep 28 00:00:00 EDT 2021

Targeting USP47 overcomes tyrosine kinase inhibitor resistance and eradicates leukemia stem/progenitor cells in chronic myelogenous leukemia.

Nature communications

Lei H,Xu HZ,Shan HZ,Liu M,Lu Y,Fang ZX,Jin J,Jing B,Xiao XH,Gao SM,Gao FH,Xia L,Yang L,Liu LG,Wang WW,Liu CX,Tong Y,Wu YZ,Zheng JK,Chen GQ,Zhou L,Wu YL

Published figure using CD45 monoclonal antibody (Product # 48-0459-42) in Immunohistochemistry

Mon Jan 04 00:00:00 EST 2021

Tracking HIV Rebound following Latency Reversal Using Barcoded HIV.

Cell reports. Medicine

Marsden MD,Zhang TH,Du Y,Dimapasoc M,Soliman MSA,Wu X,Kim JT,Shimizu A,Schrier A,Wender PA,Sun R,Zack JA

48-0459 was used in Flow cytometry/Cell sorting to study the effects of a designed, synthetic protein kinase C modulating Latency-Reversing Agents on HIV rebound.

Tue Dec 22 00:00:00 EST 2020

DKK2 blockage-mediated immunotherapy enhances anti-angiogenic therapy of Kras mutated colorectal cancer.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Hu J,Wang Z,Chen Z,Li A,Sun J,Zheng M,Wu J,Shen T,Qiao J,Li L,Li B,Wu D,Xiao Q

48-0459-42 was used in Flow Cytometry to demonstrate that if in human colorectal cancer tumor samples expressing high levels of DKK2, DKK2 blockade cause stronger activation of tumor infiltrating CD8+ T cells in ex vivo culture.

Wed Jul 01 00:00:00 EDT 2020

Side-by-side comparison of BH3-mimetics identifies MCL-1 as a key therapeutic target in AML.

Cell death & disease

Ewald L,Dittmann J,Vogler M,Fulda S

Published figure using CD45 monoclonal antibody (Product # 48-0459-42) in Flow Cytometry

Wed Dec 04 00:00:00 EST 2019

Mechanisms of virus dissemination in bone marrow of HIV-1-infected humanized BLT mice.

eLife

Ladinsky MS,Khamaikawin W,Jung Y,Lin S,Lam J,An DS,Bjorkman PJ,Kieffer C

Published figure using CD45 monoclonal antibody (Product # 48-0459-42) in Flow Cytometry

Mon Oct 28 00:00:00 EDT 2019

Functional Heterogeneity of Mouse Prostate Stromal Cells Revealed by Single-Cell RNA-Seq.

iScience

Kwon OJ,Zhang Y,Li Y,Wei X,Zhang L,Chen R,Creighton CJ,Xin L

48-0459 was used in Flow cytometry/Cell sorting to characterise mouse Prostate stromal cells heterogeneity by single-cell RNA-seq.

Fri Mar 29 00:00:00 EDT 2019

Human umbilical cord-derived mesenchymal stem cells ameliorate the enteropathy of food allergies in mice.

Experimental and therapeutic medicine

Yan N,Xu J,Zhao C,Wu Y,Gao F,Li C,Zhou W,Xiao T,Zhou X,Shao Q,Xia S

Published figure using CD45 monoclonal antibody (Product # 48-0459-42) in Flow Cytometry

Sat Dec 01 00:00:00 EST 2018

Single nucleotide variant profiles of viable single circulating tumour cells reveal CTC behaviours in breast cancer.

Oncology reports

Wang Y,Guo L,Feng L,Zhang W,Xiao T,Di X,Chen G,Zhang K

Published figure using CD45 monoclonal antibody (Product # 48-0459-42) in Flow Cytometry

Tue May 01 00:00:00 EDT 2018

DKK2 imparts tumor immunity evasion through β-catenin-independent suppression of cytotoxic immune-cell activation.

Nature medicine

Xiao Q,Wu J,Wang WJ,Chen S,Zheng Y,Yu X,Meeth K,Sahraei M,Bothwell ALM,Chen L,Bosenberg M,Chen J,Sexl V,Sun L,Li L,Tang W,Wu D

48-0459 was used in Flow cytometry/Cell sorting to identify a novel tumour immune-suppressive mechanism and immunotherapeutic targets particularly relevant for colorectal cancers and a subset of melanomas.

Thu Mar 01 00:00:00 EST 2018

Evaluation of a simple method for storage of blood samples that enables isolation of circulating tumor cells 96 h after sample collection.

Journal of biological research (Thessalonike, Greece)

Apostolou P,Ntanovasilis DA,Papasotiriou I

Published figure using CD45 monoclonal antibody (Product # 48-0459-42) in Flow Cytometry

Fri Dec 01 00:00:00 EST 2017

Evaluation of a simple method for storage of blood samples that enables isolation of circulating tumor cells 96 h after sample collection.

Journal of biological research (Thessalonike, Greece)

Apostolou P,Ntanovasilis DA,Papasotiriou I

Published figure using CD45 monoclonal antibody (Product # 48-0459-42) in Flow Cytometry

Fri Dec 01 00:00:00 EST 2017

Targeted Disruption of TCF12 Reveals HEB as Essential in Human Mesodermal Specification and Hematopoiesis.

Stem cell reports

Li Y,Brauer PM,Singh J,Xhiku S,Yoganathan K,Zúñiga-Pflücker JC,Anderson MK

Published figure using CD45 monoclonal antibody (Product # 48-0459-42) in Flow Cytometry

Tue Sep 12 00:00:00 EDT 2017

Preferential accumulation of regulatory T cells with highly immunosuppressive characteristics in breast tumor microenvironment.

Oncotarget

Syed Khaja AS,Toor SM,El Salhat H,Faour I,Ul Haq N,Ali BR,Elkord E

Published figure using CD45 monoclonal antibody (Product # 48-0459-42) in Flow Cytometry

Tue May 16 00:00:00 EDT 2017

Circulating tumor cell detection in hepatocellular carcinoma based on karyoplasmic ratios using imaging flow cytometry.

Scientific reports

Liu Z,Guo W,Zhang D,Pang Y,Shi J,Wan S,Cheng K,Wang J,Cheng S

Published figure using CD45 monoclonal antibody (Product # 48-0459-42) in Immunofluorescence

Fri Dec 23 00:00:00 EST 2016

Circulating tumor cell detection in hepatocellular carcinoma based on karyoplasmic ratios using imaging flow cytometry.

Scientific reports

Liu Z,Guo W,Zhang D,Pang Y,Shi J,Wan S,Cheng K,Wang J,Cheng S

Published figure using CD45 monoclonal antibody (Product # 48-0459-42) in Immunofluorescence

Fri Dec 23 00:00:00 EST 2016

Targeting methyltransferase PRMT5 eliminates leukemia stem cells in chronic myelogenous leukemia.

The Journal of clinical investigation

Jin Y,Zhou J,Xu F,Jin B,Cui L,Wang Y,Du X,Li J,Li P,Ren R,Pan J

Published figure using CD45 monoclonal antibody (Product # 48-0459-42) in Flow Cytometry

Mon Oct 03 00:00:00 EDT 2016

Kinase-independent function of RIP1, critical for mature T-cell survival and proliferation.

Cell death & disease

Dowling JP,Cai Y,Bertin J,Gough PJ,Zhang J

Published figure using CD45 monoclonal antibody (Product # 48-0459-42) in Flow Cytometry

Thu Sep 29 00:00:00 EDT 2016

Platelets confound the measurement of extracellular miRNA in archived plasma.

Scientific reports

Mitchell AJ,Gray WD,Hayek SS,Ko YA,Thomas S,Rooney K,Awad M,Roback JD,Quyyumi A,Searles CD

Published figure using CD45 monoclonal antibody (Product # 48-0459-42) in Flow Cytometry

Tue Sep 13 00:00:00 EDT 2016

Antithymocyte Globulin at Clinically Relevant Concentrations Kills Leukemic Blasts.

Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation

Dabas R,Lee R,Servito MT,Dharmani-Khan P,Modi M,van Slyke T,Luider J,Durand C,Larratt L,Brandwein J,Morris D,Daly A,Khan FM,Storek J

Sun May 01 00:00:00 EDT 2016

A 3D culture system enhances the ability of human bone marrow stromal cells to support the growth of limbal stem/progenitor cells.

Stem cell research

González S,Mei H,Nakatsu MN,Baclagon ER,Deng SX

Published figure using CD45 monoclonal antibody (Product # 48-0459-42) in Immunocytochemistry

Tue Mar 01 00:00:00 EST 2016

UTX inhibition as selective epigenetic therapy against TAL1-driven T-cell acute lymphoblastic leukemia.

Genes & development

Benyoucef A,Palii CG,Wang C,Porter CJ,Chu A,Dai F,Tremblay V,Rakopoulos P,Singh K,Huang S,Pflumio F,Hébert J,Couture JF,Perkins TJ,Ge K,Dilworth FJ,Brand M

Published figure using CD45 monoclonal antibody (Product # 48-0459-42) in Flow Cytometry

Tue Mar 01 00:00:00 EST 2016

Human esophageal myofibroblasts secrete proinflammatory cytokines in response to acid and Toll-like receptor 4 ligands.

American journal of physiology. Gastrointestinal and liver physiology

Gargus M,Niu C,Vallone JG,Binkley J,Rubin DC,Shaker A

Published figure using CD45 monoclonal antibody (Product # 48-0459-42) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2015

Retinoic acid regulates hematopoietic development from human pluripotent stem cells.

Stem cell reports

Rönn RE,Guibentif C,Moraghebi R,Chaves P,Saxena S,Garcia B,Woods NB

Published figure using CD45 monoclonal antibody (Product # 48-0459-42) in Flow Cytometry

Tue Feb 10 00:00:00 EST 2015

A Small Molecule β2 Integrin Agonist Improves Chronic Kidney Allograft Survival by Reducing Leukocyte Recruitment and Accompanying Vasculopathy.

Frontiers in medicine

Khan SQ,Guo L,Cimbaluk DJ,Elshabrawy H,Faridi MH,Jolly M,George JF,Agarwal A,Gupta V

Published figure using CD45 monoclonal antibody (Product # 48-0459-42) in Immunofluorescence

Fri Jan 16 00:00:00 EST 2015

Hypoxia-cultured human adipose-derived mesenchymal stem cells are non-oncogenic and have enhanced viability, motility, and tropism to brain cancer.

Cell death & disease

Feng Y,Zhu M,Dangelmajer S,Lee YM,Wijesekera O,Castellanos CX,Denduluri A,Chaichana KL,Li Q,Zhang H,Levchenko A,Guerrero-Cazares H,Quiñones-Hinojosa A

Published figure using CD45 monoclonal antibody (Product # 48-0459-42) in Flow Cytometry

Thu Dec 11 00:00:00 EST 2014

Caveolin-1 regulates chemokine receptor 5-mediated contribution of bone marrow-derived cells to dermal fibrosis.

Frontiers in pharmacology

Lee R,Perry B,Heywood J,Reese C,Bonner M,Hatfield CM,Silver RM,Visconti RP,Hoffman S,Tourkina E

Published figure using CD45 monoclonal antibody (Product # 48-0459-42) in Immunofluorescence

Thu Jun 26 00:00:00 EDT 2014

Caveolin-1 regulates chemokine receptor 5-mediated contribution of bone marrow-derived cells to dermal fibrosis.

Frontiers in pharmacology

Lee R,Perry B,Heywood J,Reese C,Bonner M,Hatfield CM,Silver RM,Visconti RP,Hoffman S,Tourkina E

Published figure using CD45 monoclonal antibody (Product # 48-0459-42) in Immunofluorescence

Thu Jun 26 00:00:00 EDT 2014

Microvesicles derived from human umbilical cord Wharton's jelly mesenchymal stem cells attenuate bladder tumor cell growth in vitro and in vivo.

PloS one

Wu S,Ju GQ,Du T,Zhu YJ,Liu GH

Published figure using CD45 monoclonal antibody (Product # 48-0459-42) in Flow Cytometry

Tue Oct 22 00:00:00 EDT 2013

Plasmacytoid dendritic cells are scarcely represented in the human gut mucosa and are not recruited to the celiac lesion.

Mucosal immunology

Ráki M,Beitnes AC,Lundin KE,Jahnsen J,Jahnsen FL,Sollid LM

48-0459 was used in Flow cytometry/Cell sorting to suggest that IFN-α is present in the celiac mucosa and IFN-λ is important in intestinal antiviral defense.

Sun Sep 01 00:00:00 EDT 2013

Engineering HIV-1-resistant T-cells from short-hairpin RNA-expressing hematopoietic stem/progenitor cells in humanized BLT mice.

PloS one

Ringpis GE,Shimizu S,Arokium H,Camba-Colón J,Carroll MV,Cortado R,Xie Y,Kim PY,Sahakyan A,Lowe EL,Narukawa M,Kandarian FN,Burke BP,Symonds GP,An DS,Chen IS,Kamata M

48-0459 was used in Flow cytometry/Cell sorting to investigate combination approaches against HIV, showing that short-hairpin RNA-expressing hematopoietic stem/progenitor cells can produce HIV-1-resistant T cells.

Mon Jun 24 00:00:00 EDT 2013

Retention of CD4+ CD25+ FoxP3+ regulatory T cells in the liver after therapy-induced hepatitis C virus eradication in humans.

Journal of virology

Claassen MA,de Knegt RJ,Janssen HL,Boonstra A

48-0459 was used in Flow cytometry/Cell sorting to provide insight into the dynamics of the immune response to hepatitis C virus and the effect of therapy on intrahepatic immunity.

Wed Jun 01 00:00:00 EDT 2011

Overexpression of sICAM-1 in the alveolar epithelial space results in an exaggerated inflammatory response and early death in Gram negative pneumonia.

Respiratory research

Mendez MP,Monroy YK,Du M,Preston AM,Tolle L,Lin Y,VanDussen KL,Samuelson LC,Standiford TJ,Curtis JL,Beck JM,Christensen PJ,Paine R

48-0459 was used in Flow cytometry/Cell sorting to conclude that alveolar sICAM-1 modulates pulmonary inflammation.

Wed Jan 19 00:00:00 EST 2011

Yield and characterization of subcutaneous human adipose-derived stem cells by flow cytometric and adipogenic mRNA analyzes.

Cytotherapy

Yu G,Wu X,Dietrich MA,Polk P,Scott LK,Ptitsyn AA,Gimble JM

Published figure using CD45 monoclonal antibody (Product # 48-0459-42) in Flow Cytometry

Thu Jul 01 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: CD45; CD45 antigen; L-CA; Leukocyte common antigen; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen; T220 and B220

Gene Aliases: B220; CD45; CD45R; GP180; L-CA; LCA; LY5; PTPRC; T200

UniProt ID: (Human) P08575

Entrez Gene ID: (Human) 5788

Molecular Function: protein phosphatase protein modifying enzyme