|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Storage buffer||PBS with 4mg/ml BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Commonly used, FITC conjugates provide relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility.
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells.
Analyte Specific Reagent
Dynamic change in natural killer cell type in the human ocular mucosa in situ as means of immune evasion by adenovirus infection.
MHCD4501-4 was used in flow cytometry to investigate the dynamics and characteristics of natural killer cell types in the human ocular mucosal surface in situ during infection with group D human adenoviruses.
|Yawata N,Selva KJ,Liu YC,Tan KP,Lee AW,Siak J,Lan W,Vania M,Arundhati A,Tong L,Li J,Mehta JS,Yawata M||Mucosal immunology (9:159)||2016|
Dielectrophoretic capture and genetic analysis of single neuroblastoma tumor cells.
MHCD4501-4 was used in flow cytometry to describe a novel, non-invasive, and adaptable method for the capture and genetic analysis of single tumor cells from cancer patients.
|Carpenter EL,Rader J,Ruden J,Rappaport EF,Hunter KN,Hallberg PL,Krytska K,O'Dwyer PJ,Mosse YP||Frontiers in oncology (4:null)||2014|
Evaluation of leukocyte stabilisation in TransFix-treated blood samples by flow cytometry and transmission electron microscopy.
MHCD4501-4 was used in flow cytometry to assess the effects of a TransFix-based stabilization technique on leukocyte scatter characteristics, immunophenotyping, membrane permeability, absolute cell counting and morphology.
|Canonico B,Zamai L,Burattini S,Granger V,Mannello F,Gobbi P,Felici C,Falcieri E,Reilly JT,Barnett D,Papa S||Journal of immunological methods (295:67)||2004|
Cell-derived microparticles circulate in healthy humans and support low grade thrombin generation.
MHCD4501-4 was used in flow cytometry to examine the numbers, cellular origin and thrombin-generating properties of microparticles in healthy individuals.
|Berckmans RJ,Nieuwland R,Böing AN,Romijn FP,Hack CE,Sturk A||Thrombosis and haemostasis (85:639)||2001|