Invitrogen

CD45 Monoclonal Antibody (OX1), Super Bright™ 436, eBioscience™

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CD45 Antibody in Flow Cytometry (Flow) — Rat splenocytes were stained with 1.0 µg of Mouse IgG1 kappa Isotype Control, Super Bright 436 (Product # 62-4714-82) (blue histogram) or 1.0 µg of CD45 Monoclonal Antibody, Super Bright 436 (purple histogram). Cells in the lymphocyte gate were used for analysis.

CD45 Antibody in Immunocytochemistry (ICC/IF) — FIGURE 1 Gene and protein expression pattern of the immortalized cell lines. (A) Schematic representation of the immortalization process. Right: Genes incorporated in 10-4A cells for immortalization (B) Semi-quantitative RT-PCR of ATII cell ( Sftpc , Abca3 ), ATI cell ( Hopx , Cav1 ), and mesenchymal cell ( Vim ) marker genes. N = 5 (C) Immunofluorescent staining of 10-4A cells for expression of epithelial cell (EpCAM), alveolar type II cell (ABCa3), alveolar type I cell (aquaporin 5, caveolin 1), leukocyte (CD45), and mesenchymal cell (vimentin) marker expression. 10-4A cells predominantly express mesenchymal marker vimentin and caveolin-1, that is also expressed in lung fibroblasts. Scale bar = 50 mum.

Product Details

62-0461-82

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	1.0 µg/test	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Rat
Published species	Not Applicable
Host/Isotype	Mouse / IgG1, kappa
Recommended Isotype Control	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Super Bright™ 436, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	OX1
Conjugate	Super Bright™ 436 APC APC-eFluor 780 eFluor 450 FITC PE PerCP-eFluor 710
Excitation/Emission Max	413/431 nm View spectra
Form	Liquid
Concentration	0.2 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2, with BSA
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_2784806

Product Specific Information

Description: This OX1 monoclonal antibody recognizes a non-polymorphic determinant of rat CD45, which is also known as the leukocyte common antigen. CD45 is expressed on all hematopoietic cells except erythrocytes and platelets. This type I transmembrane protein tyrosine phosphatase exists as a variety of different isoforms due to alternative splicing.

Applications Reported: This OX1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This OX1 antibody has been tested by flow cytometric analysis of rat splenocytes. This may be used at less than or equal to 1.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

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Product References

A Novel Fibroblast Reporter Cell Line for in vitro Studies of Pulmonary Fibrosis.

Frontiers in physiology

Nemeth J,Schundner A,Quast K,Winkelmann VE,Frick M

Published figure using CD45 monoclonal antibody (Product # 62-0461-82) in Immunocytochemistry

Thu Nov 12 00:00:00 EST 2020

Bifidobacteria shape host neural circuits during postnatal development by promoting synapse formation and microglial function.

Scientific reports

Luck B,Engevik MA,Ganesh BP,Lackey EP,Lin T,Balderas M,Major A,Runge J,Luna RA,Sillitoe RV,Versalovic J

Published figure using CD45 monoclonal antibody (Product # 62-0461-82) in Flow Cytometry

Fri May 08 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: CD45; L-CA; leucocyte common antigen; Leukocyte common antigen; leukocyte common antigen A; leukocyte common antigen B; Protein tyrosine phosphatase, receptor-type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T220 and B220

Gene Aliases: CD45; L-CA; Lca; Ptprc; RT7; T200

UniProt ID: (Rat) P04157

Entrez Gene ID: (Rat) 24699

Molecular Function: extracellular matrix protein protein phosphatase protein modifying enzyme

Function(s)

Process(es)

protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding heparin binding protein kinase regulator activity protein kinase binding heparan sulfate proteoglycan binding

CD45 Monoclonal Antibody (OX1), Super Bright™ 436, eBioscience™

Product Details

62-0461-82

Flow Cytometry (Flow)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

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