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Surface staining of mouse splenocytes with Rat IgG2b kappa Isotype Control FITC (top, open histogram) or Rat IgG2b kappa Isotype Control PE (bottom, open histogram), and Anti- Mouse CD45 FITC (top, filled histogram) or 0.015 ug of Anti-Mouse CD45 PE (bottom, filled histogram, A16325). Total viable cells were used for analysis.
|Tested species reactivity||Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rat / IgG2b, kappa|
|Immunogen||The CD45 antigen|
|Storage buffer||proprietary buffer|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Immunogenicity of undifferentiated and differentiated allogeneic mouse mesenchymal stem cells.
A16325 was used in flow cytometry to study the immunogenicity and immunomodulatory properties of bone marrow-derived mesenchymal stem cells using an allogeneic mouse model.
|Mukonoweshuro B,Brown CJ,Fisher J,Ingham E||Journal of tissue engineering (5:null)||2014|
B220; c polypeptide; CD45 antigen; CD45R; GP180; LCA; leukocyte common antigen; LY5; lymphocyte antigen 5; lymphocyte common antigen; protein tyrosine phosphatase; receptor type; receptor-type tyrosine-protein phosphatase C; T200 glycoprotein; T200 leukocyte common antigen
B220; Cd45; CD45R; L-CA; loc; Ly-5; Lyt-4; T200