|Tested species reactivity||Mouse|
|Published species reactivity||Mouse, Not Applicable|
|Host / Isotype||Rat / IgG2b, kappa|
|Immunogen||The CD45 antigen|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Flow Cytometry (Flow)||See 4 publications below|
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
HMGB1 enhances immune suppression by facilitating the differentiation and suppressive activity of myeloid-derived suppressor cells.
MCD4517 was used in flow cytometry to study the mechanism for the effect of HMGB1 on myeloid-derived suppressor cells
|Parker KH,Sinha P,Horn LA,Clements VK,Yang H,Li J,Tracey KJ,Ostrand-Rosenberg S||Cancer research (74:5723)||2014|
Interleukin-17-producing innate lymphoid cells and the NLRP3 inflammasome facilitate obesity-associated airway hyperreactivity.
MCD4517 was used in flow cytometry to elucidate the immunological pathways that lead to obesity-associated asthma.
|Kim HY,Lee HJ,Chang YJ,Pichavant M,Shore SA,Fitzgerald KA,Iwakura Y,Israel E,Bolger K,Faul J,DeKruyff RH,Umetsu DT||Nature medicine (20:54)||2014|
Innate lymphoid cells mediate influenza-induced airway hyper-reactivity independently of adaptive immunity.
MCD4517 was used in flow cytometry to examine how influenza infection induced airway hyper-reactivity.
|Chang YJ,Kim HY,Albacker LA,Baumgarth N,McKenzie AN,Smith DE,Dekruyff RH,Umetsu DT||Nature immunology (12:631)||2011|
|Not Applicable||Not Cited||
T-cell regulation of neutrophil infiltrate at the early stages of a murine colitis model.
MCD4517 was used in flow cytometry to examine the effect of tacrolimus in a murine colitis model
|van Lierop PP,de Haar C,Lindenbergh-Kortleve DJ,Simons-Oosterhuis Y,van Rijt LS,Lambrecht BN,Escher JC,Samsom JN,Nieuwenhuis EE||Inflammatory bowel diseases (16:442)||2010|