|Tested species reactivity||Mouse|
|Published species reactivity||Mouse, Not Applicable|
|Host / Isotype||Rat / IgG2b, kappa|
|Immunogen||The CD45 antigen|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Eradication of metastatic mouse cancers resistant to immune checkpoint blockade by suppression of myeloid-derived cells.
MCD4530 was used in flow cytometry to identify a relationship between resistant cancers and myeloid-derived suppressor cells
|Kim K,Skora AD,Li Z,Liu Q,Tam AJ,Blosser RL,Diaz LA,Papadopoulos N,Kinzler KW,Vogelstein B,Zhou S||Proceedings of the National Academy of Sciences of the United States of America (111:11774)||2014|
CD4+ T lymphocyte ablation prevents pancreatic carcinogenesis in mice.
MCD4530 was used in flow cytometry to determine the role of CD4+ T cells during mutant Kras-driven pancreatic carcinogenesis
|Zhang Y,Yan W,Mathew E,Bednar F,Wan S,Collins MA,Evans RA,Welling TH,Vonderheide RH,di Magliano MP||Cancer immunology research (2:423)||2014|
Interleukin-6 is required for pancreatic cancer progression by promoting MAPK signaling activation and oxidative stress resistance.
MCD4530 was used in flow cytometry to demonstrate that IL-6 is required for the maintenance and progression of pancreatic cancer precursor lesions.
|Zhang Y,Yan W,Collins MA,Bednar F,Rakshit S,Zetter BR,Stanger BZ,Chung I,Rhim AD,di Magliano MP||Cancer research (73:6359)||2013|
Nanoparticle clearance is governed by Th1/Th2 immunity and strain background.
MCD4530 was used in flow cytometry to investigate nanoparticle clearance.
|Jones SW,Roberts RA,Robbins GR,Perry JL,Kai MP,Chen K,Bo T,Napier ME,Ting JP,Desimone JM,Bear JE||The Journal of clinical investigation (123:3061)||2013|
|Not Applicable||Not Cited||
Mesenchymal stem cells are mobilized from the bone marrow during inflammation.
MCD4530 was used in flow cytometry to study the impact of mesenchymal stem cells to experimental allergic encephalomyelitis
|Koning JJ,Kooij G,de Vries HE,Nolte MA,Mebius RE||Frontiers in immunology (4:null)||2013|
Impact of isolated germline JAK2V617I mutation on human hematopoiesis.
MCD4530 was used in flow cytometry to describe a germline JAK2V617I mutation in a family with hereditary thrombocytosis and characterize the hematopoietic and signaling impact of this mutation.
|Mead AJ,Chowdhury O,Pecquet C,Dusa A,Woll P,Atkinson D,Burns A,Score J,Rugless M,Clifford R,Moule S,Bienz N,Vyas P,Cross N,Gale RE,Henderson S,Constantinescu SN,Schuh A,Jacobsen SE||Blood (121:4156)||2013|
TIM-3 regulates innate immune cells to induce fetomaternal tolerance.
MCD4530 was used in flow cytometry to determine the role of TIM-3-expressing innate immune cells in the regulation of tolerance at the fetomaternal interface.
|Chabtini L,Mfarrej B,Mounayar M,Zhu B,Batal I,Dakle PJ,Smith BD,Boenisch O,Najafian N,Akiba H,Yagita H,Guleria I||Journal of immunology (Baltimore, Md. : 1950) (190:88)||2013|
MyD88-dependent recruitment of monocytes and dendritic cells required for protection from pulmonary Burkholderia mallei infection.
MCD4530 was used in flow cytometry to identify key Toll-like receptor signaling pathways, effector cells, and cytokines required for protection from respiratory B. mallei infection.
|Goodyear A,Troyer R,Bielefeldt-Ohmann H,Dow S||Infection and immunity (80:110)||2012|