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Western blot analysis of CD45 was performed using membrane enriched extracts (30 ug lysate) of KARPAS 299 (Lane 1) and THP-1 (Lane 2). The blots were probed with Anti-CD45 Mouse Monoclonal Antibody (Product # MA5-12795, 2 µg/ml) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.4 µg/ml, 1:2500 dilution). A 220 kDa band corresponding to CD45 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with overnight wet transfer system. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Non-T, non-B ALL cell line (REH)|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-12795 targets CD45 in FACS and IF applications and shows reactivity with Human samples.
The MA5-12795 immunogen is non-T, non-B ALL cell line (REH).
CD45 leucocyte common antigen (LCA) belongs to the family of at least four isoforms of membrane glycoproteins (220, 205, 190, 180kDa) expressed on hematopoietic cell lines but absent on non-hematopoietic cell lines, normal and malignant non-hematopoietic tissues. The intracellular portion of these molecules have protein phosphatase activity and are involved in regulation of transmembrane signals.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
B220; c polypeptide; CD45; CD45 antigen; CD45R; GP180; L-CA; LCA; LY5; protein tyrosine phosphatase; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; receptor type; receptor-type tyrosine-protein phosphatase C; T200 glycoprotein; T200 leukocyte common antigen
B220; CD45; CD45R; GP180; L-CA; LCA; LY5; PTPRC; T200