Histogram overlay plot of gated human lymphocytes, the black line represents cells labeled with CD45 Mouse Anti-Human mAb (clone HI30), Qdot® 655 Conjugate and the gray line represents unstained cells. Samples were acquired and analyzed using 405 nm excitation and a 660/20 band pass emission filter using the Attune® Acoustic Cytometer Blue/Violet option (Life Technologies, Carlsbad CA).
|Tested species reactivity||Human|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Storage buffer||0.05M borate, pH 8.3, with 1M betaine|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Dynamic change in natural killer cell type in the human ocular mucosa in situ as means of immune evasion by adenovirus infection.
Q22154 was used in flow cytometry to investigate the dynamics and characteristics of natural killer cell types in the human ocular mucosal surface in situ during infection with group D human adenoviruses.
|Yawata N,Selva KJ,Liu YC,Tan KP,Lee AW,Siak J,Lan W,Vania M,Arundhati A,Tong L,Li J,Mehta JS,Yawata M||Mucosal immunology (9:159)||2016|
|Not Applicable||Not Cited||
Transcriptional regulation of Munc13-4 expression in cytotoxic lymphocytes is disrupted by an intronic mutation associated with a primary immunodeficiency.
Q22154 was used in flow cytometry to find Munc13-4 is highly expressed in differentiated human natural killer cells and effector CD8(+) T lymphocytes
|Cichocki F,Schlums H,Li H,Stache V,Holmes T,Lenvik TR,Chiang SC,Miller JS,Meeths M,Anderson SK,Bryceson YT||The Journal of experimental medicine (211:1079)||2014|
Telomere attrition occurs during ex vivo expansion of human dental pulp stem cells.
Q22154 was used in flow cytometry to characterize stem cells isolated and expanded from the human dental pulp
|Mokry J,Soukup T,Micuda S,Karbanova J,Visek B,Brcakova E,Suchanek J,Bouchal J,Vokurkova D,Ivancakova R||Journal of biomedicine and biotechnology (2010:null)||2010|