|Immunohistochemistry (Paraffin) (IHC (P))||Ready-to-use|
|Tested Species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Stimulated human leucocytes.|
|Storage buffer||TBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is tonsil tissue.
CD45 leucocytescommon antigen (LCA) belongs to a family of at least four isoforms of membrane glycoproteins (220, 205, 190, 180kDa) expressed on hematopoietic cell lines but absent on non-hematopoietic cell lines, normal and malignant non hematopoietic tissues. The intracellular portion of these molecules has protein phosphatase activity and is inovled in regulation of transmemebrane signals.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: B220; c polypeptide; CD45; CD45 antigen; CD45R; GP180; L-CA; LCA; Leukocyte common antigen; LY5; protein tyrosine phosphatase; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; receptor type; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen
Gene Aliases: B220; CD45; CD45R; GP180; L-CA; LCA; LY5; PTPRC; T200
UniProt ID: (Human) P08575
Entrez Gene ID: (Human) 5788
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