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|Tested species reactivity||Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rat / IgG2b|
|Immunogen||Murine spleen cells.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1/100 - 1/200|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 2 publications below|
For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Attenuation of traumatic brain injury-induced cognitive impairment in mice by targeting increased cytokine levels with a small molecule experimental therapeutic.
MA1-81247 was used in immunohistochemistry to determine therapeutic window for MW151 efficacy to treat traumatic brain injury
|Bachstetter AD,Webster SJ,Goulding DS,Morton JE,Watterson DM,Van Eldik LJ||Journal of neuroinflammation (12:null)||2015|
The p38α MAPK regulates microglial responsiveness to diffuse traumatic brain injury.
MA1-81247 was used in immunohistochemistry to study the response of microglia to diffuse traumatic brain injury and the modulatory role of p38alpha MAPK in a murine model
|Bachstetter AD,Rowe RK,Kaneko M,Goulding D,Lifshitz J,Van Eldik LJ||The Journal of neuroscience : the official journal of the Society for Neuroscience (33:6143)||2013|
B220; c polypeptide; CD45; CD45 antigen; CD45R; GP180; L-CA; LCA; leukocyte common antigen; LY5; lymphocyte antigen 5; lymphocyte common antigen; protein tyrosine phosphatase; receptor type; receptor-type tyrosine-protein phosphatase C; T200 glycoprotein; T200 leukocyte common antigen
B220; Cd45; CD45R; L-CA; loc; Ly-5; Lyt-4; T200