|Tested species reactivity||Mouse|
|Published species reactivity||Mouse, Not Applicable|
|Host / Isotype||Rat / IgG2a, kappa|
|Immunogen||exon A-specific epitope on CD45|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
CD45/CD11b positive subsets of adult lung anchorage-independent cells harness epithelial stem cells in culture.
RM2606 was used in flow cytometry to examine the relationship between stem/progenitor cells that proliferate or differentiate and somatic cells of the lung.
|Peter Y,Sen N,Levantini E,Keller S,Ingenito EP,Ciner A,Sackstein R,Shapiro SD||Journal of tissue engineering and regenerative medicine (7:572)||2013|
|Not Applicable||Not Cited||
Identification of a myeloid committed progenitor as the cancer-initiating cell in acute promyelocytic leukemia.
RM2606 was used in flow cytometry to study committed transformed progenitors in a murine model of acute promyelocytic leukemia
|Guibal FC,Alberich-Jorda M,Hirai H,Ebralidze A,Levantini E,Di Ruscio A,Zhang P,Santana-Lemos BA,Neuberg D,Wagers AJ,Rego EM,Tenen DG||Blood (114:5415)||2009|
|Not Applicable||Not Cited||
Inducible chronic phase of myeloid leukemia with expansion of hematopoietic stem cells in a transgenic model of BCR-ABL leukemogenesis.
RM2606 was used in flow cytometry to develop murine models of leukemogenesis
|Koschmieder S,Göttgens B,Zhang P,Iwasaki-Arai J,Akashi K,Kutok JL,Dayaram T,Geary K,Green AR,Tenen DG,Huettner CS||Blood (105:324)||2005|
Reduced development of CD4-8-B220+ T cells but normal autoantibody production in lpr/lpr mice lacking major histocompatibility complex class I molecules.
RM2606 was used in flow cytometry to examine the origin of double negative T cells using Ipr mice.
|Ohteki T,Iwamoto M,Izui S,MacDonald HR||European journal of immunology (25:37)||1995|