Immunohistochemical staining of paraformaldehyde-fixed paraffin-embedded human spleen sections at 400x magnification with DAPI counterstain. Primary antibody used was a CD45R monoclonal antibody (clone YTH80.103) at a 1:500 dilution, followed by secondary antibody was a 488 fluorescent conjugated Goat anti-rat at a 1:500 dilution and Normal Goat Serum (blocking)
|Tested species reactivity||Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rat / IgG2b|
|Immunogen||Rat monoclonal antibody raised against human CD45R|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1.0 ug/10^6 cells|
|Immunohistochemistry (Paraffin) (IHC (P))||1:500|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Flow Cytometry (Flow)||See 1 publications below|
MA5-17692 targets CD45R in FACS and WB applications and shows reactivity with Human samples.
The MA5-17692 immunogen is rat monoclonal antibody raised against human CD45R.
CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
A novel mutation in PTPRC interferes with splicing and alters the structure of the human CD45 molecule.
MA5-17692 was used in flow cytometry to identify a mutation in PTPRCas a cause of variant CD45 expression in humans
|Jacobsen M,Hoffmann S,Cepok S,Stei S,Ziegler A,Sommer N,Hemmer B||Immunogenetics (54:158)||2002|