|Tested species reactivity||Human|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgG2b|
|Storage buffer||0.05M borate, pH 8.3, with 1M betaine|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Human neonatal naive CD4+ T cells have enhanced activation-dependent signaling regulated by the microRNA miR-181a.
Q10047 was used in flow cytometry to test if impaired human neonatal CD4(+) T cell immunity is due to reduced signaling by naive CD4(+) T cells following engagement of the αβ-TCR/CD3 complex and CD28.
|Palin AC,Ramachandran V,Acharya S,Lewis DB||Journal of immunology (Baltimore, Md. : 1950) (190:2682)||2013|
Unexpected heterogeneity of multifunctional T cells in response to superantigen stimulation in humans.
Q10047 was used in flow cytometry to examine the role of IL-17A-producing multifunctional T cells in the pathogenesis of toxic shock syndrome.
|McArthur MA,Sztein MB||Clinical immunology (Orlando, Fla.) (146:140)||2013|
Heterogeneity of multifunctional IL-17A producing S. Typhi-specific CD8+ T cells in volunteers following Ty21a typhoid immunization.
Q10047 was used in flow cytometry to explore the multifunctional IL-17A responses against S. Typhi antigens in T memory subsets.
|McArthur MA,Sztein MB||PloS one (7:null)||2012|
Phase 1 study of stereotactic body radiotherapy and interleukin-2--tumor and immunological responses.
Q10047 was used in flow cytometry to report results from a pilot study of stereotactic body radiation therapy followed by high-dose interleukin-2 that assessed safety and tumor response rates.
|Seung SK,Curti BD,Crittenden M,Walker E,Coffey T,Siebert JC,Miller W,Payne R,Glenn L,Bageac A,Urba WJ||Science translational medicine (4:null)||2012|
|Not Applicable||Not Cited||
Leukocyte composition of human breast cancer.
Q10047 was used in flow cytometry to examine tumor and nonadjacent normal breast tissue from women with breast cancer, who either had or had not received neoadjuvant chemotherapy before surgery
|Ruffell B,Au A,Rugo HS,Esserman LJ,Hwang ES,Coussens LM||Proceedings of the National Academy of Sciences of the United States of America (109:2796)||2012|