|Tested species reactivity||Human|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Human thymocytes and T lymphocytes.|
|Storage buffer||PBS with 0.2% BSA|
|Contains||15mM sodium azide|
|Storage Conditions||4° C, store in dark, DO NOT FREEZE!|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||20ul x 100ul whole blood or 10^6 cells|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
CD45RA is a high molecular weight isoform of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RA is expressed e.g. on naïve T cells and normal plasma cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
The JAK2 V617F mutation occurs in hematopoietic stem cells in polycythemia vera and predisposes toward erythroid differentiation.
MA1-19570 was used in flow cytometry to detect the JAK2 V617F mutation in hematopoietic stem cells and their progeny in patients with polycythemia vera
|Jamieson CH,Gotlib J,Durocher JA,Chao MP,Mariappan MR,Lay M,Jones C,Zehnder JL,Lilleberg SL,Weissman IL||Proceedings of the National Academy of Sciences of the United States of America (103:6224)||2006|
Prospective isolation of human clonogenic common myeloid progenitors.
MA1-19570 was used in flow cytometry to report how to isolate highly purified hematopoietic intermediates.
|Manz MG,Miyamoto T,Akashi K,Weissman IL||Proceedings of the National Academy of Sciences of the United States of America (99:11872)||2002|