|Tested species reactivity||Human|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgG2b|
|Storage buffer||PBS with 4mg/ml BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
T lymphocytes and fractalkine contribute to myocardial ischemia/reperfusion injury in patients.
MHCD45RA28 was used in flow cytometry to investigate the contribution of lymphocytes to primary percutaneous coronary intervention
|Boag SE,Das R,Shmeleva EV,Bagnall A,Egred M,Howard N,Bennaceur K,Zaman A,Keavney B,Spyridopoulos I||The Journal of clinical investigation (125:3063)||2015|
Chronic unpredictable mild stress impairs erythrocyte immune function and changes T-lymphocyte subsets in a rat model of stress-induced depression.
MHCD45RA28 was used in flow cytometry to examine the changes in erythrocytes and T-lymphocytes and to explore the mechanisms involved in stress-induced depression using a rat depression model.
|Guan SZ,Liu JW,Fang EF,Ng TB,Lian YL,Ge H||Environmental toxicology and pharmacology (37:414)||2014|
Expression of integrin ¿2 receptor in human cord blood CD34+CD38-CD90+ stem cells engrafting long-term in NOD/SCID-IL2R¿(c) null mice.
MHCD45RA28 was used in flow cytometry to identify integrin α2 as a novel stem cell marker.
|Wong WM,Sigvardsson M,Åstrand-Grundström I,Hogge D,Larsson J,Qian H,Ekblom M||Stem cells (Dayton, Ohio) (31:360)||2013|
Th17 central memory T cells are reduced by FTY720 in patients with multiple sclerosis.
MHCD45RA28 was used in flow cytometry to determine the effect of FTY720 treatment on Th17 cells
|Mehling M,Lindberg R,Raulf F,Kuhle J,Hess C,Kappos L,Brinkmann V||Neurology (75:403)||2010|
Functional regulatory T cells accumulate in aged hosts and promote chronic infectious disease reactivation.
MHCD45RA28 was used in flow cytometry to characterize Tregs from older subjects and mice.
|Lages CS,Suffia I,Velilla PA,Huang B,Warshaw G,Hildeman DA,Belkaid Y,Chougnet C||Journal of immunology (Baltimore, Md. : 1950) (181:1835)||2008|
Proteome-wide analysis of HIV-specific naive and memory CD4(+) T cells in unexposed blood donors.
MHCD45RA28 was used in immunocytochemistry to analyze HIV-specific naive and memory CD4(+) T cells through two methods.
|Campion SL,Brodie TM,Fischer W,Korber BT,Rossetti A,Goonetilleke N,McMichael AJ,Sallusto F||The Journal of experimental medicine (211:1273)||2014|
CD4 and CD8 T cell immune activation during chronic HIV infection: roles of homeostasis, HIV, type I IFN, and IL-7.
MHCD45RA28 was used in flow cytometry to investigate the regulation of the CD4 and CD8 T cell pools during HIV infection.
|Catalfamo M,Wilhelm C,Tcheung L,Proschan M,Friesen T,Park JH,Adelsberger J,Baseler M,Maldarelli F,Davey R,Roby G,Rehm C,Lane C||Journal of immunology (Baltimore, Md. : 1950) (186:2106)||2011|
|Not Applicable||Not Cited||
In vivo evaluation of human hematopoiesis through xenotransplantation of purified hematopoietic stem cells from umbilical cord blood.
MHCD45RA28 was used in flow cytometry to develop a xenotransplantation system using human hematopoietic stem cells and mice
|Park CY,Majeti R,Weissman IL||Nature protocols (3:1932)||2009|
|Not Applicable||Not Cited||
Comparative study of regulatory T cell function of human CD25CD4 T cells from thymocytes, cord blood, and adult peripheral blood.
MHCD45RA28 was used in flow cytometry to assess the regulatory function of human CD25+CD4+ T cells at various stages of maturity
|Fujimaki W,Takahashi N,Ohnuma K,Nagatsu M,Kurosawa H,Yoshida S,Dang NH,Uchiyama T,Morimoto C||Clinical and developmental immunology (2008:null)||2008|