|Tested species reactivity||Human|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgG2b|
|Storage buffer||0.05M borate, pH 8.3, with 1M betaine|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Optimization of the cytokine secretion assay for human IL-2 in single and combination assays.
Q10069 was used in flow cytometry to optimize detection of IL-2 from live cells
|Deng N,Mosmann TR||Cytometry. Part A : the journal of the International Society for Analytical Cytology (87:777)||2015|
|Not Applicable||Not Cited||
Cytokine diversity in the Th1-dominated human anti-influenza response caused by variable cytokine expression by Th1 cells, and a minor population of uncommitted IL-2+IFN¿- Thpp cells.
Q10069 was used in flow cytometry to investigate the anti-influenza responses of T cell subsets
|Deng N,Weaver JM,Mosmann TR||PloS one (9:null)||2014|
Ki-67 expression reveals strong, transient influenza specific CD4 T cell responses after adult vaccination.
Q10069 was used in flow cytometry to study influenza-specific CD4 T cells respond to vaccination using Ki67 as a marker.
|Li X,Miao H,Henn A,Topham DJ,Wu H,Zand MS,Mosmann TR||Vaccine (30:4581)||2012|
CD8+ T cell immunity to 2009 pandemic and seasonal H1N1 influenza viruses.
Q10069 was used in flow cytometry to examine human cross-reactive T cells against a pandemic virus.
|Scheible K,Zhang G,Baer J,Azadniv M,Lambert K,Pryhuber G,Treanor JJ,Topham DJ||Vaccine (29:2159)||2011|