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|Tested species reactivity||Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified rat spleen leucocyte common antigen.|
|Storage buffer||PBS, pH 7.2|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1/50 - 1/100|
|Immunohistochemistry (Paraffin, Frozen) (IHC (P, F))||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-81427 is directed against a high molecular weight band of the leucocyte common antigen (LCA). This clone only labels B-cells among thoracic duct lymphocytes, demonstrating low reactivity with bone marrow and no reactivity with thymocytes.
PLP fixation is recommended for optimal tissue staining results. For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
B220; CD45; CD45R; GP180; LCA; leucocyte common antigen; leukocyte common antigen A; leukocyte common antigen B; LY5; Protein tyrosine phosphatase, receptor-type, c polypeptide; receptor-type tyrosine-protein phosphatase C; T200 glycoprotein
CD45; L-CA; Lca; Ptprc; RT7; T200