|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Human peripheral blood lymphocytes maintained in T cell growth factor|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.5 ug/test|
|Immunohistochemistry (Paraffin) (IHC (P))||1-2 µg/ml|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 2 publications below|
MA5-12496 targets CD45RB in FACS, Western blot and IHC (P) applications and shows reactivity with Human and Mouse samples. This antibody is not recommended for BAF-3 cells in Western blot applications.
The MA5-12496 immunogen is human peripheral blood lymphocytes maintained in T cell growth factor.
CD45RB is expressed on mature B lymphocytes and the majority of lymphomas and leukemias of B-cell origin. This antigen is also expressed on the surface of cells of myeloid lineage and in the cytoplasm of tissue macrophages.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
PDGFR-ß (+) perivascular cells from infantile hemangioma display the features of mesenchymal stem cells and show stronger adipogenic potential in vitro and in vivo.
MA5-12496 was used in immunocytochemistry to isolate and characterize PDGFR-beta(+) perivascular cells from infantile hemangioma
|Yuan SM,Guo Y,Zhou XJ,Shen WM,Chen HN||International journal of clinical and experimental pathology (7:2861)||2014|
Mesenchymal stem cells in infantile hemangioma reside in the perivascular region.
MA5-12496 was used in immunocytochemistry to test if MSCs in hemangioma also reside in the perivascular region
|Yuan SM,Chen RL,Shen WM,Chen HN,Zhou XJ||Pediatric and developmental pathology : the official journal of the Society for Pediatric Pathology and the Paediatric Pathology Society (15:5)||2012|