|Flow Cytometry (Flow)||2 ug/ml|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
|Tested Species reactivity||Human, Non-human primate, Pig|
|Host / Isotype||Mouse / IgM|
|Immunogen||COS-7 (African green monkey) cells.|
|Storage buffer||TBS, pH 8|
|Contains||15mM sodium azide|
|Storage conditions||4° C, do not freeze|
This antibody is purified from ascites by CHT-chromatagraphy and precipitation methods.
CD47 (integrin-associated protein, IAP) is an ubiquitously expressed cell surface transmembrane glycoprotein interacting with several integrins and regulating their functions. Engagement of CD47 by soluble ligands or counter receptors modulates various signaling pathways, such as activation of heterotrimeric G proteins. Binding secreted thrombospondin-1, CD47 counteracts graft vascularization. CD47 acts also as a ligand for CD172a (signal regulatory protein alpha, SIRP alpha), an immune inhibitory receptor on macrophages; this interaction prevents phagocytosis of CD47-positive cells. Moreover, CD47-CD172a system affects cell migration, B cell adhesion and T cell activation. CD47 is also involved in modulation of chondrocyte responses to mechanical signals, and promotes neuronal development, being especially abundant in synapse-rich regions of brain and retina.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: antigen identified by monoclonal antibody 1D8; Antigenic surface determinant protein OA3; CD47; CD47 antigen; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); CD47 glycoprotein; IAP; integrin associated protein; Integrin-associated protein; integrin-associated signal transducer; Leukocyte surface antigen CD47; MER6; OA3; Protein MER6; Rh-related antigen
Gene Aliases: CD47; CD47/IAP; IAP; MER6; OA3
Molecular Function: G-protein modulator cell adhesion molecule cytokine receptor defense/immunity protein enzyme modulator guanyl-nucleotide exchange factor immunoglobulin receptor superfamily receptor
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