Description: The HMalpha2 monoclonal antibody reacts with mouse CD49b, the 150 kDa integrin alpha2 subunit. The complex of CD49b non-covalently associated with integrin beta1 (CD29), also known as VLA-2, is a receptor for collagen and laminin. This complex is expressed by some CD4^+ T cells, IEL, NK cells, platelets and epithelial cells.
Applications Reported: The HM alpha2 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue (IHC-F). It has also been reported for use in in vitro functional assays (Please use Functional Grade purified HMa2, cat. 16-0491, in functional assays.).
Applications Tested: The HM alpha2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
GTP-binding proteins (G-proteins) play a critical role in signal transduction by coupling receptors to effectors. The intracellular consequences of G-protein activation include second messenger generation, protein phosphorylation, ion channel activation, gene induction, cell growth, and differentiation. Gi belongs to the pertussis toxin sensitive G-proteins. Gi consists of three different subtypes of a-subunits (ai1, ai2 and ai3).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: BR; CD49 antigen-like family member B; CD49B; Collagen receptor; GPIa; Integrin alpha-2; ITGA2; Platelet membrane glycoprotein Ia; VLA-2; VLA-2 receptor, alpha 2 subunit; VLA-2 subunit alpha; VLAA2
Gene Aliases: CD49B; DX5; Itga2
UniProt ID: (Mouse) Q62469
Entrez Gene ID: (Mouse) 16398