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CD49b (Integrin alpha 2) Recombinant Rabbit Monoclonal Antibody (SN0752)

View all (61) CD49b (Integrin alpha 2) antibodies

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Protocols

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CD49b (Integrin alpha 2) Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemical analysis of CD49b (Integrin alpha 2) in MCF-7 cells using a CD49b (Integrin alpha 2) Monoclonal antibody (Product # MA5-32306) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

CD49b (Integrin alpha 2) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of CD49b (Integrin alpha 2) of paraffin-embedded Human breast carcinoma tissue using a CD49b-Integrin-alpha-2 Monoclonal antibody (Product #MA5-32306). Counter stained with hematoxylin.

CD49b (Integrin alpha 2) Antibody in Western Blot (WB) — Western blot was performed using Anti-CD49b (Integrin alpha 2) Recombinant Rabbit Monoclonal Antibody (SN0752) (Product # MA5-32306) and bands at 160, 120 kDa corresponding to CD49b were observed across the cell lines and tissue tested except U-937 which is reported to be negative. Membrane enriched extracts (30 µg lysate) of MCF7 (Lane 1), MDA-MB-231 (Lane 2), T-47D (Lane 3), A-431 (Lane 4), U-2 OS (Lane 5), HEK-293 (Lane 6), A549 (Lane 7), U-937 (Lane 8) and Mouse Spleen (Lane 9) were electrophoresed using Novex® NuPAGE® 4-12% % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

CD49b (Integrin alpha 2) Antibody in Flow Cytometry (Flow) — Flow Cytometric analysis of CD49b (Integrin alpha 2) in A431 cells using a CD49b (Integrin alpha 2) Monoclonal Antibody (Product # MA5-32306) at a dilution of 1:50, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Product Details

MA5-32306

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:5,000	-
Immunohistochemistry (IHC)	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:100-1:500	-
Flow Cytometry (Flow)	1:50-1:100	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Recombinant Monoclonal
Type	Antibody
Clone	SN0752
Immunogen	Synthetic peptide within Human Integrin alpha 2 aa 1,132-1,181 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains	0.05% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2809588

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

CD49b (Integrin alpha 2, Integrin alpha 2 chain, VLA-2 alpha chain, HM alpha 2) is a member of the integrin family. It is a glycoprotein with molecular weight of 150 kD, and it complexes with CD29 (Integrin beta 1) to form the heterodimeric integrin VLA-2 (integrin alpha 2 beta 1, or GPIa/IIa) complex. VLA-2 is an extracellular receptor for laminin, collagen, and fibronectin, and interaction with its ligands results in the activation of intracellular signaling pathways. It has reported roles in VEGF-induced angiogenesis in vivo, as well as adhesion and lymphocyte activation. CD49b is expressed by NK cells, NK-T cells, monocytes, platelets, and epithelial cells. It is also expressed on adaptive immune cells such as T and B cells, specifically on a subset of CD4+ T cells in the spleen, on intraepithelial and lamina propria lymphocytes in the intestine, as well as on a population of peripheral CD4+ type 1 T regulatory (Tr1) cells that co-express LAG-3.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: alpha 2 subunit of VLA-2 receptor; BDPLT9; CD49 antigen-like family member B; CD49b; Collagen receptor; GPIa; human platelet alloantigen system 5; Integrin alpha-2; integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor); platelet antigen Br; platelet glycoprotein GPIa; Platelet membrane glycoprotein Ia; very late activation protein 2 receptor, alpha-2 subunit; VLA-2 receptor, alpha 2 subunit; VLA-2 subunit alpha; VLA2 receptor

Gene Aliases: BR; CD49B; DX5; GPIa; HPA-5; ITGA2; VLA-2; VLAA2

UniProt ID: (Human) P17301, (Mouse) Q62469

Entrez Gene ID: (Human) 3673, (Mouse) 16398, (Rat) 170921

Molecular Function: integrin

Function(s)

Process(es)

virus receptor activity integrin binding protein binding collagen binding protein complex binding collagen receptor activity laminin binding metal ion binding protein heterodimerization activity collagen binding involved in cell-matrix adhesion cell adhesion molecule binding

CD49b (Integrin alpha 2) Recombinant Rabbit Monoclonal Antibody (SN0752)

Product Details

MA5-32306

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

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