|Tested species reactivity||Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||C3H.CE - Ly 1.2 : DS|
|Storage buffer||PBS with 4-5mg/ml BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.2 ug/10^6 cells|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 2 publications below|
MA5-17787 targets CD5/Ly-1 in FACS applications and shows reactivity with Mouse samples.
The MA5-17787 immunogen is c3H.CE - Ly 1.2 : DS.
The CD5, a transmembrane protein, is found on 95%of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. CD5 is expressed by many T cell leukemia, lymphomas, and activated T cells. Occasionally, CD5 antigen is also expressed on a subset of B cells. Mantle cell lymphomas (same as diffuse centrocytic lymphomas) are CD5+ while the follicle center cell lymphoma are CD5-.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Involvement of p38 mitogen-activated protein kinase in different stages of thymocyte development.
MA5-17787 was used in flow cytometry to suggest that p38 is involved in positive selection of T cells.
|Hsu SC,Wu CC,Han J,Lai MZ||Blood (101:970)||2003|
Increased p300 expression inhibits glucocorticoid receptor-T-cell receptor antagonism but does not affect thymocyte positive selection.
MA5-17787 was used in flow cytometry to test if T-cell-specific expression of p300 alters GR-TCR cross talk between thymocytes.
|Yu CT,Feng MH,Shih HM,Lai MZ||Molecular and cellular biology (22:4556)||2002|