Invitrogen

CD5 Monoclonal Antibody (53-7.3), PerCP-Cyanine5.5, eBioscience™

Product Image Gallery

CD5 Antibody in Flow Cytometry (Flow) — Staining of C57BL/6 splenocytes with Anti-Human/Mouse CD45R (B220) APC (Product # 17-0452-82) and 0.06 µg of Rat IgG2a K Isotype Control PerCP-Cyanine5-5 (Product # 45-4321-80) (left) or 0.06 µg of Anti-Mouse CD5 PerCP-Cyanine5-5 (right). Total viable cells were used for analysis.

Product Details

45-0051-82

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	0.125 µg/test	View 19 publications 19 publications

Product Specifications
Species Reactivity	Mouse
Published species	Human, Mouse
Host/Isotype	Rat / IgG2a, kappa
Recommended Isotype Control	Rat IgG2a kappa Isotype Control (eBR2a), PerCP-Cyanine5.5, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	53-7.3
Conjugate	PerCP-Cyanine5.5 APC APC-eFluor 780 Biotin eFluor 450 FITC NovaFluor Blue 610-70S NovaFluor Blue 660-120S NovaFluor Red 710 NovaFluor Red 725 NovaFluor Red 755 NovaFluor Yellow 610 NovaFluor Yellow 660 NovaFluor Yellow 755 PE PE-Cyanine7 PE-eFluor 610 Super Bright 436 Super Bright 600 Super Bright 702 Unconjugated
Excitation/Emission Max	489/679 nm View spectra
Form	Liquid
Concentration	0.2 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_914334

Product Specific Information

Description: The 53-7.3 monoclonal antibody reacts with mouse CD5, a 67 kDa protein expressed by a majority of thymocytes, mature T cells and a subset of B cells. The expression of CD5 by a small subset of B cells characterizes a developmentally and functionally distinct lineage of B cells called B-1 cells. CD5 is a counter-receptor for CD72 and plays a role in the T-B cell interaction.

Applications Tested: This 53-7.3 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD5 is a 67 kDa human T-lymphocyte single-chain transmembrane glycoprotein. CD5 is present on all mature T-lymphocytes, on most of thymocytes and on many T-cell leukemias and lymphomas. CD5 also reacts with a subpopulation of activated B-cells and may act as a receptor in regulating T-cell proliferation. CD5 is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. CD5 is expressed by many T cell leukemia, lymphomas, and activated T cells. Diseases associated with CD5 dysfunction include thymus cancer and Richter's Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

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Product References

The Influence of B Cell Depletion Therapy on Naturally Acquired Immunity to Streptococcus pneumoniae.

Frontiers in immunology

Ercoli G,Ramos-Sevillano E,Nakajima R,de Assis RR,Jasinskas A,Goldblatt D,Felgner P,Weckbecker G,Brown J

Published figure using CD5 monoclonal antibody (Product # 45-0051-82) in Flow Cytometry

Tue Jun 22 00:00:00 EDT 2021

Proteomic and bioinformatic profiling of neutrophils in CLL reveals functional defects that predispose to bacterial infections.

Blood advances

Subramaniam N,Bottek J,Thiebes S,Zec K,Kudla M,Soun C,de Dios Panal E,Lill JK,Pfennig A,Herrmann R,Bruderek K,Rahmann S,Brandau S,Johansson P,Reinhardt HC,Dürig J,Seiffert M,Bracht T,Sitek B,Engel DR

45-0051-82 was used in Flow cytometry/Cell sorting to investigate the underlying phenotypical and functional changes in neutrophils during chronic lymphocytic leukemia.

Tue Mar 09 00:00:00 EST 2021

Targeting of the Yersinia pestis F1 capsular antigen by innate-like B1b cells mediates a rapid protective response against bubonic plague.

NPJ vaccines

Levy Y,Vagima Y,Tidhar A,Aftalion M,Gur D,Nili U,Chitlaru T,Zauberman A,Mamroud E

Thu Oct 01 00:00:00 EDT 2020

Differential requirement of kindlin-3 for T cell progenitor homing to the non-vascularized and vascularized thymus.

eLife

Moretti FA,Klapproth S,Ruppert R,Margraf A,Weber J,Pick R,Scheiermann C,Sperandio M,Fässler R,Moser M

Published figure using CD5 monoclonal antibody (Product # 45-0051-82) in Flow Cytometry

Thu Sep 06 00:00:00 EDT 2018

Host Tumor Suppressor p18^INK4c Functions as a Potent Cell-Intrinsic Inhibitor of Murine Gammaherpesvirus 68 Reactivation and Pathogenesis.

Journal of virology

Niemeyer BF,Oko LM,Medina EM,Oldenburg DG,White DW,Cool CD,Clambey ET,van Dyk LF

Published figure using CD5 monoclonal antibody (Product # 45-0051-82) in Flow Cytometry

Thu Mar 15 00:00:00 EDT 2018

IgG Responses to Porins and Lipopolysaccharide within an Outer Membrane-Based Vaccine against Nontyphoidal Salmonella Develop at Discordant Rates.

mBio

Schager AE,Dominguez-Medina CC,Necchi F,Micoli F,Goh YS,Goodall M,Flores-Langarica A,Bobat S,Cook CNL,Arcuri M,Marini A,King LDW,Morris FC,Anderson G,Toellner KM,Henderson IR,López-Macías C,MacLennan CA,Cunningham AF

45-0051 was used in Flow cytometry/Cell sorting to study how antibody responses to bacterial antigens develop and how vaccines like generalised modules for membrane antigens can work and help prevent infection.

Tue Mar 06 00:00:00 EST 2018

Antibody Tumor Targeting Is Enhanced by CD27 Agonists through Myeloid Recruitment.

Cancer cell

Turaj AH,Hussain K,Cox KL,Rose-Zerilli MJJ,Testa J,Dahal LN,Chan HTC,James S,Field VL,Carter MJ,Kim HJ,West JJ,Thomas LJ,He LZ,Keler T,Johnson PWM,Al-Shamkhani A,Thirdborough SM,Beers SA,Cragg MS,Glennie MJ,Lim SH

45-0051 was used in Flow cytometry/Cell sorting to demonstrate the therapeutic advantage of using an immunomodulatory mAb to regulate lymphoid cells, which then recruit and activate myeloid cells for enhanced killing of mAb-opsonized tumors.

Mon Dec 11 00:00:00 EST 2017

Naïve helper T cells with high CD5 expression have increased calcium signaling.

PloS one

Freitas CMT,Hamblin GJ,Raymond CM,Weber KS

Published figure using CD5 monoclonal antibody (Product # 45-0051-82) in Flow Cytometry

Tue Sep 26 00:00:00 EDT 2017

CTCF-Mediated Enhancer-Promoter Interaction Is a Critical Regulator of Cell-to-Cell Variation of Gene Expression.

Molecular cell

Ren G,Jin W,Cui K,Rodrigez J,Hu G,Zhang Z,Larson DR,Zhao K

Published figure using CD5 monoclonal antibody (Product # 45-0051-82) in Flow Cytometry

Thu Sep 21 00:00:00 EDT 2017

Differential regulation of C5a receptor 1 in innate immune cells during the allergic asthma effector phase.

PloS one

Ender F,Wiese AV,Schmudde I,Sun J,Vollbrandt T,König P,Laumonnier Y,Köhl J

45-0051 was used in Flow cytometry/Cell sorting to demonstrate a complex regulation pattern of C5aR1 in the airways, lung tissue and lymph nodes of mice.

Thu Aug 17 00:00:00 EDT 2017

Two mouse models reveal an actionable PARP1 dependence in aggressive chronic lymphocytic leukemia.

Nature communications

Knittel G,Rehkämper T,Korovkina D,Liedgens P,Fritz C,Torgovnick A,Al-Baldawi Y,Al-Maarri M,Cun Y,Fedorchenko O,Riabinska A,Beleggia F,Nguyen PH,Wunderlich FT,Ortmann M,Montesinos-Rongen M,Tausch E,Stilgenbauer S,P Frenzel L,Herling M,Herling C,Bahlo J,Hallek M,Peifer M,Buettner R,Persigehl T,Reinhardt HC

45-0051 was used in Flow cytometry/Cell sorting to provide a preclinical rationale for the use of PARP inhibitors in ATM-affected human chronic lymphocytic leukemia.

Fri Jul 28 00:00:00 EDT 2017

NFATc1 supports imiquimod-induced skin inflammation by suppressing IL-10 synthesis in B cells.

Nature communications

Alrefai H,Muhammad K,Rudolf R,Pham DA,Klein-Hessling S,Patra AK,Avots A,Bukur V,Sahin U,Tenzer S,Goebeler M,Kerstan A,Serfling E

Published figure using CD5 monoclonal antibody (Product # 45-0051-82) in Flow Cytometry

Wed May 25 00:00:00 EDT 2016

Survival of Igα-Deficient Mature B Cells Requires BAFF-R Function.

Journal of immunology (Baltimore, Md. : 1950)

Levit-Zerdoun E,Becker M,Pohlmeyer R,Wilhelm I,Maity PC,Rajewsky K,Reth M,Hobeika E

45-0051 was used in Flow cytometry/Cell sorting to assess the importance of the expression of a functional B-cell receptor for B cell maintenance.

Tue Mar 01 00:00:00 EST 2016

IL-33-Dependent Group 2 Innate Lymphoid Cells Promote Cutaneous Wound Healing.

The Journal of investigative dermatology

Rak GD,Osborne LC,Siracusa MC,Kim BS,Wang K,Bayat A,Artis D,Volk SW

45-0051 was used in Flow cytometry/Cell sorting to demonstrate that cutaneous injury promotes an IL-33-dependent group 2 ILC response and that abrogation of this response can impair efficient wound closure.

Mon Feb 01 00:00:00 EST 2016

B cell Rab7 mediates induction of activation-induced cytidine deaminase expression and class-switching in T-dependent and T-independent antibody responses.

Journal of immunology (Baltimore, Md. : 1950)

Pone EJ,Lam T,Lou Z,Wang R,Chen Y,Liu D,Edinger AL,Xu Z,Casali P

Published figure using CD5 monoclonal antibody (Product # 45-0051-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2015

Group 2 innate lymphoid cells promote beiging of white adipose tissue and limit obesity.

Nature

Brestoff JR,Kim BS,Saenz SA,Stine RR,Monticelli LA,Sonnenberg GF,Thome JJ,Farber DL,Lutfy K,Seale P,Artis D

45-0051 was used in Flow cytometry/Cell sorting to study how ILC2s can regulate adipose function and metabolic homeostasis.

Thu Mar 12 00:00:00 EDT 2015

The E2 ubiquitin-conjugating enzyme UBE2J1 is required for spermiogenesis in mice.

The Journal of biological chemistry

Koenig PA,Nicholls PK,Schmidt FI,Hagiwara M,Maruyama T,Frydman GH,Watson N,Page DC,Ploegh HL

45-0051 was used in Flow cytometry/Cell sorting to identify an essential function for the ubiquitin-proteasome-system in spermiogenesis.

Fri Dec 12 00:00:00 EST 2014

Retroviral insertional mutagenesis can contribute to immortalization of mature T lymphocytes.

Molecular medicine (Cambridge, Mass.)

Newrzela S,Cornils K,Heinrich T,Schläger J,Yi JH,Lysenko O,Kimpel J,Fehse B,von Laer D

45-0051 was used in Flow cytometry/Cell sorting to investigate possible inducers of the malignant transformation of mature T lymphocytes.

Fri Aug 03 00:00:00 EDT 2012

Innate lymphoid cells promote lung-tissue homeostasis after infection with influenza virus.

Nature immunology

Monticelli LA,Sonnenberg GF,Abt MC,Alenghat T,Ziegler CG,Doering TA,Angelosanto JM,Laidlaw BJ,Yang CY,Sathaliyawala T,Kubota M,Turner D,Diamond JM,Goldrath AW,Farber DL,Collman RG,Wherry EJ,Artis D

45-0051 was used in Flow cytometry/Cell sorting to demonstrate a critical role for lung ILCs in restoring airway epithelial integrity and tissue homeostasis after viral replication.

Tue Nov 01 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: CD antigen CD5; CD5; CD5 antigen (p56 62); CD5 antigen p56-62; fCD5; Ly-1; Ly12; LyA; Lymphocyte antigen 1; Pan T cell; T-cell surface glycoprotein CD5

Gene Aliases: Cd5; Ly-1; Ly-12; Ly-A; Lyt-1

UniProt ID: (Mouse) P13379

Entrez Gene ID: (Mouse) 12507

Function(s)

T cell costimulation apoptotic signaling pathway

Process(es)

glycoprotein binding scavenger receptor activity protein binding

CD5 Monoclonal Antibody (53-7.3), PerCP-Cyanine5.5, eBioscience™

Product Details

45-0051-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

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Bioinformatics

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