|Tested species reactivity||Rat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG2a, kappa|
|Storage buffer||PBS, pH 7.2, with 0.1% gelatin|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, store in dark, DO NOT FREEZE!|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.25 µg/test|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 3 publications below|
Description: The HIS47 monoclonal antibody reacts with rat CD5, a 67 kDa surface protein expressed by a majority of thymocytes and mature T cells. While mouse and human CD5 is also expressed on a small subset of B cells called B-1 cells, such CD5^+ B cell subset has not been identified in the rat so far. CD5 is a counter-receptor for CD72 and plays a role in the T-B cell interaction.
Applications Reported: This HIS47 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This HIS47 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser
CD5 is a 67kDa human T-lymphocyte single-chain transmembrane glycoprotein. It is present on all mature T-lymphocytes, on most of thymocytes and on many T-cell leukemias and lymphomas. It also reacts with a subpopulation of activated B-cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||Long-term survival of hamster-to-rat heart xenografts based on mechanisms of accommodation and tolerance of CD5 B cells.||Lin Y,Goebels J,Vandeputte M,Waer M||Transplantation proceedings (29:null)||1997|
|Not Applicable||Not Cited||MRC OX19 recognizes the rat CD5 surface glycoprotein, but does not provide evidence for a population of CD5bright B cells.||Vermeer LA,de Boer NK,Bucci C,Bos NA,Kroese FG,Alberti S||European journal of immunology (24:585)||1994|
|Not Applicable||Not Cited||Molecular cloning of the rat CD5 gene.||Vermeer L,Bos N,Kroese F,Bucci C,Alberti S||Annals of the New York Academy of Sciences (651:82)||1992|