|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1/IgG2a|
|Immunogen||human T-acute lymphoblastic leukemia (ALL) cells and LB lymphoblastoid cell line|
|Conjugate||FITC , PE|
|Storage buffer||PBS with 0.2% carrier protein|
|Contains||0.08% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is a monoclonal mixture of FITC-conjugated CD5 (clone M28623, isotype IgG2a) and PE-conjugated CD20 (clone DFA-7, isotype IgG1).
In humans, CD5 is a 67kDa T lymphocyte single chain transmembrane glycoprotein. It is present on all mature T lymphocytes, on most of thymocytes and on many T cell leukemias and lymphomas. It reacts with a subpopulation of activated B cells. The CD20 antigen is present on human pre-B lymphocytes and on B-lymphocytes atall stage of maturation, except on plasma cells. Low level expression of the CD20 antigen has been detected on normal T lymphocytes. CD20 is a non glycosylated protein with a molecular weight of 35 or 37 kDa depending on the degree of phosphorylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.