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Invitrogen
CD50 (ICAM-3) Monoclonal Antibody (CBR-IC3/1), eBioscience™
Product Details
BMS111
| Applications | Tested Dilution | Publications |
|---|---|---|
Immunohistochemistry (IHC) |
Assay-Dependent | - |
Immunocytochemistry (ICC/IF) |
- | View 1 publication 1 publication |
Flow Cytometry (Flow) |
Assay-Dependent | - |
ELISA (ELISA) |
Assay-Dependent | - |
Functional Assay (FN) |
Assay-Dependent | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human |
Published species |
Human |
Host/Isotype |
Mouse / IgG1 |
Class |
Monoclonal |
Type |
Antibody |
Clone |
CBR-IC3/1 |
Immunogen |
Rec. Human ICAM-3 (CD50) |
Conjugate |
Unconjugated
|
Form |
Liquid |
Concentration |
0.5 mg/mL |
Purification |
Affinity chromatography |
Storage buffer |
PBS, pH 7.2-7.4 |
Contains |
no preservative |
Storage conditions |
4° C |
RRID |
AB_10597746 |
Product Specific Information
Description: Intercellular Adhesion Molecule-3 (ICAM-3) is a member of the immunoglobulin supergene family and functions as a ligand for the Lymphocyte Function-Associated Antigen-1 (LFA-1). ICAM-3 is a heavily glycosylated, integral membrane protein with five immunoglobulin-like domains. It shares high homology to ICAM-1 and ICAM-2 in the extracellular region. In contrast to ICAM-1 and ICAM-2, ICAM-3 is absent on endothelia. ICAM-3 is expressed on resting lymphocytes, monocytes and neutrophils, representing the major LFA-1 ligand on these cells. The finding that adhesion of resting T lymphocytes to LFA-1 occurs primarily via ICAM-3 combined with the fact that ICAM-3 is much better expressed than other LFA-1 ligands on monocytes and resting lymphocytes implies an important role for ICAM-3 in the initiation of immune responses.
Applications Tested: ELISA, Flow Cytometry, Functional Studies (Blocking), Immunohistochemistry.
Purity: >95%.
Target Information
CD50 (intracellular adhesion molecule 3, ICAM-3) is a transmembrane glycoprotein expressed by leukocytes, that serves as a counter-receptor for the lymphocyte function-associated antigen (LFA)-1 integrin. Besides functioning as an adhesive molecule that mediates e.g. the contact between T cells and antigen presenting cells, ICAM-3 regulates affinity of LFA-1 for ICAM-1 and induces T cell activation and proliferation. ICAM-3 plays an essential role in the initiation of the immune response both on T cells and antigen presenting cells and interacts also with CD209 (dendritic cell-specific ICAM-3-grabbing nonintegrin, DC-SIGN), a C-type lectin of dendritic cells and macrophages; this process is involved in dialogue between dendritic cells and granulocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD50; CDw50; ICAM-3; ICAM-R; Intercellular adhesion molecule 3; intercellular adhesion molecule-3
Gene Aliases: CD50; CDW50; ICAM-R; ICAM3
UniProt ID: (Human) P32942
Entrez Gene ID: (Human) 3385
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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