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Invitrogen
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
Applications | Tested Dilution | Publications |
---|---|---|
Flow Cytometry (Flow) |
Assay-Dependent | - |
Product Specifications | |
---|---|
Species Reactivity |
Human |
Host/Isotype |
Mouse / IgG1 |
Recommended Isotype Control |
Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™ |
Class |
Monoclonal |
Type |
Antibody |
Clone |
CBR-IC3/1 |
Conjugate |
FITC
|
Excitation/Emission Max |
498/517 nm View spectra |
Form |
Liquid |
Concentration |
10 µL/Test |
Purification |
Affinity chromatography |
Storage buffer |
TBS, pH 7.2-7.4, with 1% BSA |
Contains |
0.02% sodium azide |
Storage conditions |
4° C, store in dark |
RRID |
AB_10597745 |
Description: Intercellular Adhesion Molecule-3 (ICAM-3) is a member of the immunoglobulin supergene family and functions as a ligand for the Lymphocyte Function-Associated Antigen-1 (LFA-1). ICAM-3 is a heavily glycosylated, integral membrane protein with five immunoglobulin-like domains. It shares high homology to ICAM-1 and ICAM-2 in the extracellular region. In contrast to ICAM-1 and ICAM-2, ICAM-3 is absent on endothelia. ICAM-3 is expressed on resting lymphocytes, monocytes and neutrophils, representing the major LFA-1 ligand on these cells. The finding that adhesion of resting T lymphocytes to LFA-1 occurs primarily via ICAM-3 combined with the fact that ICAM-3 is much better expressed than other LFA-1 ligands on monocytes and resting lymphocytes implies an important role for ICAM-3 in the initiation of immune responses.
Applications Tested: Flow Cytometry.
Purity: >95%.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser
CD50 (intracellular adhesion molecule 3, ICAM-3) is a transmembrane glycoprotein expressed by leukocytes, that serves as a counter-receptor for the lymphocyte function-associated antigen (LFA)-1 integrin. Besides functioning as an adhesive molecule that mediates e.g. the contact between T cells and antigen presenting cells, ICAM-3 regulates affinity of LFA-1 for ICAM-1 and induces T cell activation and proliferation. ICAM-3 plays an essential role in the initiation of the immune response both on T cells and antigen presenting cells and interacts also with CD209 (dendritic cell-specific ICAM-3-grabbing nonintegrin, DC-SIGN), a C-type lectin of dendritic cells and macrophages; this process is involved in dialogue between dendritic cells and granulocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Protein Aliases: CD50; CDw50; ICAM-3; ICAM-R; Intercellular adhesion molecule 3; intercellular adhesion molecule-3
Gene Aliases: CD50; CDW50; ICAM-R; ICAM3
UniProt ID: (Human) P32942
Entrez Gene ID: (Human) 3385
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