Invitrogen

CD51/CD61 Monoclonal Antibody (23C6)

View all (7) CD51/CD61 antibodies

Questions & Answers

Questions & Answers

Product Details

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

-

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

Flow Cytometry (Flow)

1:40-1:80

-

Immunoprecipitation (IP)

Assay-dependent

-

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Chicken, Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

23C6

Immunogen

Osteoclasts from osteoclastomas

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

4°C or -20°C if preferred

RRID

AB_2537984

Product Specific Information

This antibody does not react with bovine, pig or rabbit.

The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommend the use of acetone fixation for frozen sections. For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Target Information

CD51 and CD61 molecules (alpha V and beta 3 integrins) form a complex. This complex binds vitronection at the RGD sequence and can also bind fibrinogen, von Willebrand factor, thrombospondin, fibronectin, osteopontin and collagen. Reacts with osteoclasts, placenta, melanoma cell lines and weakly with platelets.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: antigen CD61; antigen identified by monoclonal antibody L230; CD51; CD61; GPIIIa; integrin alpha V; integrin alpha-5 beta-3; Integrin alpha-V; Integrin alpha-V heavy chain; Integrin alpha-V light chain; integrin alphaVbeta3; Integrin beta; integrin beta 3; Integrin beta-3; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61); Platelet membrane glycoprotein IIIa; Vitronectin receptor; vitronectin receptor alpha subunit; Vitronectin receptor subunit alpha

Gene Aliases: BDPLT16; BDPLT2; CD51; CD61; GP3A; GPIIIa; GT; integrin; ITGAV; ITGB3; MSK8; VNRA; VTNR

UniProt ID: (Human) P06756, (Chicken) P26008, (Human) P05106

Entrez Gene ID: (Human) 3685, (Chicken) 396420, (Human) 3690, (Chicken) 374209

Molecular Function: integrin

angiogenesis antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent cell adhesion cell-matrix adhesion integrin-mediated signaling pathway positive regulation of cell proliferation negative regulation of macrophage derived foam cell differentiation negative regulation of lipid storage cell growth cell migration extracellular matrix organization positive regulation of cell migration cell-substrate adhesion negative regulation of lipid transport positive regulation of osteoblast proliferation heterotypic cell-cell adhesion substrate adhesion-dependent cell spreading endodermal cell differentiation apolipoprotein A-I-mediated signaling pathway apoptotic cell clearance negative regulation of low-density lipoprotein particle receptor biosynthetic process positive regulation of cell adhesion viral entry into host cell vascular endothelial growth factor receptor signaling pathway negative regulation of lipoprotein metabolic process regulation of phagocytosis leukocyte migration negative chemotaxis entry of symbiont into host cell by promotion of host phagocytosis ERK1 and ERK2 cascade calcium ion transmembrane transport extrinsic apoptotic signaling pathway in absence of ligand regulation of apoptotic cell clearance negative regulation of entry of bacterium into host cell negative regulation of extrinsic apoptotic signaling pathway cell adhesion mediated by integrin regulation of transforming growth factor beta activation positive regulation of protein phosphorylation positive regulation of endothelial cell proliferation platelet degranulation cell-substrate junction assembly blood coagulation positive regulation of endothelial cell migration smooth muscle cell migration platelet activation positive regulation of vascular endothelial growth factor receptor signaling pathway activation of protein kinase activity tube development wound healing regulation of bone resorption mesodermal cell differentiation positive regulation of peptidyl-tyrosine phosphorylation angiogenesis involved in wound healing platelet aggregation

Process(es)

virus receptor activity opsonin binding fibronectin binding protease binding protein kinase C binding voltage-gated calcium channel activity protein binding coreceptor activity fibroblast growth factor binding C-X3-C chemokine binding insulin-like growth factor I binding neuregulin binding metal ion binding transforming growth factor beta binding extracellular matrix binding extracellular matrix protein binding protein disulfide isomerase activity platelet-derived growth factor receptor binding enzyme binding identical protein binding vascular endothelial growth factor receptor 2 binding cell adhesion molecule binding