Description: The 23C6 monoclonal antibody reacts with the human CD51/CD61 dimer, also known as the integrin alphav/beta3. CD51, an ~120 kDa surface molecule can also non-covalently associate with other beta subunits of the integrin family including beta1 (CD29), beta5 and beta6 to form receptors for extracellular matrix components. Heterodimers of CD51/CD61 are expressed by melanoma cells, endothelial cells and osteoclasts and at very low levels by platelets. The CD51/CD61 complex mediates adhesion to fibrinogen, fibronectin, vitronectin and thrombospondin.
Applications Reported: The 23C6 antibody has been reported for use in flow cytometric analysis.
Applications Tested: The 23C6 antibody has been pre-titrated and tested by flow cytometric analysis of human melanoma cell line and peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
CD51 and CD61 molecules (alpha V and beta 3 integrins) form a complex. This complex binds vitronection at the RGD sequence and can also bind fibrinogen, von Willebrand factor, thrombospondin, fibronectin, osteopontin and collagen. Reacts with osteoclasts, placenta, melanoma cell lines and weakly with platelets.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: antigen CD61; antigen identified by monoclonal antibody L230; CD51; CD61; GPIIIa; integrin alpha V; integrin alpha-5 beta-3; Integrin alpha-V; Integrin alpha-V heavy chain; Integrin alpha-V light chain; integrin alphaVbeta3; integrin beta 3; Integrin beta-3; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61); Platelet membrane glycoprotein IIIa; Vitronectin receptor; Vitronectin receptor subunit alpha
Gene Aliases: BDPLT16; BDPLT2; CD51; CD61; GP3A; GPIIIa; GT; ITGAV; ITGB3; MSK8; VNRA; VTNR
Molecular Function: integrin