Invitrogen

CD54 (ICAM-1) Monoclonal Antibody (YN1/1.7.4), FITC, eBioscience™

Product Image Gallery

CD54 (ICAM-1) Antibody in Flow Cytometry (Flow) — Staining of BALB/c splenocytes with staining buffer (autofluorescence) (open histogram) or 0.5 µg of Anti-Mouse CD54 (ICAM-1) FITC (filled histogram). Total viable cells were used for analysis.

CD54 (ICAM-1) Antibody in Immunocytochemistry (ICC/IF) — Figure 3 PAT inhibition suppresses leucocyte adhesion and ICAM-1 expression on endothelial surface during inflammatory stimulation. ( a , b ) Leucocyte-endothelium interactions were assessed via intravital microscopic analysis of mesenteric microcirculation in rats subjected to SIRS elicited by bacterial lipopolysaccharide (LPS, 10 mg kg -1 , IP, 4 h). A group of animals received intravenous 2-BP (2 mg kg -1 ) immediately after LPS injection. Leucocytes were labelled with acridine orange. ( a ) Representative images of leucocyte adhesion in postcapillary venules. Scale bars, 30 mum. ( b ) Quantification of leucocyte slow-rolling flux (number of cells per min), slow-rolling fraction (% of slow rolling cells to total rolling cells), rolling velocity and adhesion. Increased slow-rolling flux or decreased rolling velocity indicates the likelihood of firm adhesion. Data are presented as mean+-s.e.m. from 4 rats with >=10 venules per group. * P <0.05 versus sham+vehicle, # P <0.05 versus SIRS+vehicle. ( c , d ) The effects of 2-BP on human leucocyte adhesion to human umbilical vein endothelial cells with or without stimulation by IL-1beta (100 ng ml -1 , 4 h). 2-BP (10 muM) or vehicle was given simultaneously with IL-1beta. ( c ) Representative images of adherent leucocytes (green) on endothelial cells (red) with nuclei stained with DAPI. Embedded images show green channel (leucocytes) alone. Scale bars, 25 mum. ( d ) Quantification of adherent leucocytes to IL-1beta-stimulated ECs

CD54 (ICAM-1) Antibody in Neutralization (Neu) — Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 11-0541-82) in Neutralization

Product Details

11-0541-82

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	1 µg/test	View 15 publications 15 publications
Neutralization (Neu)	-	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	-	View 2 publications 2 publications
Immunohistochemistry (Frozen) (IHC (F))	-	View 1 publication 1 publication
Immunohistochemistry (IHC)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Mouse
Published species	Mouse
Host/Isotype	Rat / IgG2b, kappa
Recommended Isotype Control	Rat IgG2b kappa Isotype Control (eB149/10H5), FITC, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	YN1/1.7.4
Conjugate	FITC Biotin Functional Grade PE Unconjugated
Excitation/Emission Max	498/517 nm View spectra
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_465094

Product Specific Information

Description: The YN1/1.7.4 monoclonal antibody reacts with mouse CD54 (InterCellular Adhesion Molecule-1, ICAM-1), a 90-110 kDa transmembrane glycoprotein expressed by monocytes, lymphocytes, dendritic cells, and endothelial cells. Expression of CD54 is upregulated on activated lymphocytes. Interaction of CD54 with its ligands CD11a and CD11b is important in the inflammatory response. The YN1/1.7.4 monoclonal antibody recognizes a different epitope than the eBioKAT-1 monclonal antibody.

Applications Reported: The YN1/1.7.4 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The YN1/1.7.4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

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Product References

Cell-permeable transgelin-2 as a potent therapeutic for dendritic cell-based cancer immunotherapy.

Journal of hematology & oncology

Kim HR,Park JS,Park JH,Yasmin F,Kim CH,Oh SK,Chung IJ,Jun CD

11-0541 was used in Flow cytometry/Cell sorting to indicate that cell-permeable transgelin-2 have a potential clinical value as a cancer immunotherapy based on DCs.

Wed Mar 17 00:00:00 EDT 2021

Interaction between ICAM1 in endothelial cells and LFA1 in T cells during the pathogenesis of experimental Parkinson's disease.

Experimental and therapeutic medicine

Li W,Chen S,Luo Y,Xia Y,Ma Q,Yao Q,Wu J

11-0541 was used in Flow cytometry/Cell sorting to investigate the infiltration of T cells in lesions in the central nervous system, in patients with and without Parkinson's Disease.

Sat Aug 01 00:00:00 EDT 2020

Conserved regulation of RNA processing in somatic cell reprogramming.

BMC genomics

Kanitz A,Syed AP,Kaji K,Zavolan M

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 11-0541-82) in Flow Cytometry

Thu Jan 31 00:00:00 EST 2019

Low-Dose Radiotherapy Has No Harmful Effects on Key Cells of Healthy Non-Inflamed Joints.

International journal of molecular sciences

Deloch L,Rückert M,Fietkau R,Frey B,Gaipl US

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 11-0541-82) in Flow Cytometry

Tue Oct 16 00:00:00 EDT 2018

PAI1 mediates fibroblast-mast cell interactions in skin fibrosis.

The Journal of clinical investigation

Pincha N,Hajam EY,Badarinath K,Batta SPR,Masudi T,Dey R,Andreasen P,Kawakami T,Samuel R,George R,Danda D,Jacob PM,Jamora C

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 11-0541-82) in Neutralization

Tue May 01 00:00:00 EDT 2018

Constitutively Active SMAD2/3 Are Broad-Scope Potentiators of Transcription-Factor-Mediated Cellular Reprogramming.

Cell stem cell

Ruetz T,Pfisterer U,Di Stefano B,Ashmore J,Beniazza M,Tian TV,Kaemena DF,Tosti L,Tan W,Manning JR,Chantzoura E,Ottosson DR,Collombet S,Johnsson A,Cohen E,Yusa K,Linnarsson S,Graf T,Parmar M,Kaji K

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 11-0541-82) in Flow Cytometry

Thu Dec 07 00:00:00 EST 2017

Cyclic AMP-Elevating Capacity of Adenylate Cyclase Toxin-Hemolysin Is Sufficient for Lung Infection but Not for Full Virulence of Bordetella pertussis.

Infection and immunity

Skopova K,Tomalova B,Kanchev I,Rossmann P,Svedova M,Adkins I,Bibova I,Tomala J,Masin J,Guiso N,Osicka R,Sedlacek R,Kovar M,Sebo P

11-0541 was used in Flow cytometry/Cell sorting to construct the first B. pertussis mutant secreting a CyaA toxin having an intact capacity to deliver the AC enzyme into CD11b-expressing host phagocytes but impaired in formation of cell-permeabilizing pores and defective in cAMP elevation in CD11b- cells.

Thu Jun 01 00:00:00 EDT 2017

Palmitoyl acyltransferase DHHC21 mediates endothelial dysfunction in systemic inflammatory response syndrome.

Nature communications

Beard RS,Yang X,Meegan JE,Overstreet JW,Yang CG,Elliott JA,Reynolds JJ,Cha BJ,Pivetti CD,Mitchell DA,Wu MH,Deschenes RJ,Yuan SY

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 11-0541-82) in Immunofluorescence

Thu Sep 22 00:00:00 EDT 2016

Reprogramming Roadblocks Are System Dependent.

Stem cell reports

Chantzoura E,Skylaki S,Menendez S,Kim SI,Johnsson A,Linnarsson S,Woltjen K,Chambers I,Kaji K

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 11-0541-82) in Flow Cytometry

Tue Sep 08 00:00:00 EDT 2015

MicroRNA-155 Exerts Cell-Specific Antiangiogenic but Proarteriogenic Effects During Adaptive Neovascularization.

Circulation

Pankratz F,Bemtgen X,Zeiser R,Leonhardt F,Kreuzaler S,Hilgendorf I,Smolka C,Helbing T,Hoefer I,Esser JS,Kustermann M,Moser M,Bode C,Grundmann S

11-0541 was used in Flow cytometry/Cell sorting to identify regulatory microRNAs involved in the modulation of neovascularisation after femoral artery occlusion in mice.

Tue May 05 00:00:00 EDT 2015

Anti-inflammatory functions of protein C require RAGE and ICAM-1 in a stimulus-dependent manner.

Mediators of inflammation

Braach N,Buschmann K,Pflaum J,Hudalla H,Koch L,Ryschich E,Poeschl J,Frommhold D

11-0541 was used in Flow cytometry/Cell sorting to show that RAGE and ICAM-1 are cooperatively involved in protein C-induced inhibition of leukocyte recruitment.

Sat Feb 07 00:00:00 EST 2015

Dietary gluten increases natural killer cell cytotoxicity and cytokine secretion.

European journal of immunology

Larsen J,Dall M,Antvorskov JC,Weile C,Engkilde K,Josefsen K,Buschard K

11-0541 was used in Flow cytometry/Cell sorting to study the in vitro and in vivo effects of gluten on natural killer cells.

Wed Oct 01 00:00:00 EDT 2014

Elongated TCR alpha chain CDR3 favors an altered CD4 cytokine profile.

BMC biology

Reynolds C,Chong D,Raynsford E,Quigley K,Kelly D,Llewellyn-Hughes J,Altmann D,Boyton R

11-0541 was used in Flow cytometry/Cell sorting to study how the conformation of TCR alpha chain CDR3 favours different CD4 cytokine profiles.

Fri May 09 00:00:00 EDT 2014

High-resolution analysis with novel cell-surface markers identifies routes to iPS cells.

Nature

O'Malley J,Skylaki S,Iwabuchi KA,Chantzoura E,Ruetz T,Johnsson A,Tomlinson SR,Linnarsson S,Kaji K

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 11-0541-82) in Flow Cytometry

Thu Jul 04 00:00:00 EDT 2013

Host DNA released in response to aluminum adjuvant enhances MHC class II-mediated antigen presentation and prolongs CD4 T-cell interactions with dendritic cells.

Proceedings of the National Academy of Sciences of the United States of America

McKee AS,Burchill MA,Munks MW,Jin L,Kappler JW,Friedman RS,Jacobelli J,Marrack P

11-0541 was used in Flow cytometry/Cell sorting to study how aluminium acts as an adjuvant by introducing host DNA into the cytoplasm of dendritic cells, where it engages receptors promoting MHC II presentation.

Tue Mar 19 00:00:00 EDT 2013

Ontogeny of stromal organizer cells during lymph node development.

Journal of immunology (Baltimore, Md. : 1950)

Bénézech C,White A,Mader E,Serre K,Parnell S,Pfeffer K,Ware CF,Anderson G,Caamaño JH

11-0541 was used in Immunofluorescence to highlight the importance of the signals and cellular interactions that induce the maturation of stromal cells.

Thu Apr 15 00:00:00 EDT 2010

Inflammatory regulation by TLR3 in acute hepatitis.

Journal of immunology (Baltimore, Md. : 1950)

Xiao X,Zhao P,Rodriguez-Pinto D,Qi D,Henegariu O,Alexopoulou L,Flavell RA,Wong FS,Wen L

11-0541 was used in Immunohistochemistry to observe a novel role for TLR3 in hepatocyte damage in the absence of a viral stimulus in a murine model of hepatitis.

Tue Sep 15 00:00:00 EDT 2009

In vivo expansion of T reg cells with IL-2-mAb complexes: induction of resistance to EAE and long-term acceptance of islet allografts without immunosuppression.

The Journal of experimental medicine

Webster KE,Walters S,Kohler RE,Mrkvan T,Boyman O,Surh CD,Grey ST,Sprent J

11-0541 was used in Flow cytometry/Cell sorting to study the role of T reg cells in the induction of resistance to EAE and long term acceptance of islet allografts.

Mon Apr 13 00:00:00 EDT 2009

Noninvasive imaging of dendritic cell migration into lymph nodes using near-infrared fluorescent semiconductor nanocrystals.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Noh YW,Lim YT,Chung BH

11-0541 was used in Immunocytochemistry-immunoflourescence to establish the potential of noninvasive in vivo imaging of near-infrared-emitting quantum dots for tracking immunotherapeutic cells.

Sat Nov 01 00:00:00 EDT 2008

Two-dimensional kinetics regulation of alphaLbeta2-ICAM-1 interaction by conformational changes of the alphaL-inserted domain.

The Journal of biological chemistry

Zhang F,Marcus WD,Goyal NH,Selvaraj P,Springer TA,Zhu C

11-0541 was used in Flow cytometry/Cell sorting to quantify the conformational regulation of the interaction between ICAM-1 and integrin α(L)β(2), or its inserted domain in isolation, expressed on K562 cells.

Fri Dec 23 00:00:00 EST 2005

Bioinformatics

Protein Aliases: CD54; Human rhinovirus receptor; ICAM-1; Intercellular adhesion molecule 1; Ly 47; MALA-2; MALA2; MyD10; sCD54; soluble CD54; Surface antigen of activated B cells

Gene Aliases: CD54; Icam-1; Icam1; Ly-47; MALA-2

UniProt ID: (Mouse) P13597

Entrez Gene ID: (Mouse) 15894

Function(s)

Process(es)

integrin binding protein complex binding

CD54 (ICAM-1) Monoclonal Antibody (YN1/1.7.4), FITC, eBioscience™

Product Details

11-0541-82

Flow Cytometry (Flow)

Neutralization (Neu)

Immunocytochemistry (ICC/IF)

Immunohistochemistry (Frozen) (IHC (F))

Immunohistochemistry (IHC)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

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