|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Storage buffer||PBS, pH 7.4|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:10-1:25|
|Immunohistochemistry (Frozen) (IHC (F))||1:100-1:1000|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul. This antibody is not recommend for staining erythrocytes in flow cytometry applications. A suggested positive control for immunohistochemical applications is human tonsil.
This gene encodes a protein involved in the regulation of the complement cascade. The encoded glycoprotein is also known as the decay-accelerating factor (DAF); binding of DAF to complement proteins accelerates their decay, disrupting the cascade and preventing damage to host cells. Antigens present on the DAF glycoprotein constitute the Cromer blood group system (CROM). Two alternatively spliced transcripts encoding different proteins have been identified. The predominant transcript encodes a membrane-bound protein expressed on cells exposed to plasma component proteins but an alternatively spliced transcript produces a soluble protein present at much lower levels. Additional, alternatively spliced transcript variants have been described, but their biological validity has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Guidelines for the diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria and related disorders by flow cytometry.
MA1-82424 was used in flow cytometry to discuss ways to standardize flow cytometry methods to diagnose paroxysmal nocturnal hemoglobinuria
|Borowitz MJ,Craig FE,Digiuseppe JA,Illingworth AJ,Rosse W,Sutherland DR,Wittwer CT,Richards SJ||Cytometry. Part B, Clinical cytometry (78:211)||2010|