Invitrogen

CD56 Monoclonal Antibody (56C04)

Product Image Gallery

CD56 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of CD56 (green) showing staining in the cytoplasm and membrane of SH-SY5Y cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD56 monoclonal antibody (Product # MA5-11563) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

CD56 Antibody in Immunohistochemistry (IHC) — Formalin-fixed, paraffin-embedded human neuroblastoma stained with CD56 antibody using peroxidase-conjugate and AEC chromogen. Note cell membrane staining of tumor cells.

CD56 Antibody in Western Blot (WB) — Western blot analysis of CD56 was performed by loading 25 µg of SK-N-MC cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with a CD56 monoclonal antibody (Product # MA5-11563) at a dilution of 1:200 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at approx. 140 kDa.

CD56 Antibody in Flow Cytometry (Flow) — Flow cytometry analysis of CD56 in PBMC cells (green) compared to an isotype control (blue). Human blood was collected, combined with a hydrophilic polysaccharide, centrifuged, transferred to a conical tube and washed with PBS. 50 µL of cell solution was added to each tube at a dilution of 2x10^7 cells/mL, followed by the addition of 50 µL of isotype control and primary antibody (Product # MA5-11563) at a dilution of 0.5 µg/test. Cells were incubated for 30 min at 4°C and washed with a cell buffer, followed by incubation with a DyLight 488-conjugated goat anti-mouse IgG (H+L) secondary for 30 min at 4°C in the dark. FACS analysis was performed using 400 µL of cell buffer.

Product Details

Tested Applications

Dilution

Flow Cytometry (Flow)

0.5 µg/test

Immunocytochemistry (ICC)

1:10-1:200

Immunofluorescence (IF)

1:10-1:200

Immunohistochemistry (Paraffin) (IHC (P))

1-2 µg/mL

Western Blot (WB)

1:100-1:500

Published Applications

Immunohistochemistry (IHC)

See 6 publications below

Immunohistochemistry (Paraffin) (IHC (P))

See 1 publication below

Product Specifications

Species Reactivity

Human, Rat

Published species

Human

Host / Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

56C04

Immunogen

Membrane preparation of a small cell lung carcinoma

Conjugate

Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

RRID

AB_10985168

Product Specific Information

MA5-11563 targets CD56 in IHC (P), Western blot, ICC/IF and FACS applications and shows reactivity with Human and Rat samples.

The MA5-11563 immunogen is membrane preparation of a small cell lung carcinoma.

Target Information

Three isoforms of neural cell adhesion molecule (NCAM) are produced by differential splicing of the RNA transcript from a single gene. The 135kDa isoform is the basic molecule which is glycosylated or sialylated to produce the mature species. NCAM (CD56) is reported to express on most neuroectodermal derived cell lines, tissues, and neoplasms such as retinoblastoma, medullblastoma, astrocytoma, and neuroblastoma. It is also expressed on some mesodermally derived tumors such as rhabdomyosarcoma and also on natural killer cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: antigen recognized by monoclonal antibody 5.1H11; CD-56; CD56; MSK39; N-CAM-1; NCAM; NCAM1; Neural cell adhesion molecule 1; neural cell adhesion molecule, NCAM

Gene Aliases: CD56; MSK39; N-CAM; N-CAM-1; NCAM; NCAM-1; NCAM-C; NCAM1; NCAMC

UniProt ID: (Human) P13591, (Rat) P13596

Entrez Gene ID: (Human) 4684, (Rat) 24586

Molecular Function: cell adhesion molecule cytokine receptor defense/immunity protein hydrolase immunoglobulin receptor superfamily immunoglobulin superfamily cell adhesion molecule phosphatase protein phosphatase receptor