Invitrogen

CD56 Monoclonal Antibody (56C04)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details

7 References

View all (123) CD56 (NCAM) antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

CD56 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of CD56 was performed using 70% confluent log phase SH-SY5Y and HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CD56 Monoclonal Antibody (56C04) (Product # MA5-11563) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing localization to plasma membrane. Panel e shows HEK-293 cells with no expression of CD56. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

CD56 Antibody in Immunohistochemistry (IHC) — Formalin-fixed, paraffin-embedded human neuroblastoma stained with CD56 antibody using peroxidase-conjugate and AEC chromogen. Note cell membrane staining of tumor cells.

CD56 Antibody in Western Blot (WB) — Western blot was performed using Anti-CD56 (NCAM) Monoclonal Antibody (56C04), (Product # MA5-11563) and ~150 kDa band corresponding to NCAM was observed across cell lines tested except in MCF7, HepG2, Neuro2a and PC12 which are reported to be negative. Whole cell extracts (30 µg lysate) of SHSY5Y (Lane 1), IMR32 (Lane 2), NK-92 (Lane 3), MCF7 (Lane 4), HepG2 (Lane 5), Neuro2a (Lane 6) and PC12 (Lane 7) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blots were probed with the primary antibody (4 µg/mL) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP conjugate (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Super Signal™ West Dura Extended Duration Substrate (Product # 34076).

CD56 Antibody in Flow Cytometry (Flow) — Flow cytometry analysis of CD56 in PBMC cells (green) compared to an isotype control (blue). Human blood was collected, combined with a hydrophilic polysaccharide, centrifuged, transferred to a conical tube and washed with PBS. 50 µL of cell solution was added to each tube at a dilution of 2x10^7 cells/mL, followed by the addition of 50 µL of isotype control and primary antibody (Product # MA5-11563) at a dilution of 0.5 µg/test. Cells were incubated for 30 min at 4°C and washed with a cell buffer, followed by incubation with a DyLight 488-conjugated goat anti-mouse IgG (H+L) secondary for 30 min at 4°C in the dark. FACS analysis was performed using 400 µL of cell buffer.

CD56 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines and tissues owing to their inherent genetic constitution. Relative expression of NCAM was observed in SHSY5Y, IMR-32, NK-92, when compared to MCF7, HepG2, Neuro2a and PC12 using CD56 (NCAM) Monoclonal Antibody (56C04), (Product # MA5-11563). {RE}

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:500

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1-2 µg/mL

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:10-1:200

Flow Cytometry (Flow)

0.5 µg/test

Product Specifications

Species Reactivity

Human, Rat

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

56C04

Immunogen

Membrane preparation of a small cell lung carcinoma

Conjugate

Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

RRID

AB_10985168

Product Specific Information

MA5-11563 targets CD56 in IHC (P), Western blot, ICC/IF and FACS applications and shows reactivity with Human and Rat samples.

The MA5-11563 immunogen is membrane preparation of a small cell lung carcinoma.

Target Information

CD56 (NCAM, neural cell adhesion molecule) is a transmembrane glycoprotein of the immunoglobulin family that serves as an adhesive molecule and is ubiquitously expressed in the nervous system in isoforms ranging from 120-180 kDa. CD56 is found on T cells and NK cells, and is involved in cell migration, axonal growth, pathfinding and synaptic plasticity. Polysialic modification results in reduction of CD56-mediated cell adhesion. Through its extracellular region, CD56 mediates homophilic and heterophilic interactions by binding extracellular matrix components such as laminin and integrins. CD56 is expressed on most neuroectodermal derived cell lines, tissues and neoplasms such as retinoblastoma, medulloblastoma, astrocytomas and neuroblastoma. Further, CD56 is a widely used neuroendocrine marker with a high sensitivity for neuroendocrine tumors and ovarian granulosa cell tumors. Diseases associated with CD56 dysfunction include rabies and blastic plasmacytoid dendritic cell neoplasms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: antigen recognized by monoclonal antibody 5.1H11; CD-56; CD56; E NCAM; N CAM1; N-CAM-1; neural cell adhesion molecule; Neural cell adhesion molecule 1; neural cell adhesion molecule, NCAM; sCD56; sNCAM; soluble CD56; soluble NCAM

Gene Aliases: CD56; MSK39; N-CAM; N-CAM-1; NCAM; NCAM-1; NCAM-C; NCAM1; NCAMC

UniProt ID: (Human) P13591, (Rat) P13596

Entrez Gene ID: (Human) 4684, (Rat) 24586

Function(s)