Invitrogen

CD56 Monoclonal Antibody (56C04)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

1 Published Figure

8 References

View all (137) CD56 (NCAM) antibodies

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Datasheet

Protocols

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CD56 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of CD56 was performed using 70% confluent log phase SH-SY5Y and HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CD56 Monoclonal Antibody (56C04) (Product # MA5-11563) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing localization to plasma membrane. Panel e shows HEK-293 cells with no expression of CD56. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

CD56 Antibody in Immunohistochemistry (IHC) — Formalin-fixed, paraffin-embedded human neuroblastoma stained with CD56 antibody using peroxidase-conjugate and AEC chromogen. Note cell membrane staining of tumor cells.

CD56 Antibody in Western Blot (WB) — Western blot was performed using Anti-CD56 (NCAM) Monoclonal Antibody (56C04), (Product # MA5-11563) and ~150 kDa band corresponding to NCAM was observed across cell lines tested except in MCF7, HepG2, Neuro2a and PC12 which are reported to be negative. Whole cell extracts (30 µg lysate) of SHSY5Y (Lane 1), IMR32 (Lane 2), NK-92 (Lane 3), MCF7 (Lane 4), HepG2 (Lane 5), Neuro2a (Lane 6) and PC12 (Lane 7) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blots were probed with the primary antibody (4 µg/mL) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP conjugate (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Super Signal™ West Dura Extended Duration Substrate (Product # 34076).

CD56 Antibody in Flow Cytometry (Flow) — Flow cytometry analysis of CD56 in PBMC cells (green) compared to an isotype control (blue). Human blood was collected, combined with a hydrophilic polysaccharide, centrifuged, transferred to a conical tube and washed with PBS. 50 µL of cell solution was added to each tube at a dilution of 2x10^7 cells/mL, followed by the addition of 50 µL of isotype control and primary antibody (Product # MA5-11563) at a dilution of 0.5 µg/test. Cells were incubated for 30 min at 4°C and washed with a cell buffer, followed by incubation with a DyLight 488-conjugated goat anti-mouse IgG (H+L) secondary for 30 min at 4°C in the dark. FACS analysis was performed using 400 µL of cell buffer.

CD56 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines and tissues owing to their inherent genetic constitution. Relative expression of NCAM was observed in SHSY5Y, IMR-32, NK-92, when compared to MCF7, HepG2, Neuro2a and PC12 using CD56 (NCAM) Monoclonal Antibody (56C04), (Product # MA5-11563). {RE}

Product Details

MA5-11563

Applications	Tested Dilution	Publications
Western Blot (WB)	1:100-1:500	-
Immunohistochemistry (IHC)	-	View 7 publications 7 publications
Immunohistochemistry (Paraffin) (IHC (P))	1-2 µg/mL	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	1:10-1:200	-
Flow Cytometry (Flow)	0.5 µg/test	-

Product Specifications
Species Reactivity	Human, Rat
Published species	Human
Host/Isotype	Mouse / IgG1, kappa
Class	Monoclonal
Type	Antibody
Clone	56C04
Immunogen	Membrane preparation of a small cell lung carcinoma
Conjugate	Unconjugated
Form	Liquid
Concentration	0.2 mg/mL
Purification	Protein G
Storage buffer	PBS, pH 7.4, with 0.2% BSA
Contains	0.09% sodium azide
Storage conditions	4° C
RRID	AB_10985168

Product Specific Information

MA5-11563 targets CD56 in IHC (P), Western blot, ICC/IF and FACS applications and shows reactivity with Human and Rat samples.

The MA5-11563 immunogen is membrane preparation of a small cell lung carcinoma.

Target Information

CD56 (NCAM, neural cell adhesion molecule) is a transmembrane glycoprotein of the immunoglobulin family that serves as an adhesive molecule and is ubiquitously expressed in the nervous system in isoforms ranging from 120-180 kDa. CD56 is found on T cells and NK cells, and is involved in cell migration, axonal growth, pathfinding and synaptic plasticity. Polysialic modification results in reduction of CD56-mediated cell adhesion. Through its extracellular region, CD56 mediates homophilic and heterophilic interactions by binding extracellular matrix components such as laminin and integrins. CD56 is expressed on most neuroectodermal derived cell lines, tissues and neoplasms such as retinoblastoma, medulloblastoma, astrocytomas and neuroblastoma. Further, CD56 is a widely used neuroendocrine marker with a high sensitivity for neuroendocrine tumors and ovarian granulosa cell tumors. Diseases associated with CD56 dysfunction include rabies and blastic plasmacytoid dendritic cell neoplasms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Induction of muscle-regenerative multipotent stem cells from human adipocytes by PDGF-AB and 5-azacytidine.

Science advances

Yeola A,Subramanian S,Oliver RA,Lucas CA,Thoms JAI,Yan F,Olivier J,Chacon D,Tursky ML,Srivastava P,Potas JR,Hung T,Power C,Hardy P,Ma DD,Kilian KA,McCarroll J,Kavallaris M,Hesson LB,Beck D,Curtis DJ,Wong JWH,Hardeman EC,Walsh WR,Mobbs R,Chandrakanthan V,Pimanda JE

Published figure using CD56 monoclonal antibody (Product # MA5-11563) in Immunohistochemistry

Fri Jan 01 00:00:00 EST 2021

Tumor immune microenvironment characterization in clear cell renal cell carcinoma identifies prognostic and immunotherapeutically relevant messenger RNA signatures.

Genome biology

Şenbabaoğlu Y,Gejman RS,Winer AG,Liu M,Van Allen EM,de Velasco G,Miao D,Ostrovnaya I,Drill E,Luna A,Weinhold N,Lee W,Manley BJ,Khalil DN,Kaffenberger SD,Chen Y,Danilova L,Voss MH,Coleman JA,Russo P,Reuter VE,Chan TA,Cheng EH,Scheinberg DA,Li MO,Choueiri TK,Hsieh JJ,Sander C,Hakimi AA

MA511563 was used in immunohistochemistry to profile the infiltration levels of immune cell populations in 19 cancer types

Thu Nov 17 00:00:00 EST 2016

Early reversal cells in adult human bone remodeling: osteoblastic nature, catabolic functions and interactions with osteoclasts.

Histochemistry and cell biology

Abdelgawad ME,Delaisse JM,Hinge M,Jensen PR,Alnaimi RW,Rolighed L,Engelholm LH,Marcussen N,Andersen TL

MA511563 was used in immunohistochemistry - paraffin section to show that reversal cells colonizing bone surfaces right after resorption are osteoblast-lineage cells

Wed Jun 01 00:00:00 EDT 2016

Clinicopathological characteristics of kidney mucinous tubular and spindle cell carcinoma.

International journal of clinical and experimental pathology

Chen Q,Gu Y,Liu B

MA5-11563 was used in Immunohistochemistry to characterise, clinicopathologically, two cases of kidney mucinous tubular and spindle cell carcinoma.

Wed Nov 25 00:00:00 EST 2015

Increased presence of capillaries next to remodeling sites in adult human cancellous bone.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Kristensen HB,Andersen TL,Marcussen N,Rolighed L,Delaisse JM

MA5-11563 was used in immunohistochemistry to study whether cancellous bone remodeling is associated with vascularization

Fri Mar 01 00:00:00 EST 2013

Pigmented paraganglioma of the kidney: a case report.

Diagnostic pathology

Zhao L,Luo J,Zhang H,Da J

MA5-11563 was used in immunohistochemistry to report on a case of pigmented paraganglioma of the kidney

Thu Jun 28 00:00:00 EDT 2012

Hepatosplenic gammadelta T-cell lymphoma with ring chromosome 7, an isochromosome 7q equivalent clonal chromosomal aberration.

Virchows Archiv : an international journal of pathology

Tamaska J,Adam E,Kozma A,Gopcsa L,Andrikovics H,Tordai A,Halm G,Bereczki L,Bagdi E,Krenacs L

MA5-11563 was used in immunohistochemistry to investigate the ring chromosome 7 in hepatosplenic gamma-delta T cell lymphoma

Sun Oct 01 00:00:00 EDT 2006

Isolated idiopathic bile ductular hyperplasia in patients with persistently abnormal liver function tests.

Journal of hepatology

Sonzogni A,Colloredo G,Fabris L,Cadamuro M,Paris B,Roffi L,Pozzi M,Bovo G,Del Poggio P,Portmann BC,Strazzabosco M

MA5-11563 was used in immunohistochemistry to characterize idiopathic isolated ductular hyperplasia in patients undergoing liver biopsy

Thu Apr 01 00:00:00 EST 2004

Bioinformatics

Protein Aliases: antigen recognized by monoclonal antibody 5.1H11; CD-56; CD56; E NCAM; N CAM1; N-CAM-1; neural cell adhesion molecule; Neural cell adhesion molecule 1; neural cell adhesion molecule, NCAM; sCD56; sNCAM; soluble CD56; soluble NCAM

Gene Aliases: CD56; MSK39; N-CAM; N-CAM-1; NCAM; NCAM-1; NCAM-C; NCAM1; NCAMC

UniProt ID: (Human) P13591, (Rat) P13596

Entrez Gene ID: (Human) 4684, (Rat) 24586

Function(s)