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          • Primary Antibodies ›
          • CD56 (NCAM) Antibodies

          Invitrogen

          CD56 Monoclonal Antibody (56C04)

          Advanced Verification

          This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

          1 Published Figure
          9 References
          View all (163) CD56 (NCAM) antibodies
          Datasheet
          Protocols
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          Tech Support

          Cite CD56 Monoclonal Antibody (56C04)

          Datasheet
          Protocols
          Questions & Answers
          Tech Support
          • Antibody Testing Data (10)
          • Published Figures (1)
          • Advanced Verification (2)
          CD56 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 13

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          CD56 Antibody (MA5-11563) in WB

          Western blot was performed using Anti-CD56 (NCAM) Monoclonal Antibody (56C04), (Product # MA5-11563) and ~150 kDa band corresponding to NCAM was observed across cell lines tested except in MCF7, HepG2, Neuro2a and PC12 which are reported to be negative. Whole cell extracts (30 µg lysate) of SHSY5Y (Lane 1), IMR32 (Lane 2), NK-92 (Lane 3), MCF7 (Lane 4), HepG2 (Lane 5), Neuro2a (Lane 6) and PC12 (Lane 7) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitroc... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Product Details

          MA5-11563

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:100-1:500
          -

          Immunohistochemistry (IHC)

          -
          View 8 publications 8 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1-2 µg/mL
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          1:10-1:200
          -

          Flow Cytometry (Flow)

          0.5 µg/test
          -
          Product Specifications

          Species Reactivity

          Human, Rat

          Published species

          Human

          Host/Isotype

          Mouse / IgG1, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          56C04

          Immunogen

          Membrane preparation of a small cell lung carcinoma

          Conjugate

          Unconjugated

          Form

          Liquid

          Concentration

          0.2 mg/mL

          Purification

          Protein A/G

          Storage buffer

          PBS, pH 7.4, with 0.05% BSA

          Contains

          0.05% sodium azide

          Storage conditions

          4° C

          RRID

          AB_10985168

          Product Specific Information

          MA5-11563 targets CD56 in IHC (P), Western blot, ICC/IF and FACS applications and shows reactivity with Human and Rat samples.

          The MA5-11563 immunogen is membrane preparation of a small cell lung carcinoma.

          Target Information

          CD56 (NCAM, neural cell adhesion molecule) is a transmembrane glycoprotein of the immunoglobulin family that serves as an adhesive molecule and is ubiquitously expressed in the nervous system in isoforms ranging from 120-180 kDa. CD56 is found on T cells and NK cells, and is involved in cell migration, axonal growth, pathfinding and synaptic plasticity. Polysialic modification results in reduction of CD56-mediated cell adhesion. Through its extracellular region, CD56 mediates homophilic and heterophilic interactions by binding extracellular matrix components such as laminin and integrins. CD56 is expressed on most neuroectodermal derived cell lines, tissues and neoplasms such as retinoblastoma, medulloblastoma, astrocytomas and neuroblastoma. Further, CD56 is a widely used neuroendocrine marker with a high sensitivity for neuroendocrine tumors and ovarian granulosa cell tumors. Diseases associated with CD56 dysfunction include rabies and blastic plasmacytoid dendritic cell neoplasms.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: antigen recognized by monoclonal antibody 5.1H11; CD-56; CD56; E NCAM; N CAM1; N-CAM-1; neural cell adhesion molecule; Neural cell adhesion molecule 1; neural cell adhesion molecule, NCAM; sCD56; sNCAM; soluble CD56; soluble NCAM

          View more View less

          Gene Aliases: CD56; MSK39; N-CAM; N-CAM-1; NCAM; NCAM-1; NCAM-C; NCAM1; NCAMC

          View more View less

          UniProt ID: (Human) P13591, (Rat) P13596

          View more View less

          Entrez Gene ID: (Human) 4684, (Rat) 24586

          View more View less

          Function(s)
          MAPK cascade cell adhesion axon guidance positive regulation of GTPase activity viral entry into host cell regulation of synaptic plasticity interferon-gamma-mediated signaling pathway regulation of exocyst assembly cell surface receptor signaling pathway aging learning or memory response to inorganic substance response to lead ion peripheral nervous system axon regeneration response to fluoxetine calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules thalamus development organ regeneration neuron projection development multicellular organismal response to stress homotypic cell-cell adhesion response to cocaine neuron development positive regulation of calcium-mediated signaling regulation of sensory perception of pain positive regulation of cardiac muscle cell proliferation negative regulation of cell death cellular response to molecule of bacterial origin cellular response to inorganic substance
          Process(es)
          virus receptor activity Ras guanyl-nucleotide exchange factor activity fibroblast growth factor receptor binding protein binding cytoskeletal protein binding heparin binding phosphatase binding LRR domain binding identical protein binding
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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