Immunofluorescent analysis of CD56 (green) showing staining in the cytoplasm and membrane of SH-SY5Y cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD56 monoclonal antibody (Product # MA5-11563) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Human, Rat|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Membrane preparation of a small cell lung carcinoma|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.5 ug/test|
|Immunohistochemistry (Paraffin) (IHC (P))||1-2 µg/ml|
|Western Blot (WB)||1:100-1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 4 publications below|
MA5-11563 targets CD56 in IHC (P), Western blot, ICC/IF and FACS applications and shows reactivity with Human and Rat samples.
The MA5-11563 immunogen is membrane preparation of a small cell lung carcinoma.
Three isoforms of neural cell adhesion molecule (NCAM) are produced by differential splicing of the RNA transcript from a single gene. The 135kDa isoform is the basic molecule which is glycosylated or sialylated to produce the mature species. NCAM (CD56) is reported to express on most neuroectodermal derived cell lines, tissues, and neoplasms such as retinoblastoma, medullblastoma, astrocytoma, and neuroblastoma. It is also expressed on some mesodermally derived tumors such as rhabdomyosarcoma and also on natural killer cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Increased presence of capillaries next to remodeling sites in adult human cancellous bone.
MA5-11563 was used in immunohistochemistry to study whether cancellous bone remodeling is associated with vascularization
|Kristensen HB,Andersen TL,Marcussen N,Rolighed L,Delaisse JM||Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research (28:574)||2013|
Pigmented paraganglioma of the kidney: a case report.
MA5-11563 was used in immunohistochemistry to report on a case of pigmented paraganglioma of the kidney
|Zhao L,Luo J,Zhang H,Da J||Diagnostic pathology (7:null)||2012|
Hepatosplenic gammadelta T-cell lymphoma with ring chromosome 7, an isochromosome 7q equivalent clonal chromosomal aberration.
MA5-11563 was used in immunohistochemistry to investigate the ring chromosome 7 in hepatosplenic gamma-delta T cell lymphoma
|Tamaska J,Adam E,Kozma A,Gopcsa L,Andrikovics H,Tordai A,Halm G,Bereczki L,Bagdi E,Krenacs L||Virchows Archiv : an international journal of pathology (449:479)||2006|
Isolated idiopathic bile ductular hyperplasia in patients with persistently abnormal liver function tests.
MA5-11563 was used in immunohistochemistry to characterize idiopathic isolated ductular hyperplasia in patients undergoing liver biopsy
|Sonzogni A,Colloredo G,Fabris L,Cadamuro M,Paris B,Roffi L,Pozzi M,Bovo G,Del Poggio P,Portmann BC,Strazzabosco M||Journal of hepatology (40:592)||2004|