|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||CD56 positive cells.|
|Storage buffer||PBS, pH 7.4|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:10-1:500|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:500|
|Immunoprecipitation (IP)||0.5-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Suggested positive control: antigen standard for NCAM1 (transient overexpression lysate).
NCAM, as a member of the immunoglobulin superfamily of adhesion molecules is characterized by several immunoglobulin (Ig) like domains. The extracellular part of NCAM consists of five of these Ig domains and two fibronectin type III homology regions. NCAM is encoded by a single copy gene composed of 26 exons. However, at least 20-30 distinct isoforms can be generated by alternative splicing and by posttranslational modifications, such as sialylation. During sialylation, polysialic acid (PSA) carbohydrates are attached to the extracellular part of NCAM. Through its extracellular region, NCAM mediates homophilic interactions. In addition, NCAM can also undergo heterophilic interactions by binding extracellular matrix components, such as laminin, or other cell adhesion molecules, such as integrins. NCAM is expressed on most neuroectodermal derived cell lines, tissues and neoplasm such as retinoblastoma, medulloblastoma, astrocytomas and neuroblastoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Cell stress molecules in the skeletal muscle of GNE myopathy.
MA1-46055 was used in immunohistochemistry to study the overexpression of alpha-B crystallin and iNOS in GNE myopathy and the significance for disease pathogenesis
|Fischer C,Kleinschnitz K,Wrede A,Muth I,Kruse N,Nishino I,Schmidt J||BMC neurology (13:null)||2013|
Proinflammatory cell stress in sporadic inclusion body myositis muscle: overexpression of alphaB-crystallin is associated with amyloid precursor protein and accumulation of beta-amyloid.
MA1-46055 was used in immunohistochemistry to investigate the level of alphaB-crystallin in sporadic inclusion body myositis muscle
|Muth IE,Barthel K,Bähr M,Dalakas MC,Schmidt J||Journal of neurology, neurosurgery, and psychiatry (80:1344)||2009|
HHV8-positive, EBV-positive Hodgkin lymphoma-like large B-cell lymphoma and HHV8-positive intravascular large B-cell lymphoma.
MA1-46055 was used in immunohistochemistry to investigate pathologic features of two clinical cases of large B-cell lymphoma with human herpesvirus type 8
|Ferry JA,Sohani AR,Longtine JA,Schwartz RA,Harris NL||Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc (22:618)||2009|
Provision of an explanation for the inefficacy of immunotherapy in sporadic inclusion body myositis: quantitative assessment of inflammation and ß-amyloid in the muscle.
MA1-46055 was used in immunocytochemistry to study the mechanisms underlying the lack of efficacy of intravenous immunoglobulin and prednisone in sporadic inclusion body myositis
|Zschüntzsch J,Voss J,Creus K,Sehmisch S,Raju R,Dalakas MC,Schmidt J||Arthritis and rheumatism (64:4094)||2012|
Administration of betaHCG leads to dose-dependent changes of gene expression signature of endometriotic stromal cells.
MA1-46055 was used in immunocytochemistry to study the effect of human chorionic gonadotrophin on the expression profile of endometriotic stromal cells
|Hudelist G,Huber A,Auer M,Knöfler M,Saleh L,Hrachowitz C,Huber JC,Kubista E,Manavi M,Bilban M,Singer CF||Reproductive biomedicine online (20:699)||2010|