|Tested species reactivity||Human, Non-human primate|
|Published species reactivity||Mouse, Human, Not Applicable|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Tissue / cell preparation (KG-1 human acute myelogenous leukaemia cell line).|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||15mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-21343 detects NCAM (Phycoerythrin) in Human and monkey samples.
MA1-21343 has been successfully used in FACS procedures. Use 20 µl of reagent per 100 µl whole blood.
MA1-21343 immunogen corresponds to tissue / cell preparation (KG-1 human acute myelogenous leukemia cell line).
Store at 4°C short term. For extended storage, aliquot and store at -20°C, avoiding freeze/thaw cycles.
NCAM, as a member of the immunoglobulin superfamily of adhesion molecules is characterized by several immunoglobulin (Ig)-like domains. The extracellular part of NCAM consists of five of these Ig domains and two fibronectin type III homology regions. NCAM is encoded by a single copy gene composed of 26 exons. However, at least 20-30 distinct isoforms can be generated by alternative splicing and by posttranslational modifications, such as sialylation. During sialylation, polysialic acid (PSA) corbohydrates are attached to the extracellular part of NCAM. Through its extracellular region, NCAM mediates homophilic interactions. In addition, NCAM can also undergo heterophilic interactions by binding extracellular matrix components, such as laminin, or other cell adhesion molecules, such as integrins. NCAM is expressed on most neuroectodermal derived cell lines, tissues and neoplasm such as retinoblastoma, medulloblastoma, astrocytomas and neuroblastoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Grb10 deletion enhances muscle cell proliferation, differentiation and GLUT4 plasma membrane translocation.
MA1-21343 was used in flow cytometry to study the increased muscle cell differentiation/proliferation and plama membrane translocation of Glut4 in mice deleted for Grb10
|Mokbel N,Hoffman NJ,Girgis CM,Small L,Turner N,Daly RJ,Cooney GJ,Holt LJ||Journal of cellular physiology (229:1753)||2014|
|Not Applicable||Not Cited||
Isolation of large numbers of mesenchymal stem cells from the washings of bone marrow collection bags: characterization of fresh mesenchymal stem cells.
MA1-21343 was used in flow cytometry to test if the normally discarded bone marrow collection kits are a convenient source of large numbers of mesenchymal stem cells
|Mageed AS,Pietryga DW,DeHeer DH,West RA||Transplantation (83:1019)||2007|
The endogenous danger signals HSP70 and MICA cooperate in the activation of cytotoxic effector functions of NK cells.
MA1-21343 was used in flow cytometry to report that HSP70 activates human NK cells to kill target cells expressing MICA in a natural killer group 2 member D-dependent manner
|Elsner L,Flügge PF,Lozano J,Muppala V,Eiz-Vesper B,Demiroglu SY,Malzahn D,Herrmann T,Brunner E,Bickeböller H,Multhoff G,Walter L,Dressel R||Journal of cellular and molecular medicine (14:992)||2010|