|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified recombinant fragment of human CD59 (amino acids: 31-111) expressed in E. Coli.|
|Storage buffer||PBS with 0.5% proprietary stabilizer|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:200 - 1:400|
|Immunocytochemistry (ICC)||1:50 -1:200|
|Immunofluorescence (IF)||1:50 - 200|
|Immunohistochemistry (IHC)||1:200 - 1:1000|
|Western Blot (WB)||1:500 - 1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-17046 targets CD59 in FACS, ICC, IHC, IF and WB applications and shows reactivity with Human samples.
The MA5-17046 immunogen is purified recombinant fragment of human CD59 (amino acids: 31-111) expressed in E. Coli.
MA5-17046 detects CD59 which has a predicted molecular weight of approximately 14.2kDa.
This gene encodes a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. This protein also plays a role in signal transduction pathways in the activation of T cells. Mutations in this gene cause CD59 deficiency, a disease resulting in hemolytic anemia and thrombosis, and which causes cerebral infarction. Multiple alternatively spliced transcript variants, which encode the same protein, have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.