|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||1/5-1/10|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay dependent (Non-redu|
|Immunocytochemistry (ICC)||See 1 publications below|
|Miscellaneous PubMed (MISC)||See 8 publications below|
|Flow Cytometry (Flow)||See 5 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Human , Rhesus monkey|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Thymocytes and T lymphocytes.|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
In Western blot, this product recognizes CD59 under non-reducing conditions. A suggested positive control for immunohistochemical applications is tonsil. For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
CD59 is a PI-linked glycoprotein, 18-20kDa, found on all types of leucocytes including platelets. The antigen is expressed on many haemapoietic and non-haemapoietic cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 16.3A5; 1F5; 1F5 antigen; 20 kDa homologous restriction factor; CD59; CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16, EJ30, EL32 and G344); CD59 glycoprotein; CD59 molecule complement regulatory protein; CD59 molecule, complement regulatory protein; EJ16; EJ30; EL32; G344; HRF-20; HRF20; human leukocyte antigen MIC11; Ly-6-like protein; lymphocytic antigen CD59/MEM43; MAC-inhibitory protein; MAC-IP; MACIF; MEM43; MEM43 antigen; membrane attack complex (MAC) inhibition factor; Membrane attack complex inhibition factor; Membrane inhibitor of reactive lysis; MIC11; MIN1; MIN2; MIN3; MIRL; MSK21; p18-20; Protectin; surface anitgen recognized by monoclonal antibody 16.3A5; T cell-activating protein
Gene Aliases: 16.3A5; 1F5; CD59; EJ16; EJ30; EL32; G344; HRF-20; HRF20; MAC-IP; MACIF; MEM43; MIC11; MIN1; MIN2; MIN3; MIRL; MSK21; p18-20
UniProt ID: (Human) P13987
Entrez Gene ID: (Human) 966
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