Description: The MEL-14 monoclonal antibody reacts with mouse CD62L, a 76 kDa member of the selectin family. CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells and binds a number of glycosylated, fucosylated, sulfated sialylated glycoproteins including CD34, glycam-1 and MAdCam-1. These interactions mediate rolling of lymphocytes on activated endothelium at the sites of inflammation and homing of cells to the high endothelial venules (HEV) of peripheral lymphoid tissues.
Applications Reported: This MEL-14 antibody has been reported for use in flow cytometric analysis.
Our internal testing shows that Alexa Fluor 561 non-specifically stains B cells in Swiss Webster and SJL mice. Non-specific staining has not been observed in BALB/c or C57BL/6 mice. Other strains have not been tested. See the Antibody Testing Data for an example of this strain-dependent difference.
Applications Tested: This MEL-14 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Alexa Fluor 561 emits at 575 nm and is intended for use on spectral cytometers where it may be multiplexed with both PE and PE-eFluor 610.
Excitation: 558 nm; Emission: 575 nm; Laser: Yellow-Green Laser
The human CD62L is a 74-95 kDa glycoprotein member of the selectin family of adhesion receptors. L-Selectin is comprised of an aminoterminal C-type lectin binding domain, an epidermal growth factor-like domain, two short consensus repeat (SCR) sequences homologous to those found in complement binding proteins, a short spacer region, a transmembrane region and a short cytoplasmic region. Human CD62L (L-Selectin) is constitutively expressed on all classes of leukocytes including lymphocytes (except a substantial population of memory T cells), monocytes and polymorphonuclear cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CD62 antigen-like family member L; CD62L; hLHRc; L-selectin; LAM-1; LECAM1; Leu-8; Leukocyte adhesion molecule 1; Leukocyte-endothelial cell adhesion molecule 1; Ly-22; Lymph node homing receptor; Lymphocyte antigen 22; Lymphocyte surface MEL-14 antigen
Gene Aliases: AI528707; CD62L; L-selectin; LECAM-1; Lnhr; Ly-22; Ly-m22; Ly22; Lyam-1; Lyam1; Sell
UniProt ID: (Mouse) P18337
Entrez Gene ID: (Mouse) 20343