Invitrogen

CD62L (L-Selectin) Monoclonal Antibody (MEL-14), Super Bright™ 436, eBioscience™

Product Image Gallery

CD62L (L-Selectin) Antibody in Flow Cytometry (Flow) — C57BL/6 mouse splenocytes were stained with CD3e Monoclonal Antibody, APC (Product # 17-0031-82) and 0.125 µg of Rat IgG2a kappa Isotype Control, Super Bright 436 (Product # 62-4321-82) (left) or 0.125 µg of CD62L Monoclonal Antibody, Super Bright 436 (right). Cells in the lymphocyte gate were used for analysis.

CD62L (L-Selectin) Antibody in Immunocytochemistry (ICC/IF) — Fig. 4 CXCL1 recruits peripheral naive CD4 + T cells and induces their differentiation into Tregs in situ by activating the NF-kappaB/FOXP3 pathway. a The distributions of CD34 + blood vessels (red), CD62L + naive T cells (yellow), and FOXP3 + Tregs (green) within the TME of mammary tumors were detected using the tissue immunofluorescence assay. Scale bar = 5 mum. b The expression levels of CXCR2 in naive CD4 + T cells sorted from the mammary tumors were detected by flow cytometry. c Flow cytometry assay indicated that 10-30 ng/ml CXCL1 treatment for 24 h significantly increased the chemotaxis efficacy of naive CD4 + T cells, whereas CXCL1/CXCR2 blockage by 2 muM SB225002 (CXCR2 inhibitor) partially abrogated that effect. d-e The expression levels of FOXP3 in 293 T cells ( d ) and naive CD4 + T cells ( e ) when treated as indicated for 24 h were detected by Western blot assay and immunofluorescence assay, respectively. Scale bar = 3 mum. f-g The mRNA expression level ( f ) and the promoter activity of FOXP3 gene in naive CD4 + T cells when treated as indicated for 24 h were detected by qPCR and double luciferase reporter gene assay, respectively. h-i Western blot assay ( h ) and cell immunofluorescence assay ( i ) indicated that CXCL1 treatment (10-30 ng/ml) for 24 h induced the expression and nuclear translocation of NF-kappab subunit p65, while CXCL1/CXCR2 blockage partially abrogated that effect. Scale bar = 3 mum. j CHIP-PCR assay validated that CXCL1 treatment (10-30 ng/

Product Details

62-0621-82

Applications	Tested Dilution	Publications
Immunocytochemistry (ICC/IF)	-	View 1 publication 1 publication
Flow Cytometry (Flow)	0.25 µg/test	View 21 publications 21 publications

Product Specifications
Species Reactivity	Mouse
Published species	Not Applicable
Host/Isotype	Rat / IgG2a, kappa
Recommended Isotype Control	Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 436, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	MEL-14
Conjugate	Super Bright™ 436 Alexa Fluor 561 Alexa Fluor 700 APC APC-eFluor 780 Biotin Brilliant UV 395 Brilliant UV 496 Brilliant UV 615 Brilliant UV 737 Brilliant UV 805 Brilliant Violet 421 Brilliant Violet 480 Brilliant Violet 650 eFluor 450 FITC Functional Grade NovaFluor Blue 510 NovaFluor Blue 555 NovaFluor Blue 585 NovaFluor Blue 610-30S NovaFluor Blue 660-40S NovaFluor Red 710 NovaFluor Yellow 590 NovaFluor Yellow 660
Excitation/Emission Profile	View spectra
Form	Liquid
Concentration	0.2 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2, with BSA
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_2762739

Product Specific Information

Description: The MEL-14 monoclonal antibody reacts with mouse CD62L, a 76 kDa member of the selectin family. CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells and binds a number of glycosylated, fucosylated, sulfated sialylated glycoproteins including CD34, glycam-1 and MAdCam-1. These interactions mediate rolling of lymphocytes on activated endothelium at the sites of inflammation and homing of cells to the high endothelial venules (HEV) of peripheral lymphoid tissues.

Applications Reported: This MEL-14 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This MEL-14 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

The human CD62L is a 74-95 kDa glycoprotein member of the selectin family of adhesion receptors. L-Selectin is comprised of an aminoterminal C-type lectin binding domain, an epidermal growth factor-like domain, two short consensus repeat (SCR) sequences homologous to those found in complement binding proteins, a short spacer region, a transmembrane region and a short cytoplasmic region. Human CD62L (L-Selectin) is constitutively expressed on all classes of leukocytes including lymphocytes (except a substantial population of memory T cells), monocytes and polymorphonuclear cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

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Product References

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Liu J,Liu Y,Panda S,Liu A,Lei J,Burd I

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Nature communications

Li H,Wang M,Huang B,Zhu SW,Zhou JJ,Chen DR,Cui R,Zhang M,Sun ZJ

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Cell reports

Cannons JL,Villarino AV,Kapnick SM,Preite S,Shih HY,Gomez-Rodriguez J,Kaul Z,Shibata H,Reilley JM,Huang B,Handon R,McBain IT,Gossa S,Wu T,Su HC,McGavern DB,O'Shea JJ,McGuire PJ,Uzel G,Schwartzberg PL

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Frontiers in cell and developmental biology

Hao C,Wang W,Zhan B,Wang Z,Huang J,Sun X,Zhu X

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Nature communications

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Bioinformatics

Protein Aliases: CD62 antigen-like family member L; CD62L; hLHRc; L-selectin; LAM-1; LECAM1; Leu-8; Leukocyte adhesion molecule 1; Leukocyte-endothelial cell adhesion molecule 1; Ly-22; Lymph node homing receptor; Lymphocyte antigen 22; Lymphocyte surface MEL-14 antigen

Gene Aliases: AI528707; CD62L; L-selectin; LECAM-1; Lnhr; Ly-22; Ly-m22; Ly22; Lyam-1; Lyam1; Sell

UniProt ID: (Mouse) P18337

Entrez Gene ID: (Mouse) 20343

Function(s)

cell adhesion response to ATP regulation of apoptotic process positive regulation of neutrophil chemotaxis

Process(es)

protease binding protein binding carbohydrate binding cell adhesion molecule binding glycolipid binding