Staining of Thrombin-activated human platelets with Anti-Human CD41a FITC (Product # 11-0419) and 0.125 µg of Mouse IgG1 K Isotype Control PE (Product # 12-4714) (left) or 0.125 µg of Anti-Human/Mouse CD62P (P-Selectin) PE (right). Total viable cells were used for analysis.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG1, kappa|
|Storage buffer||PBS, pH 7.2, with 0.1% gelatin|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, store in dark, DO NOT FREEZE!|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.25 µg/test|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Description: The Psel.KO2.3 monoclonal antibody reacts with mouse CD62P (also known as P-selectin and GMP-140), a 140-kDa cell adhesion molecule that can be induced on endothelial cells and platelets. During inflammation, this selectin mediates adhesion of monocytes and neutrophils to activated platelets, as well as leukocyte rolling on endothelial cells. The major ligand for CD62P on neutrophils is P-selectin glycoprotein ligand-1 (PSGL-1). This monoclonal antibody recognizes an epitope that is conserved across several species (e.g. mouse, human, and rat) and has not been reported to block cell adhesion.
Applications Reported: This Psel.KO2.3 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This Psel.KO2.3 antibody was tested by flow cytometric analysis on thrombin-activated human platelets. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
P-selectin is a 140 kDa type 1 transmembrane glycoprotein, and is one of the most commonly studied proteins that regulate cell rolling. P-selectin is stored in the Weibel-Palade bodies of endothelial cells, as well as in a-granules of platelets. From there, it can be rapidly brought to the cell surface after exposure to thrombin, histamine, complement 5a, Ca21 ionophores, or adenosine diphosphate.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.