This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details
Description: This X54-5/7.1 monoclonal antibody recognizes allelic variants a and b of mouse CD64, a high affinity Fc binding receptor also known as Fc-gammaR1 or Fcgr1. Alloform a is present in the following mouse strains: C57BL/6, BALB/c, DBA/2, and alloform b in strains: C3H/HeJ, CBA/J, NZW, SJL/J, 129/SvJ. In addition this antibody has been reported positive in strains: AKR, ALR, BUB, C58, CE, HRS,MRL, MON, NZB, NZO, NZW, PL, SJL, ST and SWR. This clone X54-5/7.1 will not recognize the allelic variant d, present in NOD/Lt mice. This antibody has been reported to stain RAW264.7 cell line.
Applications Reported: This X54-5/7.1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This X54-5/7.1 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
APC-eFluor™ 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochrome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser
CD64 (FCGR1A) encodes a protein that plays an important role in the immune response. CD64 is a high-affinity Fc-gamma receptor and is one of three related gene family members located on chromosome 1. CD64 plays an important role in the immune response and its dysfunction has been studied in cervical adenitis and tetrasomy 21.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CD64; CD64 antigen; Fc-gamma receptor 1; Fc-gamma RI; FcgRI; FcRI; FLJ18345; High affinity immunoglobulin gamma Fc receptor I; IgG Fc receptor I; IgG high affinity Fc receptor; RP11-196G18.2
Gene Aliases: AI323638; AV092959; CD64; Fcg1; FcgammaRI; Fcgr1; IGGHAFC
UniProt ID: (Mouse) P26151
Entrez Gene ID: (Mouse) 14129
Molecular Function: cell adhesion molecule