Invitrogen

CD68 Monoclonal Antibody (KP1), eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

12 Published Figures

20 References

View all (116) CD68 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

CD68 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of CD68 was performed using 70% confluent log phase THP-1 cells differentiated into Macrophage (M0 phase). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CD68 Mouse Monoclonal Antibody (KP1) (Product # 14-0688-80) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membrane localization. Panel e shows untreated cells with no signal. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

CD68 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry on formalin-fixed paraffin embedded human cerebellar white matter, using 1 µg/mL of Mouse IgG1 Isotype Control (left) or 1 µg/mL of Anti-Human CD68 Purified (right) followed by biotinylated Anti-Mouse IgG, and DAB visualization. Nuclei are counterstained with hematoxylin.

CD68 Antibody — Altered expression of target protein upon cell treatment demonstrates antibody specificity. Immunofluorescence analysis of CD68 using Anti-CD68 Mouse Monoclonal Antibody (KP1) (Product # 14-0688-80) shows increased expression of proteins in THP-1 cell line upon differentiation into Macrophage (M0 phase). {TM}

CD68 Antibody in Immunohistochemistry (IHC) — Figure 6 Immuno-Histo-Chemistry (IHC) in 37 human adenocarcinoma samples. ( A ) Staining scored blinded by a pathologist at Pantomics, Inc. Staining > 2.8 was considered strong DUOX2 positive, score of ""0"" was considered DUOX2 negative. Percentage of strong staining and negative DUOX2 staining are indicated. ( B ) Example of heterogeneous (positive and negative) DUOX2 staining in well differentiated/low grade gastric adenocarcinoma (Malignant 1). Red arrows indicate cells expressing DUOX2. ( C ) Expanded view of inset from ( B ). ( D ) Example of strong DUOX2 staining in poorly differentiated/high grade stomach adenocarcinoma. ( E ) Intensity of CD68 staining in DUOX2 negative and positive samples. Student's t -test, *** p = 0.0005. ( F ) CD68, red arrows indicate macrophages tumor and stroma infiltration in same tissue as ( B ) expressing DUOX2. ( G ) Expanded view of inset from ( F ). ( H ) IHC in poorly differentiated/high grade stomach adenocarcinoma showing strong CD68 staining. Images dimensions are 450 x 383 pixels (W x H).

CD68 Antibody in Flow Cytometry (Flow) — Published figure using CD68 monoclonal antibody (Product # 14-0688-82) in Flow Cytometry

Product Details

14-0688-82

Applications	Tested Dilution	Publications
Western Blot (WB)	Assay-Dependent	-
Immunohistochemistry (IHC)	-	View 9 publications 9 publications
Immunohistochemistry (Paraffin) (IHC (P))	1 µg/mL	View 2 publications 2 publications
Immunohistochemistry (PFA fixed) (IHC (PFA))	-	View 1 publication 1 publication
Immunohistochemistry (Frozen) (IHC (F))	1 µg/mL	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	5 µg/ml	View 3 publications 3 publications
Flow Cytometry (Flow)	Assay-Dependent	View 4 publications 4 publications
Immunoprecipitation (IP)	Assay-Dependent	-

Product Specifications
Species Reactivity	Human
Published species	Human, Mouse
Host/Isotype	Mouse / IgG1, kappa
Class	Monoclonal
Type	Antibody
Clone	KP1
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C
RRID	AB_11151139

Product Specific Information

Description: The KP1 antibody reacts with human CD68 which belongs to the sialomucin family and is closely related to the family of lysosomal-associated membrane proteins (lamps) and scavenger receptor. CD68 is predominantly an intracellular protein, found mainly in the late endosomal compartment but can be detected in smaller amounts on the surface of mainly myeloid-derived cells; monocytes and macrophages, dendritic cells (and in some langerhan cells), neutrophils, basophils, mast cells, myeloid progenitor cells, and a subset of CD34+ hematopoietic bone marrow progenitor cells. The function has not been fully elucidated but based on homology and structure, CD68 may play a role in antigen processing or presentation and protection of the lysosomal membrane from hydrolytic enzymes. Reports have also shown expression in activated T cells, and about 40% of peripheral blood B-lymphocytes and 50% of all B-ALL. The KP1 antibody has also been reported to stain fibroblasts which may be due to a conserved epitope rather than fibroblasts expressing CD68.

Applications Reported: This KP1 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, western blotting, immunohistochemical staining of frozen tissue sections, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.

Applications Tested: This KP1 antibody has been tested by immunohistochemistry with both low and high pH antigen retrieval on FFPE tissue. Either antigen retrieval method can be used. This antibody can be used at less than or equal to 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD68 (Macrosialin) is a 110 kDa integral membrane glycoprotein predominantly expressed on the intracellular lysomsomes of monocytes and macrophages and to a lesser extent by dendritic cells and peripheral blood granulocytes. Also, CD68 could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. CD68 is expressed by interdigitating reticulum cells in tonsil and some histiocytic lymphoma or histiocytosis, acute myeloid leukemia (AML), and granulocytic sarcoma. Elevated expression of CD68 has been demonstrated on CD34+ cells in various human malignancies, including several Acute Myeloid Leukemia studies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CNS delivery of anti-CD52 antibodies modestly reduces disease severity in an animal model for multiple sclerosis.

Therapeutic advances in chronic disease

Bogie JF,Grajchen E,Wouters E,Broux B,Stinissen P,Van Wijmeersch B,Hendriks JJ

14-0688-82 was used in Immunocytochemistry to study whether central nervous system (CNS) delivery of anti-CD52 antibodies reduces disease severity and the neuroinflammatory burden in the experimental autoimmune encephalomyelitis (EAE) model.

Sat Apr 16 00:00:00 EDT 2022

DUOX2, a New Biomarker for Disseminated Gastric Cancer's Response to Low Dose Radiation in Mice.

Cancers

Parekh PR,Solano-Gonzalez E,Martins MB,Ma X,Tighe K,Casildo A,Zodda A,Johnstone C,Poirier Y,Mahmood J,Bhalla K,Li S,Lapidus RG,Carrier F

Published figure using CD68 monoclonal antibody (Product # 14-0688-82) in Immunohistochemistry

Fri Aug 20 00:00:00 EDT 2021

Sialic acid-binding immunoglobulin-like lectin-15 expression on peritumoral macrophages is a favorable prognostic factor for primary central nervous system lymphoma patients.

Scientific reports

Fudaba H,Momii Y,Hirakawa T,Onishi K,Asou D,Matsushita W,Kawasaki Y,Sugita K,Fujiki M

Published figure using CD68 monoclonal antibody (Product # 14-0688-82) in Immunocytochemistry

Wed Jan 13 00:00:00 EST 2021

Sialic acid-binding immunoglobulin-like lectin-15 expression on peritumoral macrophages is a favorable prognostic factor for primary central nervous system lymphoma patients.

Scientific reports

Fudaba H,Momii Y,Hirakawa T,Onishi K,Asou D,Matsushita W,Kawasaki Y,Sugita K,Fujiki M

Published figure using CD68 monoclonal antibody (Product # 14-0688-82) in Immunocytochemistry

Wed Jan 13 00:00:00 EST 2021

Modeling COVID-19 with Human Pluripotent Stem Cell-Derived Cells Reveals Synergistic Effects of Anti-inflammatory Macrophages with ACE2 Inhibition Against SARS-CoV-2.

Research square

Duan F,Guo L,Yang L,Han Y,Thakur A,Nilsson-Payant BE,Wang P,Zhang Z,Ma CY,Zhou X,Han T,Zhang T,Wang X,Xu D,Duan X,Xiang J,Tse HF,Liao C,Luo W,Huang FP,Chen YW,Evans T,Schwartz RE,tenOever B,Ho DD,Chen S,Lian Q,Chen HJ

Published figure using CD68 monoclonal antibody (Product # 14-0688-82) in Immunohistochemistry

Thu Aug 20 00:00:00 EDT 2020

Modeling COVID-19 with Human Pluripotent Stem Cell-Derived Cells Reveals Synergistic Effects of Anti-inflammatory Macrophages with ACE2 Inhibition Against SARS-CoV-2.

Research square

Published figure using CD68 monoclonal antibody (Product # 14-0688-82) in Immunohistochemistry

Thu Aug 20 00:00:00 EDT 2020

CD36-mediated uptake of myelin debris by macrophages and microglia reduces neuroinflammation.

Journal of neuroinflammation

Grajchen E,Wouters E,van de Haterd B,Haidar M,Hardonnière K,Dierckx T,Van Broeckhoven J,Erens C,Hendrix S,Kerdine-Römer S,Hendriks JJA,Bogie JFJ

14-0688 was used in Flow cytometry/Cell sorting to define the mRNA and protein abundance of CD36 in myelin-containing phagocytes.

Mon Jul 27 00:00:00 EDT 2020

Interplay between Liver X Receptor and Hypoxia Inducible Factor 1α Potentiates Interleukin-1β Production in Human Macrophages.

Cell reports

Ménégaut L,Thomas C,Jalil A,Julla JB,Magnani C,Ceroi A,Basmaciyan L,Dumont A,Le Goff W,Mathew MJ,Rébé C,Dérangère V,Laubriet A,Crespy V,Pais de Barros JP,Steinmetz E,Venteclef N,Saas P,Lagrost L,Masson D

14-0688-82 was used in Immunohistochemistry (Paraffin) to investigate the role of the LXR cholesterol sensors in inflammatory process.

Tue May 19 00:00:00 EDT 2020

Stearoyl-CoA desaturase-1 impairs the reparative properties of macrophages and microglia in the brain.

The Journal of experimental medicine

Bogie JFJ,Grajchen E,Wouters E,Corrales AG,Dierckx T,Vanherle S,Mailleux J,Gervois P,Wolfs E,Dehairs J,Van Broeckhoven J,Bowman AP,Lambrichts I,Gustafsson JÅ,Remaley AT,Mulder M,Swinnen JV,Haidar M,Ellis SR,Ntambi JM,Zelcer N,Hendriks JJA

Published figure using CD68 monoclonal antibody (Product # 14-0688-82) in Immunohistochemistry

Mon May 04 00:00:00 EDT 2020

Stearoyl-CoA desaturase-1 impairs the reparative properties of macrophages and microglia in the brain.

The Journal of experimental medicine

Published figure using CD68 monoclonal antibody (Product # 14-0688-82) in Immunohistochemistry

Mon May 04 00:00:00 EDT 2020

Stearoyl-CoA desaturase-1 impairs the reparative properties of macrophages and microglia in the brain.

The Journal of experimental medicine

Published figure using CD68 monoclonal antibody (Product # 14-0688-82) in Immunohistochemistry

Mon May 04 00:00:00 EDT 2020

Evaluation of lipid coverage and high spatial resolution MALDI-imaging capabilities of oversampling combined with laser post-ionisation.

Analytical and bioanalytical chemistry

Bowman AP,Bogie JFJ,Hendriks JJA,Haidar M,Belov M,Heeren RMA,Ellis SR

Published figure using CD68 monoclonal antibody (Product # 14-0688-82) in Immunohistochemistry

Wed Apr 01 00:00:00 EDT 2020

Tumor-Derived Retinoic Acid Regulates Intratumoral Monocyte Differentiation to Promote Immune Suppression.

Cell

Devalaraja S,To TKJ,Folkert IW,Natesan R,Alam MZ,Li M,Tada Y,Budagyan K,Dang MT,Zhai L,Lobel GP,Ciotti GE,Eisinger-Mathason TSK,Asangani IA,Weber K,Simon MC,Haldar M

14-0688 was used in Flow cytometry/Cell sorting to find that the TME induces tumor cells to produce retinoic acid (RA), which polarizes intratumoral monocyte differentiation toward TAMs and away from DCs via suppression of DC-promoting transcription factor Irf4.